LOCUS BC002475 834 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens profilin 1, mRNA (cDNA clone MGC:2264 IMAGE:3349355),
complete cds.
ACCESSION BC002475
VERSION BC002475.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 834)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 834)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC002475.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: h Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 16753213.
FEATURES Location/Qualifiers
source 1..834
/db_xref="H-InvDB:HIT000030922"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2264 IMAGE:3349355"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..834
/gene="PFN1"
/db_xref="GeneID:5216"
/db_xref="HGNC:HGNC:8881"
/db_xref="MIM:176610"
CDS 123..545
/gene="PFN1"
/codon_start=1
/product="profilin 1"
/protein_id="AAH02475.1"
/db_xref="GeneID:5216"
/db_xref="HGNC:HGNC:8881"
/db_xref="MIM:176610"
/translation="MAGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNIT
PAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVT
VTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY"
BASE COUNT 203 a 254 c 206 g 171 t
ORIGIN
1 cgcggtccgg acggcagcgc gtgccccgag ctctccgcct ccccccgccc gccagccgag
61 gcagctcgag cccagtccgc ggccccagca gcagcgccga gagcagcccc agtagcagcg
121 ccatggccgg gtggaacgcc tacatcgaca acctcatggc ggacgggacc tgtcaggacg
181 cggccatcgt gggctacaag gactcgccct ccgtctgggc cgccgtcccc gggaaaacgt
241 tcgtcaacat cacgccagct gaggtgggtg tcctggttgg caaagaccgg tcaagttttt
301 acgtgaatgg gctgacactt gggggccaga aatgttcggt gatccgggac tcactgctgc
361 aggatgggga atttagcatg gatcttcgta ccaagagcac cggtggggcc cccaccttca
421 atgtcactgt caccaagact gacaagacgc tagtcctgct gatgggcaaa gaaggtgtcc
481 acggtggttt gatcaacaag aaatgttatg aaatggcctc ccaccttcgg cgttcccagt
541 actgacctcg tctgtccctt ccccttcacc gctccccaca gctttgcacc cctttcctcc
601 ccatacacac acaaaccatt ttattttttg ggccattacc ccatacccct tattgctgcc
661 aaaaccacat gggctggggg ccagggctgg atggacagac acctccccct acccatatcc
721 ctcccgtgtg tggttggaaa acttttgttt tttggggttt ttttttttct gaataaaaaa
781 gattctacta acaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//