LOCUS BC001890 2748 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens glucokinase (hexokinase 4), mRNA (cDNA clone MGC:1742
IMAGE:3536582), complete cds.
ACCESSION BC001890
VERSION BC001890.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2748)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2748)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-JAN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 8 Row: l Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 15967157.
FEATURES Location/Qualifiers
source 1..2748
/db_xref="H-InvDB:HIT000030725"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1742 IMAGE:3536582"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2748
/gene="GCK"
/gene_synonym="GK"
/gene_synonym="GLK"
/gene_synonym="HHF3"
/gene_synonym="HK4"
/gene_synonym="HKIV"
/gene_synonym="HXKP"
/db_xref="GeneID:2645"
/db_xref="HGNC:HGNC:4195"
/db_xref="MIM:138079"
CDS 471..1868
/gene="GCK"
/gene_synonym="GK"
/gene_synonym="GLK"
/gene_synonym="HHF3"
/gene_synonym="HK4"
/gene_synonym="HKIV"
/gene_synonym="HXKP"
/codon_start=1
/product="glucokinase (hexokinase 4)"
/protein_id="AAH01890.1"
/db_xref="GeneID:2645"
/db_xref="HGNC:HGNC:4195"
/db_xref="MIM:138079"
/translation="MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRG
LRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVK
TKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDID
KGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDH
QCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRL
VDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRF
VSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINR
MRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAA
LVSAVACKKACMLGQ"
BASE COUNT 641 a 777 c 867 g 463 t
ORIGIN
1 gagcaggaaa tgccgagcgg cgcctgagcc ccagggaagc aggctaggat gtgagagaca
61 cagtcacctg cagcctaatt actcaaaagc tgtccccagg tcacagaagg gagaggacat
121 ttcccactga atctgtctga aggacactaa gccccacagc tcaacacaac caggagagaa
181 agcgctgagg acgccaccca agcgcccagc aatggccctg cctggagaac atccaggctc
241 agtgaggaag ggtccagaag ggaatgcttg ccgactcgtt ggagaacaat gaaaaggagg
301 aaactgtgac tgaacctcaa accccaaacc agcccgagga gaaccacatt ctcccaggga
361 cccagggcgg gccgtgaccc ctgcggcgga gaagccttgg atatttccac ttcagaagcc
421 tactggggaa ggctgagggg tcccagctcc ccacgctggc tgctgtgcag atgctggacg
481 acagagccag gatggaggcc gccaagaagg agaaggtaga gcagatcctg gcagagttcc
541 agctgcagga ggaggacctg aagaaggtga tgagacggat gcagaaggag atggaccgcg
601 gcctgaggct ggagacccat gaagaggcca gtgtgaagat gctgcccacc tacgtgcgct
661 ccaccccaga aggctcagaa gtcggggact tcctctccct ggacctgggt ggcactaact
721 tcagggtgat gctggtgaag gtgggagaag gtgaggaggg gcagtggagc gtgaagacca
781 aacaccagat gtactccatc cccgaggacg ccatgaccgg cactgctgag atgctcttcg
841 actacatctc tgagtgcatc tccgacttcc tggacaagca tcagatgaaa cacaagaagc
901 tgcccctggg cttcaccttc tcctttcctg tgaggcacga agacatcgat aagggcatcc
961 ttctcaactg gaccaagggc ttcaaggcct caggagcaga agggaacaat gtcgtggggc
1021 ttctgcgaga cgctatcaaa cggagagggg actttgaaat ggatgtggtg gcaatggtga
1081 atgacacggt ggccacgatg atctcctgct actacgaaga ccatcagtgc gaggtcggca
1141 tgatcgtggg cacgggctgc aatgcctgct acatggagga gatgcagaat gtggagctgg
1201 tggaggggga cgagggccgc atgtgcgtca ataccgagtg gggcgccttc ggggactccg
1261 gcgagctgga cgagttcctg ctggagtatg accgcctggt ggacgagagc tctgcaaacc
1321 ccggtcagca gctgtatgag aagctcatag gtggcaagta catgggcgag ctggtgcggc
1381 ttgtgctgct caggctcgtg gacgaaaacc tgctcttcca cggggaggcc tccgagcagc
1441 tgcgcacacg cggagccttc gagacgcgct tcgtgtcgca ggtggagagc gacacgggcg
1501 accgcaagca gatctacaac atcctgagca cgctggggct gcgaccctcg accaccgact
1561 gcgacatcgt gcgccgcgcc tgcgagagcg tgtctacgcg cgctgcgcac atgtgctcgg
1621 cggggctggc gggcgtcatc aaccgcatgc gcgagagccg cagcgaggac gtaatgcgca
1681 tcactgtggg cgtggatggc tccgtgtaca agctgcaccc cagcttcaag gagcggttcc
1741 atgccagcgt gcgcaggctg acgcccagct gcgagatcac cttcatcgag tcggaggagg
1801 gcagtggccg gggcgcggcc ctggtctcgg cggtggcctg taagaaggcc tgtatgctgg
1861 gccagtgaga gcagtggccg caagcgcagg gaggatgcca cagccccaca gcacccaggc
1921 tccatgggga agtgctcccc acacgtgctc gcagcctggc ggggcaggag gcctggcctt
1981 gtcaggaccc aggccgcctg ccataccgct ggggaacaga gcgggcctct tccctcagtt
2041 tttcggtggg acagccccag ggccctaacg ggggtgcggc aggagcagga acagagactc
2101 tggaagcccc ccacctttct cgctggaatc aatttcccag aagggagttg ctcactcagg
2161 actttgatgc atttccacac tgtcagagct gttggcctcg cctgggccca ggctctggga
2221 aggggtgccc tctggatcct gctgtggcct cacttccctg ggaactcatc ctgtgtgggg
2281 aggcagctcc aacagcttga ccagacctag acctgggcca aaagggcagc caggggctgc
2341 tcatcaccca gtcctggcca ttttcttgcc tgaggctcaa gaggcccagg gagcaatggg
2401 agggggctcc atggaggagg tgtcccaagc tttgaatacc cccagagacc ttttctctcc
2461 cataccatca ctgagtggct tgtgattctg ggatggaccc tcgcagcagg tgcaagagac
2521 agagccccca agcctctgcc ccaaggggcc cacaaagggg agaagggcca gccctacatc
2581 ttcagctccc atagcgctgg ctcaggaaga aaccccaagc agcattcagc acaccccaag
2641 ggacaacccc atcatatgac atgccaccct ctccatgccc aacctaagat tgtgtgggtt
2701 ttttaattaa aaatgttaaa agttttaaaa aaaaaaaaaa aaaaaaaa
//