LOCUS BC002959 1953 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens APEX nuclease (apurinic/apyrimidinic endonuclease) 2,
mRNA (cDNA clone MGC:1419 IMAGE:3537317), complete cds.
ACCESSION BC002959
VERSION BC002959.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1953)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1953)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 8 Row: b Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 18375506.
FEATURES Location/Qualifiers
source 1..1953
/db_xref="H-InvDB:HIT000031317"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1419 IMAGE:3537317"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1953
/gene="APEX2"
/gene_synonym="APE2"
/gene_synonym="APEXL2"
/gene_synonym="XTH2"
/db_xref="GeneID:27301"
/db_xref="HGNC:HGNC:17889"
CDS 39..1595
/gene="APEX2"
/gene_synonym="APE2"
/gene_synonym="APEXL2"
/gene_synonym="XTH2"
/codon_start=1
/product="APEX nuclease (apurinic/apyrimidinic
endonuclease) 2"
/protein_id="AAH02959.1"
/db_xref="GeneID:27301"
/db_xref="HGNC:HGNC:17889"
/translation="MLRVVSWNINGIRRPLQGVANQEPSNCAAVAVGRILDELDADIV
CLQETKVTRDALTEPLAIVEGYNSYFSFSRNRSGYSGVATFCKDNATPVAAEEGLSGL
FATQNGDVGCYGNMDEFTQEELRALDSEGRALLTQHKIRTWEGKEKTLTLINVYCPHA
DPGRPERLVFKMRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEE
DPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRL
DYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAG
TQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKN
LKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKE
LRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSR
CNFFLWSRPS"
BASE COUNT 426 a 574 c 514 g 439 t
ORIGIN
1 aggttggcgc gggctgggag gtgttccagc cctttaagat gttgcgcgtg gtgagctgga
61 acatcaatgg gattcggaga cccctgcaag gggtggcaaa tcaggaaccc agcaactgtg
121 ccgccgtggc cgtggggcgc attttggacg agctggatgc ggatatcgtc tgtctccagg
181 aaaccaaagt gaccagggat gcactgacag agcccctggc tatcgttgag ggttataact
241 cctatttcag cttcagccgc aaccgtagcg gctattctgg tgtagccacc ttctgtaagg
301 acaatgctac cccagtggct gctgaagaag gcctgagtgg cctgtttgcc acccagaatg
361 gggatgttgg ttgctatgga aacatggatg agtttaccca agaggaactc cgggctctgg
421 atagtgaggg cagggccctc ctcacacagc ataagatccg cacatgggaa ggtaaggaga
481 agaccttgac cctaatcaac gtgtactgcc cccatgcgga ccctgggagg cctgagcggc
541 tagtctttaa gatgcgcttc tatcgtttgc tgcaaatccg agcagaagcc ctcctggcgg
601 caggcagcca tgtgatcatt ctgggtgacc tgaatacagc ccaccgcccc attgaccact
661 gggatgcagt caacctggaa tgctttgaag aggacccagg gcgcaagtgg atggacagct
721 tgctcagtaa cttggggtgc cagtctgcct ctcatgtagg gcccttcatc gatagctacc
781 gctgcttcca accaaagcag gagggggcct tcacctgctg gtcagcagtc actggcgccc
841 gccatctcaa ctatggctcc cggcttgact atgtgctggg ggacaggacc ctggtcatag
901 acacctttca ggcctctttc ctgctgcctg aggtgatggg ctctgaccac tgccctgtgg
961 gtgcagtctt gagtgtgtcc tctgtgcctg caaaacagtg cccacctctg tgcacccgct
1021 tcctccctga gtttgcaggc acccagctca agatccttcg cttcctagtt cctctcgaac
1081 aaagtcctgt gttggagcag tcgacgctgc agcacaacaa tcaaacccgg gtacagacat
1141 gccaaaacaa agcccaagtg cgctcaacca ggcctcagcc cagtcaggtt ggctctagca
1201 gaggccagaa aaacctgaag agctactttc agccctcccc tagctgtccc caagcctctc
1261 ctgacataga gctgcctagc ctaccactga tgagcgccct catgaccccg aagactccag
1321 aagagaaggc agtggccaaa gtggtgaagg ggcaggccaa gacttcagaa gccaaagatg
1381 agaaggagtt acggacctca ttctggaagt ctgtgctggc ggggcccttg cgcacacccc
1441 tctgtggggg ccacagggag ccatgtgtga tgcgtactgt gaagaagcca ggacccaact
1501 tgggccgccg cttctacatg tgtgccaggc cccggggtcc tcccactgac ccctcctccc
1561 ggtgcaactt cttcctctgg agcaggccca gctgaaccaa tggaggcctg gggacatctg
1621 gcatggtcac ccctgcacat gatctgaggc cagctcccct tccctgagct gcctcctgct
1681 tctccctcaa agtctcctac ccttctcttc ctcttttaag ccctctcttc ctcgctttcc
1741 ttcctaccta gctccttgtt ggtgagcttc ttgtgcctta atcctgtgac ccagcccctt
1801 acaccacttt ccaccttcct gtccgaagta cacggacact agctgcccca ggaagttgtg
1861 tgattttaaa tcacttctgt ctttgctgga aagtgtattt gtgcataaat aaagtctgtg
1921 tatttgtttc aaaaaaaaaa aaaaaaaaaa aaa
//