LOCUS BC001745 1503 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens DNA segment on chromosome 4 (unique) 234 expressed
sequence, mRNA (cDNA clone MGC:3328 IMAGE:3510777), complete cds.
ACCESSION BC001745
VERSION BC001745.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1503)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1503)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 8 Row: g Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 36951161
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1503
/db_xref="H-InvDB:HIT000030619"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3328 IMAGE:3510777"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1503
/gene="D4S234E"
/gene_synonym="D4S234"
/gene_synonym="NEEP21"
/gene_synonym="NSG1"
/gene_synonym="P21"
/db_xref="GeneID:27065"
/db_xref="MIM:607645"
CDS 162..719
/gene="D4S234E"
/gene_synonym="D4S234"
/gene_synonym="NEEP21"
/gene_synonym="NSG1"
/gene_synonym="P21"
/codon_start=1
/product="D4S234E protein"
/protein_id="AAH01745.1"
/db_xref="GeneID:27065"
/db_xref="MIM:607645"
/translation="MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKV
VVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVV
YKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRS
VSPWMSVLSEEKLSEQETEAAEKSA"
BASE COUNT 380 a 380 c 392 g 351 t
ORIGIN
1 gggagcggcc gtggagccca atcgttcgct ccccttcccg ggtccgcgcg cggcgccgcc
61 tccgccattg ctgcgagcag gagcaggaga cgcggagctc ggagcgctga gctgacctgc
121 cggagccggg cgtgggctgc agcctcggag ctcccggaac gatggtgaag ttggggaaca
181 atttcgcaga gaagggcacc aagcagccgc tgctggagga tggcttcgac accattcccc
241 tgatgacgcc cctcgatgtc aatcagctgc agttcccgcc cccggataag gtggtcgtga
301 aaactaagac cgagtatgaa cctgaccgca agaaagggaa agcacgtcct ccccaaattg
361 ctgagttcac cgtcagcatc acggagggtg tcaccgagag gtttaaggtc tccgtgttgg
421 tcctcttcgc cctggccttc ctcacctgcg tcgtcttcct ggttgtctac aaggtgtaca
481 agtatgaccg cgcctgcccc gatgggttcg tcctcaagaa cacccagtgc atcccagaag
541 gcttggagag ctactacgcg gagcaagact ccagtgcccg ggagaaattt tacacagtca
601 taaaccacta caacctggcc aagcagagca tcacgcgctc cgtatcgccc tggatgtcag
661 ttctgtcaga agagaagctg tccgagcagg agactgaagc ggctgagaag tcagcttagc
721 gggatgggca agttccttac aatgtgtcac ttgcaaataa caaagggact ttgagggaca
781 tttcattaaa tataattact gatactttag aggttactca tttacggtgc aattgcttct
841 gtttgctaat gctgctttgc aaataaaact tgctgccgac cacccacggg cataaaatca
901 agtgcatttc agcattgcct aaagagctct gacaccactt ttcatgttaa gatcttcatt
961 tagctccttt actgggattt attggatgct gtaaaaaaat aaatttacac tggatatgcg
1021 aagggtttgg atctcagata aatgcatttt gtggaattga ttttctgaac cgaccctgct
1081 gtctgcaaac cttcctccat agccatatct agagtgatct ctcgctgtgc taagagcaag
1141 cctacttcgc atttcttcct cggccatcag cgggtaacag tgctgactgc tgccaaggtg
1201 cactgtagta aggtgtctgg agacacagac cgtgaccttg gcgcagcggt gtgcatcaga
1261 ggcgtgtgct gagaagggtg gtgttaagct gtggaaaatg actcaagagc aataaatcag
1321 tgccaaagct tccctgcgca tctgaataga cacaagtgaa gcccgtgtgc gcacagtgca
1381 gaggccgagc ctgtatttcc aggtagacgt ggactttatt gactgtgaat tcatttacct
1441 gtaacttctg acatttcact ctgtgcaaat aaagaacatg aggataaaaa aaaaaaaaaa
1501 aaa
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