LOCUS BC002880 2524 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens cysteinyl-tRNA synthetase, mRNA (cDNA clone MGC:11246
IMAGE:3940167), complete cds.
ACCESSION BC002880
VERSION BC002880.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2524)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2524)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC002880.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 15 Row: g Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62240990.
FEATURES Location/Qualifiers
source 1..2524
/db_xref="H-InvDB:HIT000031261"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:11246 IMAGE:3940167"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2524
/gene="CARS"
/gene_synonym="CYSRS"
/gene_synonym="MGC:11246"
/db_xref="GeneID:833"
/db_xref="HGNC:HGNC:1493"
/db_xref="MIM:123859"
CDS 71..2317
/gene="CARS"
/gene_synonym="CYSRS"
/gene_synonym="MGC:11246"
/codon_start=1
/product="cysteinyl-tRNA synthetase"
/protein_id="AAH02880.1"
/db_xref="GeneID:833"
/db_xref="HGNC:HGNC:1493"
/db_xref="MIM:123859"
/translation="MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIP
QDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKI
IKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQ
LATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKF
WEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFA
SSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWP
CPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVR
YFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDN
VDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSS
LGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPE
LGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAK
MKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQ
MAQNGSFQ"
BASE COUNT 685 a 609 c 694 g 536 t
ORIGIN
1 ccggggcggc ggttgcatca gattctagga agtgtctgta gccgcagctg cgggtccggg
61 attcccagcc atggcagatt cctccgggca gcagggcaaa ggccggcgtg tgcagcccca
121 gtggtcccct cctgctggga cccagccatg cagactccac ctttacaaca gcctcaccag
181 gaacaaggaa gtgttcatac ctcaagatgg gaaaaaggtg acgtggtatt gctgtgggcc
241 aaccgtctat gacgcatctc acatggggca cgccaggtcc tacatctctt ttgatatctt
301 gagaagagtg ttgaaggatt acttcaaatt tgatgtcttt tattgcatga acattacgga
361 tattgatgac aagatcatca agagggcccg gcagaaccac ctgttcgagc agtatcggga
421 gaagaggcct gaagcggcac agctcttgga ggatgttcag gccgccctga agccattttc
481 agtaaaatta aatgagacca cggatcccga taaaaagcag atgctcgaac ggattcagca
541 cgcagtgcag cttgccacag agccacttga gaaagctgtg cagtccagac tcacgggaga
601 ggaagtcaac agctgtgtgg aggtgttgct ggaagaagcc aaggatttgc tctctgactg
661 gctggattct acacttggct gtgatgtcac tgacaattcc atcttctcca agctgcccaa
721 gttctgggag ggggacttcc acagagacat ggaagctctg aatgttctcc ctccagatgt
781 cttaacccgg gttagtgagt atgtgccaga aattgtgaac tttgtccaga agattgtgga
841 caacggttac ggctatgtct ccaatgggtc tgtctacttt gatacagcga agtttgcttc
901 tagcgagaag cactcctatg ggaagctggt gcctgaggcc gttggagatc agaaagccct
961 tcaagaaggg gaaggtgacc tgagcatctc tgcagaccgc ctgagtgaga agcgctctcc
1021 caacgacttt gccttatgga aggcctctaa gcccggagaa ccgtcctggc cgtgcccttg
1081 gggaaagggt cgtccgggct ggcatatcga gtgctcggcc atggcaggca ccctcctagg
1141 ggcttcgatg gacattcacg gaggtgggtt cgacctccgg ttcccccacc atgacaatga
1201 gctggcacag tcggaggcct actttgaaaa cgactgctgg gtcaggtact tcctgcacac
1261 aggccacctg accattgcag gctgcaaaat gtcaaagtca ctaaaaaact tcatcaccat
1321 taaagatgcc ttgaaaaagc actcagcacg gcagttgcgg ctggccttcc tcatgcactc
1381 gtggaaggac accctggact actccagcaa caccatggag tcagcgcttc aatatgagaa
1441 gttcttgaat gagtttttct taaatgtgaa agatatcctt cgcgctcctg ttgacatcac
1501 tggtcagttt gagaagtggg gagaagaaga agcagaactg aataagaact tttatgacaa
1561 gaagacagca attcacaaag ccctctgtga caatgttgac acccgcaccg tcatggaaga
1621 gatgcgggcc ttggtcagtc agtgcaacct ctatatggca gcccggaaag ccgtgaggaa
1681 gaggcccaac caggctctgc tggagaacat cgccctgtac ctcacccata tgctgaagat
1741 ctttggggcc gtagaagagg acagctccct gggattcccg gtcggagggc ctggaaccag
1801 cctcagtctc gaggccacag tcatgcccta ccttcaggtg ttatcagaat tccgagaagg
1861 agtgcggaag attgcccgag agcaaaaagt ccctgagatt ctgcagctca gcgatgccct
1921 gcgggacaac atcctgcccg agcttggggt gcggtttgaa gaccacgaag gactgcccac
1981 agtggtgaaa ctggtagaca gaaacacctt attaaaagag agagaagaaa agagacgggt
2041 tgaagaggag aagaggaaga agaaagagga ggcggcccgg aggaaacagg aacaagaagc
2101 agcaaagctg gccaagatga agattccccc cagtgagatg ttcttgtcag aaaccgacaa
2161 atactccaag tttgatgaaa atggtctgcc cacacatgac atggagggca aagagctcag
2221 caaagggcaa gccaagaagc tgaagaagct cttcgaggct caggagaagc tctacaagga
2281 atatctgcag atggcccaga atggaagctt ccagtgaggg ggcacaggac tgacttttta
2341 aaccattgtg gactagtggc tgctgtctgc ctcagtgaca atgtcccagc gctcctatca
2401 tgtttacagt cacccttggg tcctaaatta agagttgtgt tcatgtaggt tcgtgtcgtc
2461 gttggctctg agacattgat aataaatttt tctcaacagt gaaaaaaaaa aaaaaaaaaa
2521 aaaa
//