LOCUS BC002409 1849 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens actin, beta, mRNA (cDNA clone MGC:8647 IMAGE:2961617),
complete cds.
ACCESSION BC002409
VERSION BC002409.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1849)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1849)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC002409.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: n Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5016088.
FEATURES Location/Qualifiers
source 1..1849
/db_xref="H-InvDB:HIT000030870"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8647 IMAGE:2961617"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1849
/gene="ACTB"
/gene_synonym="PS1TP5BP1"
/db_xref="GeneID:60"
/db_xref="HGNC:HGNC:132"
/db_xref="MIM:102630"
CDS 72..1199
/gene="ACTB"
/gene_synonym="PS1TP5BP1"
/codon_start=1
/product="actin, beta"
/protein_id="AAH02409.1"
/db_xref="GeneID:60"
/db_xref="HGNC:HGNC:132"
/db_xref="MIM:102630"
/translation="MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM
VGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP
VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT
HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCY
VALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE
TTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
BASE COUNT 439 a 536 c 452 g 422 t
ORIGIN
1 cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc gccgcccgtc cacacccgcc
61 gccagctcac catggatgat gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt
121 gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt cttcccctcc atcgtggggc
181 gccccaggca ccagggcgtg atggtgggca tgggtcagaa ggattcctat gtgggcgacg
241 aggcccagag caagagaggc atcctcaccc tgaagtaccc catcgagcac ggcatcgtca
301 ccaactggga cgacatggag aaaatctggc accacacctt ctacaatgag ctgcgtgtgg
361 ctcccgagga gcaccccgtg ctgctgaccg aggcccccct gaaccccaag gccaaccgcg
421 agaagatgac ccagatcatg tttgagacct tcaacacccc agccatgtac gttgctatcc
481 aggctgtgct atccctgtac gcctctggcc gtaccactgg catcgtgatg gactccggtg
541 acggggtcac ccacactgtg cccatctacg aggggtatgc cctcccccat gccatcctgc
601 gtctggacct ggctggccgg gacctgactg actacctcat gaagatcctc accgagcgcg
661 gctacagctt caccaccacg gccgagcggg aaatcgtgcg tgacattaag gagaagctgt
721 gctacgtcgc cctggacttc gagcaagaga tggccacggc tgcttccagc tcctccctgg
781 agaagagcta cgagctgcct gacggccagg tcatcaccat tggcaatgag cggttccgct
841 gccctgaggc actcttccag ccttccttcc tgggcatgga gtcctgtggc atccacgaaa
901 ctaccttcaa ctccatcatg aagtgtgacg tggacatccg caaagacctg tacgccaaca
961 cagtgctgtc tggcggcacc accatgtacc ctggcattgc cgacaggatg cagaaggaga
1021 tcactgccct ggcacccagc acaatgaaga tcaagatcat tgctcctcct gagcgcaagt
1081 actccgtgtg gatcggcggc tccatcctgg cctcgctgtc caccttccag cagatgtgga
1141 tcagcaagca ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa tgcttctagg
1201 cggactatga cttagttgcg ttacaccctt tcttgacaaa acctaacttg cgcagaaaac
1261 aagatgagat tggcatggct ttatttgttt tttttgtttt gttttggttt tttttttttt
1321 ttttggcttg actcaggatt taaaaactgg aacggtgaag gtgacagcag tcggttggag
1381 cgagcatccc ccaaagttca caatgtggcc gaggactttg attgcacatt gttgtttttt
1441 taatagtcat tccaaatatg agatgcattg ttacaggaag tcccttgcca tcctaaaagc
1501 caccccactt ctctctaagg agaatggccc agtcctctcc caagtccaca caggggaggt
1561 gatagcattg ctttcgtgta aattatgtaa tgcaaaattt ttttaatctt cgccttaata
1621 cttttttatt ttgttttatt ttgaatgatg agccttcgtg cccccccttc cccctttttt
1681 gtcccccaac ttgagatgta tgaaggcttt tggtctccct gggagtgggt ggaggcagcc
1741 agggcttacc tgtacactga cttgagacca gttgaataaa agtgcacacc ttaaaaaaaa
1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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