LOCUS BC000780 1474 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,
assembly factor 1, mRNA (cDNA clone MGC:707 IMAGE:3504355),
complete cds.
ACCESSION BC000780
VERSION BC000780.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1474)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1474)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000780.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 7 Row: i Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 49574509.
FEATURES Location/Qualifiers
source 1..1474
/db_xref="H-InvDB:HIT000029914"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:707 IMAGE:3504355"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1474
/gene="NDUFAF1"
/gene_synonym="CGI-65"
/gene_synonym="CGI65"
/gene_synonym="CIA30"
/db_xref="GeneID:51103"
/db_xref="HGNC:HGNC:18828"
/db_xref="MIM:606934"
CDS 383..1366
/gene="NDUFAF1"
/gene_synonym="CGI-65"
/gene_synonym="CGI65"
/gene_synonym="CIA30"
/codon_start=1
/product="NADH dehydrogenase (ubiquinone) 1 alpha
subcomplex, assembly factor 1"
/protein_id="AAH00780.1"
/db_xref="GeneID:51103"
/db_xref="HGNC:HGNC:18828"
/db_xref="MIM:606934"
/translation="MALVHKLLRGTYFLRKFSKPTSALYPFLGIRFAEYSSSLQKPVA
SPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIV
DHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQS
ALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAFERKMSYDWSQFNTLYLRVRGDGRP
WMVNIKEDTDFFQRTNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNRGRIRDVQHELPL
DKISSIGFTLADKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPELNPRLFK"
BASE COUNT 394 a 286 c 382 g 412 t
ORIGIN
1 aagcggcggc aggccggcgg ttggtgtgtc ccgggtgtgg ggaggcgaca gagccctggc
61 acttgagggt tgagggggcc tccccagcgc ggcgaaccgt ctagcctccg gaggccaggc
121 cgtgagtgcg ggaggtatac gccaaggcgg aagaattttg ccactcacta cctgtgtgac
181 ctcgggtaaa ttagccttgg aacgtcagtt tcttcgtctc tataattgaa ataataatag
241 tacctctctc aggattgttg tgagccgtca gtgaaacact tagagcagtt tctggcacat
301 ggtagaattg ggctatttgc tgaagcttct tggtggccct tgctagccca ggaagaaact
361 tacattttga tttttttgta ccatggcttt ggttcacaaa ttgctgcgtg gtacttattt
421 tctcagaaaa ttctctaagc caacttctgc cttgtatcca tttttgggta ttcgctttgc
481 agagtattcc agtagtcttc agaaaccagt ggcttctcct ggcaaagcct cctcacagag
541 gaagactgaa ggggatttgc aaggagatca ccagaaagaa gttgctttgg atataacttc
601 ttctgaggag aagcctgatg ttagtttcga taaagcaatt agagatgaag caatatacca
661 ttttaggctt ttgaaggatg aaattgtgga tcattggaga ggaccggaag gccaccctct
721 gcatgaggtc ttgctggaac aagccaaggt tgtctggcaa ttccggggga aagaagattt
781 ggataagtgg acagtgactt ctgataagac gattggaggc agaagtgaag tgtttttgaa
841 aatgggcaag aataaccaaa gtgcactgct atatggaact ctgagctctg aggcgcctca
901 ggacggggag tctacccgaa gtgggtactg tgcaatgata tccaggattc caaggggtgc
961 ttttgagagg aagatgtctt acgattggtc ccagttcaat actctgtatc tccgtgtacg
1021 tggggatggt cggccttgga tggtgaatat caaggaggac acagatttct tccagaggac
1081 gaatcagatg tatagttact tcatgttcac ccgcggggga ccctactggc aggaggtcaa
1141 gattcctttt tccaaatttt tcttctctaa tcgaggaaga atccgggatg ttcagcatga
1201 gcttccgctt gataagatct cttctatagg attcaccttg gctgataaag tggatggtcc
1261 attcttcctg gagatagatt ttattggcgt gtttactgat ccagctcata cagaagaatt
1321 tgcctatgaa aattctccag agcttaaccc aaggcttttt aaataaagat catatggtag
1381 ttttgtttta ctaatctaag ggtactagca tctacaatga tatagacaaa ataaaatatt
1441 tctttaatgg catccaaaaa aaaaaaaaaa aaaa
//