LOCUS BC001670 2959 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens Friend leukemia virus integration 1, mRNA (cDNA clone
MGC:2252 IMAGE:2989507), complete cds.
ACCESSION BC001670
VERSION BC001670.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2959)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2959)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis M. Staudt, M.D., Ph.D.
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 3 Row: i Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7110592.
FEATURES Location/Qualifiers
source 1..2959
/db_xref="H-InvDB:HIT000030564"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2252 IMAGE:2989507"
/tissue_type="Lymph, Burkitt lymphoma"
/clone_lib="NIH_MGC_8"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2959
/gene="FLI1"
/gene_synonym="EWSR2"
/gene_synonym="SIC-1"
/db_xref="GeneID:2313"
/db_xref="MIM:193067"
CDS 124..1482
/gene="FLI1"
/gene_synonym="EWSR2"
/gene_synonym="SIC-1"
/codon_start=1
/product="FLI1 protein"
/protein_id="AAH01670.1"
/db_xref="GeneID:2313"
/db_xref="MIM:193067"
/translation="MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQ
PHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNY
NSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQ
NMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKE
DPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANP
GSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDK
LSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYH
AHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGS
YY"
misc_feature 463..717
/gene="FLI1"
/gene_synonym="EWSR2"
/gene_synonym="SIC-1"
/note="SAM_PNT; Region: SAM / Pointed domain"
/db_xref="CDD:smart00251"
misc_feature 961..1212
/gene="FLI1"
/gene_synonym="EWSR2"
/gene_synonym="SIC-1"
/note="Ets; Region: Ets-domain"
/db_xref="CDD:pfam00178"
BASE COUNT 885 a 689 c 663 g 722 t
ORIGIN
1 cacgcaggga gggcccaggg cgccagggag gccgcgccgg gctaatccga aggggctgcg
61 aggtcaggct gtaaccgggt caatgtgtgg aatattgggg ggctcggctg cagacttggc
121 caaatggacg ggactattaa ggaggctctg tcggtggtga gcgacgacca gtccctcttt
181 gactcagcgt acggagcggc agcccatctc cccaaggccg acatgactgc ctcggggagt
241 cctgactacg ggcagcccca caagatcaac cccctcccac cacagcagga gtggatcaat
301 cagccagtga gggtcaacgt caagcgggag tatgaccaca tgaatggatc cagggagtct
361 ccggtggact gcagcgttag caaatgcagc aagctggtgg gcggaggcga gtccaacccc
421 atgaactaca acagctatat ggacgagaag aatggccccc ctcctcccaa catgaccacc
481 aacgagagga gagtcatcgt ccccgcagac cccacactgt ggacacagga gcatgtgagg
541 caatggctgg agtgggccat aaaggagtac agcttgatgg agatcgacac atcctttttc
601 cagaacatgg atggcaagga actgtgtaaa atgaacaagg aggacttcct ccgcgccacc
661 accctctaca acacggaagt gctgttgtca cacctcagtt acctcaggga aagttcactg
721 ctggcctata atacaacctc ccacaccgac caatcctcac gattgagtgt caaagaagac
781 ccttcttatg actcagtcag aagaggagct tggggcaata acatgaattc tggcctcaac
841 aaaagtcctc cccttggagg ggcacaaacg atcagtaaga atacagagca acggccccag
901 ccagatccgt atcagatcct gggcccgacc agcagtcgcc tagccaaccc tggaagcggg
961 cagatccagc tgtggcaatt cctcctggag ctgctctccg acagcgccaa cgccagctgt
1021 atcacctggg aggggaccaa cggggagttc aaaatgacgg accccgatga ggtggccagg
1081 cgctggggcg agcggaaaag caagcccaac atgaattacg acaagctgag ccgggccctc
1141 cgttattact atgataaaaa cattatgacc aaagtgcacg gcaaaagata tgcttacaaa
1201 tttgacttcc acggcattgc ccaggctctg cagccacatc cgaccgagtc gtccatgtac
1261 aagtaccctt ctgacatctc ctacatgcct tcctaccatg cccaccagca gaaggtgaac
1321 tttgtccctc cccatccatc ctccatgcct gtcacttcct ccagcttctt tggagccgca
1381 tcacaatact ggacctcccc cacgggggga atctacccca accccaacgt cccccgccat
1441 cctaacaccc acgtgccttc acacttaggc agctactact agaagcttac tcatcagtgg
1501 ccttctagct gaagcccatc ctgcacactt actggatgct ttggactcaa caggacatat
1561 gtggccttga agggaagaca aaactggatg ttctttcttg ttggatagaa cctttgtatt
1621 tgttctttaa aaacattttt tttaatgttg gtaacttttg cttcctctac ctgaacaaag
1681 agatgaataa ttccatgggc cagtatgcca gtttgaattc tcagtctcct agcatcttgt
1741 gagttgcata ttaagattac tggaatggtt aagtcatggt tctgagaaag aagctgtacg
1801 ttttctttat gtttttatga ccaaagcagt tccttgtcaa tacacggggt tcagtatgac
1861 acagaatcat ggacttaacc cgtcatgttc tggtttgaga tttagtgaca aatagaggtg
1921 ggaagcttat aatctaattt taggaggacc aaattcagtg gatggcaact ggaacattga
1981 ttgtaaggcc agtgaagttt tcacccaact ggaatttgat ggaaagaagg tttgtgtgtt
2041 taagacgcca agggcattgc agaatccctc tcagtggaca gtatgcactc agctgaccac
2101 tctctctaga aatagtcaag atatgaacta agaaatttta atgcaaatac atacattcct
2161 gaaagacggg gaattaaatt actaattttt ttttttttta aatgatgaca gtggtcccag
2221 aacttggaaa agttgtaggg atttctaaac tcaagcagat tcgcaagtgc tgtgcgcttg
2281 tcagaccatc agaccagggc caaccaatca gaaggcaact tactgtataa attatgcaga
2341 gttattttcc tatatctcac agtattaaaa ataaataatt aaaaattaag aataaataaa
2401 cgagttgacc tcggtcacaa aagcagtttt actatcgaat caatcgctgt tatttttttt
2461 aatgtaattt gtacatcttt tttcaatctg tacatttggg ctgtctgtat gtttttatag
2521 ctggttttta aaaagcataa tatgcctata gctgaaaagg aaacagggct gtttaagtca
2581 ctgacttatg agaaagcaaa gcactggtac agttatttaa caggcataca caagcaggga
2641 aaagataatc catttagatc tttaatgctt tggaaatgcg tgtaacagta ctgcaataat
2701 cacagctctg ggaaaaacaa cgaaactttc ccttgtggag aggagggatt ttcctgctct
2761 atataagcaa catattttta gacattaaaa tatatataat tttgcaggta attgttgact
2821 tttttaacta tattaagtgt taagctgaca actgtcaaag aagaccatgt tgtaaaataa
2881 tttgactaaa taaatggttc cttctctcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2941 aaaaaaaaaa aaaaaaaaa
//