LOCUS BC003068 1878 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens solute carrier family 19 (folate transporter), member
1, mRNA (cDNA clone MGC:2165 IMAGE:3509422), complete cds.
ACCESSION BC003068
VERSION BC003068.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1878)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1878)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (12-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC003068.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 7 Row: f Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506992.
FEATURES Location/Qualifiers
source 1..1878
/db_xref="H-InvDB:HIT000031401"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2165 IMAGE:3509422"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1878
/gene="SLC19A1"
/gene_synonym="CHMD"
/gene_synonym="FOLT"
/gene_synonym="IFC1"
/gene_synonym="REFC"
/gene_synonym="RFC1"
/db_xref="GeneID:6573"
/db_xref="MIM:600424"
CDS 99..1568
/gene="SLC19A1"
/gene_synonym="CHMD"
/gene_synonym="FOLT"
/gene_synonym="IFC1"
/gene_synonym="REFC"
/gene_synonym="RFC1"
/codon_start=1
/product="SLC19A1 protein"
/protein_id="AAH03068.1"
/db_xref="GeneID:6573"
/db_xref="MIM:600424"
/translation="MVPSSPAVEKQVPVEPGPDPELRSWRRLVCYLCFYGFMAQIRPG
ESFITPYLLGPDKNFTREQVTNEITPVLSYSYLAVLVPVFLLTDYLRYTPVLLLQGLS
FVSVWLLLLLGHSVAHMQLMELFYSVTMAARIAYSSYIFSLVRPARYQRVAGYSRAAV
LLGVFTSSVLGQLLVTVGRVSFSTLNYISLAFLTFSVVLALFLKRPKRSLFFNRDDRG
RCETSASELERMNPGPGGKLGHALRVACGDSVLARMLRELGDSLRRPQLRLWSLWWVF
NSAGYYLVVYYVHILWNEVDPTTNSARVYNGAADAASTLLGAITSFAAGFVKIRWARW
SKLLIAGVTATQAGLVFLLAHTRHPSSIWLCYAAFVLFRGSYQFLVPIATFQIASSLS
KELCALVFGVNTFFATIVKTIITFIVSDVRGLGLPVRKQNEELHVASLSLWKSHLRLA
ADTLSSEGSSGSGPRSWFLSPTLRAALHGPVCPSEVCPS"
misc_feature 165..1391
/gene="SLC19A1"
/gene_synonym="CHMD"
/gene_synonym="FOLT"
/gene_synonym="IFC1"
/gene_synonym="REFC"
/gene_synonym="RFC1"
/note="Folate_carrier; Region: Reduced folate carrier. The
reduced folate carrier (a transmembrane glycoprotein)
transports reduced folate into mammalian cells via the
carrier mediated mechanism (as opposed to the receptor
mediated mechanism) it also transports cytotoxic folate
analogues used in chemotherapy, such as methotrexate
(MTX). Mammalian cells have an absolute requirement for
exogenous folates which are needed for growth, and
biosynthesis of macromolecules"
/db_xref="CDD:pfam01770"
BASE COUNT 306 a 616 c 571 g 385 t
ORIGIN
1 gcggggccgg gtccgggagc cccagggcag ccgccccgcc gagtcgcagg cacagtgtca
61 ccttcgtccc ctccggagct gcacgtggcc tgagcaggat ggtgccctcc agcccagcgg
121 tggagaagca ggtgcccgtg gaacctgggc ctgaccccga gctccggtcc tggcggcgcc
181 tcgtgtgcta cctttgcttc tacggcttca tggcgcagat acggccaggg gagagcttca
241 tcacccccta cctcctgggg cccgacaaga acttcacgcg ggagcaggtc acgaacgaga
301 tcacgccggt gctgtcgtac tcctacctgg ccgtgctggt gcccgtgttc ctgctcaccg
361 actacctgcg ctacacgccg gtgctgctgc tgcaggggct cagcttcgtg tcggtgtggc
421 tgctgctgct gctgggccac tcggtggcgc acatgcagct catggagctc ttctacagcg
481 tcaccatggc cgcgcgcatc gcctattcct cctacatctt ctctctcgtg cggcccgcgc
541 gctaccagcg tgtggccggc tactcgcgcg ctgcggtgct gctgggcgtg ttcaccagct
601 ccgtgctggg ccagctgctg gtcactgtgg gccgagtctc cttctccacg ctcaactaca
661 tctcgctggc cttcctcacc ttcagcgtgg tcctcgccct cttcctgaag cgccccaagc
721 gcagcctctt cttcaaccgc gacgaccggg ggcggtgcga aacctcggct tcggagctgg
781 agcgcatgaa tcccggccca ggcgggaagc tgggacacgc cctgcgggtg gcctgtgggg
841 actcagtgct ggcgcggatg ctgcgggagc tgggggacag cctgcggcgg ccgcagctgc
901 gcctgtggtc cctctggtgg gtcttcaact cggccggcta ctacctggtg gtctactacg
961 tgcacatcct gtggaacgag gtggacccca ccaccaacag tgcgcgggtc tacaacggcg
1021 cggcagatgc tgcctccacg ctgctgggcg ccatcacgtc cttcgccgcg ggcttcgtga
1081 agatccgctg ggcgcgctgg tccaagctgc tcatcgcggg cgtcacggcc acgcaggcgg
1141 ggctggtctt ccttctggcg cacacgcgcc acccgagcag catctggctg tgctatgcgg
1201 ccttcgtgct gttccgcggc tcctaccagt tcctcgtgcc catcgccacc tttcagattg
1261 catcttctct gtctaaagag ctctgtgccc tggtcttcgg ggtcaacacg ttctttgcca
1321 ccatcgtcaa gaccatcatc actttcattg tctcggacgt gcggggcctg ggcctcccgg
1381 tccgcaagca gaatgaagaa ctgcatgtgg cttctctgtc tctgtggaaa agccatctca
1441 ggttggcggc agacacattg tcatcagagg ggagcagcgg ctctggtcct cggagctggt
1501 tcctctctcc caccctaagg gcagccctcc atggtcctgt ctgtccttct gaagtgtgtc
1561 catcctgacc tgcgggtcct cagctgctcc cacacttgtg ccagcccgga ggggactggt
1621 cccggtcacc gcggacgtgc tggccttggt atgtgccagg cttgcctggg ctgggcagcc
1681 ttgggggggc tgcctttgtg gtgggcgctg gggaagtacg tcccagcggc ctcagggtct
1741 aaggagcgct agtgccttgc ccacaggtgc gggaccatct gatgtgatgt gaatactctt
1801 cccacataca ttaaacacac ttaagtgaga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1861 aaaaaaaaaa aaaaaaaa
//