LOCUS BC002878 1304 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens mediator complex subunit 27, mRNA (cDNA clone
IMAGE:3944264), complete cds.
ACCESSION BC002878
VERSION BC002878.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1304)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1304)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 15 Row: e Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 28558978
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1304
/db_xref="H-InvDB:HIT000031260"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3944264"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1304
/gene="MED27"
/gene_synonym="CRAP34"
/gene_synonym="CRSP34"
/gene_synonym="TRAP37"
/db_xref="GeneID:9442"
/db_xref="HGNC:HGNC:2377"
/db_xref="MIM:605044"
CDS 145..357
/gene="MED27"
/gene_synonym="CRAP34"
/gene_synonym="CRSP34"
/gene_synonym="TRAP37"
/codon_start=1
/product="MED27 protein"
/protein_id="AAH02878.1"
/db_xref="GeneID:9442"
/db_xref="HGNC:HGNC:2377"
/db_xref="MIM:605044"
/translation="MRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSE
NHPLHNSGLLSLDPVQDKTPLYSQLL"
BASE COUNT 319 a 353 c 328 g 304 t
ORIGIN
1 gcacgaggga gctgctgtgg cggcggcaac atggcggacg tgataaatgt cagtgtgaac
61 ctggaggcct tttcccaggc cattagtgcc atccaggcgc tgcgctccag cgtgagcagg
121 gtgttcgact gcctgaagga tgggatgcgg aacaaggaga cgctggaggg ccgggagaag
181 gcctttattg cgcacttcca ggacaactta cattcggtca accgggacct caatgagctg
241 gaacgtctga gcaatctggt aggcaagcca tctgagaacc atcctcttca taacagtggg
301 ctgttaagcc tggatcctgt gcaggacaaa actcctctct atagtcaact cctttaagca
361 tataagtggt caaacaagtt gcagtaccat gcaggactag catctggcct tttaaatcag
421 cagtcattga agcgttccgc taatcagatg ggagtatctg ccaaacgtag accaaaggct
481 cagcccacaa ctcttgtcct accacctcaa tatgttgatg atgtgatcag ccgcattgac
541 aggatgtttc ctgaaatgtc catccactta tccagaccca atggaacatc agcaatgctt
601 ctgcttgata tatggtccaa atccaactat caagtattcc agaaggtgac agaccatgcc
661 accactgccc tgctccacta tcagctgccc cagatgccgg atgtcgtggt ccgatccttc
721 atgacctggt taagaagtta cataaagctg ttccaggccc cgtgccagcg ctgcgggaag
781 tttctgcagg acggccttcc cccgacatgg agggatttcc gaaccctcga agccttccat
841 gacacctgcc ggcagtagcc cccacgctgg ccccagcctc agaccccacc cagcaccttc
901 ccagacacgc aggaagccca cagaaggctc agctggttcc tcactgccca gatgtgtaca
961 gctgctcctc cctttcataa agcagcgcca tgtgtgcaga ggccactctt gaagagcaga
1021 ctccctctgt ggctgatggg actaattatt cccactagcc agcggactga aggcaaagaa
1081 gacctttcta gaacctggta gaaggaagct gtgcagcatg ctcctcgtcc atgtgtgtcg
1141 gcagtgctgg tgtctgtcgt ctccgcgagc tgttactgga atgagccctt gtgttcatgg
1201 gtatcgtcat gcggggttct tgtgttttgt ggggcttggg ttttggtaac ttatttttat
1261 aagcaataaa ccttttgtat cctgaaaaaa aaaaaaaaaa aaaa
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