LOCUS BC002414 2682 bp mRNA linear HUM 30-DEC-2006
DEFINITION Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae),
mRNA (cDNA clone MGC:2587 IMAGE:3162255), complete cds.
ACCESSION BC002414
VERSION BC002414.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2682)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2682)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Oct 8, 2003 this sequence version replaced BC002414.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: a Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41352714.
FEATURES Location/Qualifiers
source 1..2682
/db_xref="H-InvDB:HIT000030875"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2587 IMAGE:3162255"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2682
/gene="VPS35"
/gene_synonym="DKFZp434E1211"
/gene_synonym="DKFZp434P1672"
/gene_synonym="MEM3"
/db_xref="GeneID:55737"
/db_xref="HGNC:HGNC:13487"
/db_xref="MIM:606931"
CDS 7..2397
/gene="VPS35"
/gene_synonym="DKFZp434E1211"
/gene_synonym="DKFZp434P1672"
/gene_synonym="MEM3"
/codon_start=1
/product="vacuolar protein sorting 35 homolog (S.
cerevisiae)"
/protein_id="AAH02414.1"
/db_xref="GeneID:55737"
/db_xref="HGNC:HGNC:13487"
/db_xref="MIM:606931"
/translation="MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALK
HASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAG
NIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNI
LPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRI
LVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHL
QTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVAT
VIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSS
AVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE
IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYL
ILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQ
TISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQ
LAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRN
TDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTI
QVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL"
BASE COUNT 824 a 533 c 575 g 750 t
ORIGIN
1 gtcgccatgc ctacaacaca gcagtcccct caggatgagc aggaaaagct cttggatgaa
61 gccatacagg ctgtgaaggt ccagtcattc caaatgaaga gatgcctgga caaaaacaag
121 cttatggatg ctctaaaaca tgcttctaat atgcttggtg aactccggac ttctatgtta
181 tcaccaaaga gttactatga actttatatg gccatttctg atgaactgca ctacttggag
241 gtctacctga cagatgagtt tgctaaagga aggaaagtgg cagatctcta cgaacttgta
301 cagtatgctg gaaacattat cccaaggctt taccttttga tcacagttgg agttgtatat
361 gtcaagtcat ttcctcagtc caggaaggat attttgaaag atttggtaga aatgtgccgt
421 ggtgtgcaac atcccttgag gggtctgttt cttcgaaatt accttcttca gtgtaccaga
481 aatatcttac ctgatgaagg agagccaaca gatgaagaaa caactggtga catcagtgat
541 tccatggatt ttgtactgct caactttgca gaaatgaaca agctctgggt gcgaatgcag
601 catcagggac atagccgaga tagagaaaaa agagaacgag aaagacaaga actgagaatt
661 ttagtgggaa caaatttggt gcgcctcagt cagttggaag gtgtaaatgt ggaacgttac
721 aaacagattg ttttgactgg catattggag caagttgtaa actgtaggga tgctttggct
781 caagaatatc tcatggagtg tattattcag gttttccctg atgaatttca cctccagact
841 ttgaatcctt ttcttcgggc ctgtgctgag ttacaccaga atgtaaatgt gaagaacata
901 atcattgctt taattgatag attagcttta tttgctcacc gtgaagatgg acctggaatc
961 ccagcggata ttaaactttt tgatatattt tcacagcagg tggctacagt gatacagtct
1021 agacaagaca tgccttcaga ggatgttgta tctttacaag tctctctgat taatcttgcc
1081 atgaaatgtt accctgatcg tgtggactat gttgataaag ttctagaaac aacagtggag
1141 atattcaata agctcaacct tgaacatatt gctaccagta gtgcagtttc aaaggaactc
1201 accagacttt tgaaaatacc agttgacact tacaacaata ttttaacagt cttgaaatta
1261 aaacattttc acccactctt tgagtacttt gactacgagt ccagaaagag catgagttgt
1321 tatgtgctta gtaatgttct ggattataac acagaaattg tctctcaaga ccaggtggat
1381 tccataatga atttggtatc cacgttgatt caagatcagc cagatcaacc tgtagaagac
1441 cctgatccag aagattttgc tgatgagcag agccttgtgg gccgcttcat tcatctgctg
1501 cgctctgagg accctgacca gcagtacttg attttgaaca cagcacgaaa acattttgga
1561 gctggtggaa atcagcggat tcgcttcaca ctgccacctt tggtatttgc agcttaccag
1621 ctggcttttc gatataaaga gaattctaaa gtggatgaca aatgggaaaa gaaatgccag
1681 aagatttttt catttgccca ccagactatc agtgctttga tcaaagcaga gctggcagaa
1741 ttgcccttaa gactttttct tcaaggagca ctagctgctg gggaaattgg ttttgaaaat
1801 catgagacag tcgcatatga attcatgtcc caggcatttt ctctgtatga agatgaaatc
1861 agcgattcca aagcacagct agctgccatc accttgatca ttggcacttt tgaaaggatg
1921 aagtgcttca gtgaagagaa tcatgaacct ctgaggactc agtgtgccct tgctgcatcc
1981 aaacttctaa agaaacctga tcagggccga gctgtgagca cctgtgcaca tctcttctgg
2041 tctggcagaa acacggacaa aaatggggag gagcttcacg gaggcaagag ggtaatggag
2101 tgcctaaaaa aagctctaaa aatagcaaat cagtgcatgg acccctctct acaagtgcag
2161 ctttttatag aaattctgaa cagatatatc tatttttatg aaaaggaaaa tgatgcggta
2221 acaattcagg ttttaaacca gcttatccaa aagattcgag aagacctccc gaatcttgaa
2281 tccagtgaag aaacagagca gattaacaaa cattttcata acacactgga gcatttgcgc
2341 ttgcggcggg aatcaccaga atccgagggg ccaatttatg aaggtctcat cctttaaaaa
2401 ggaaatagct caccatactc ctttccatgt acatccagtg agggttttat tacgctaggt
2461 ttcccttcca tagattgtgc ctttcagaaa tgctgaggta ggtttcccat ttcttacctg
2521 tgatgtgttt tacccagcac ctccggacac tcaccttcag gaccttaata aaattattca
2581 cttggtaagt gttcaagtct ttctgatcac cccaagtagc atgactgatc tgcaatttaa
2641 aattcctgtg atctgtaaaa aaaaaaaaaa aaaaaaaaaa aa
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