LOCUS BC000697 2138 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nucleoporin 85kDa, mRNA (cDNA clone MGC:3092
IMAGE:3349383), complete cds.
ACCESSION BC000697
VERSION BC000697.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2138)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2138)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC000697.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: n Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147385.
FEATURES Location/Qualifiers
source 1..2138
/db_xref="H-InvDB:HIT000029844"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3092 IMAGE:3349383"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2138
/gene="NUP85"
/gene_synonym="FLJ12549"
/gene_synonym="Nup75"
/db_xref="GeneID:79902"
/db_xref="HGNC:HGNC:8734"
/db_xref="MIM:170285"
CDS 72..2042
/gene="NUP85"
/gene_synonym="FLJ12549"
/gene_synonym="Nup75"
/codon_start=1
/product="nucleoporin 85kDa"
/protein_id="AAH00697.1"
/db_xref="GeneID:79902"
/db_xref="HGNC:HGNC:8734"
/db_xref="MIM:170285"
/translation="MEELDGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEK
SEMVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFLGLQRIDEELTGKSRKSQLVRVS
KNYRSVIRACMEEMHQVAIAAKDPANGRQFSSQVSILSAMELIWNLCEILFIEVAPAG
PLLLHLLDWVRLHVCEVDSLSADVLGSENPSKHDSFWNLVTILVLQGRLDEARQMLSK
EADASPASAGICRIMGDLMRTMPILSPGNTQTLTELELKWQHWHEECERYLQDSTFAT
SPHLESLLKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHYYAQSSLDL
FLGGESSPEPLDNILLAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCKLLQSHNL
YFGSNMREFLLLEYASGLFAHPSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKA
LKVLRICEQRQMTEQVRSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLR
DYCERGCFSDLDLIDNLGPAMMLSDRLTFLGKYREFHRMYGEKRFADAASLLLSLMTS
RIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLTSRRPVHGESDTEQLQD
DDIETTKVEMLRLSLARNLARAIIREGSLEGS"
BASE COUNT 528 a 526 c 563 g 521 t
ORIGIN
1 gctctgagcg ggaggcctga gcgggaagca ttggcgtccg agcgacttct aggagcctgg
61 ggttcggcgc tatggaggag ctcgatggcg agccaacagt cactttgatt ccaggcgtga
121 attccaagaa gaaccaaatg tattttgact ggggtccagg ggagatgctg gtatgtgaaa
181 cctccttcaa caaaaaagaa aaatcagaga tggtgccaag ttgccccttt atctatatca
241 tccgtaagga tgtagatgtt tactctcaaa tcttgagaaa actcttcaat gaatcccatg
301 gaatctttct gggcctccag agaattgacg aagagttgac tggaaaatcc agaaaatctc
361 aattggttcg agtgagtaaa aactaccgat cagtcatcag agcatgtatg gaggaaatgc
421 accaggttgc aattgctgct aaagatccag ccaatggccg ccagttcagc agccaggtct
481 ccattttgtc agcaatggag ctcatctgga acctgtgtga gattcttttt attgaagtgg
541 ccccagctgg ccctctcctc ctccatctcc ttgactgggt ccggctccat gtgtgcgagg
601 tggacagttt gtcggcagat gttctgggca gtgagaatcc aagcaaacat gacagcttct
661 ggaacttggt gaccatcttg gtgctgcagg gccggctgga tgaggcccga cagatgctct
721 ccaaggaagc cgatgccagc cccgcctctg caggcatatg ccgaatcatg ggggacctga
781 tgaggacaat gcccattctt agtcctggga acacccagac actgacagag ctggagctga
841 agtggcagca ctggcacgag gaatgtgagc ggtacctcca ggacagcaca ttcgccacca
901 gccctcacct ggagtctctc ttgaagatta tgctgggaga cgaagctgcc ttgttagagc
961 agaaggaact tctgagtaat tggtatcatt tcctagtgac tcggctcttg tactccaatc
1021 ccacagtaaa acccattgat ctgcactact atgcccagtc cagcctggac ctgtttctgg
1081 gaggtgagag cagcccagaa cccctggaca acatcttgtt ggcagccttt gagtttgaca
1141 tccatcaagt aatcaaagag tgcagcatcg ccctgagcaa ctggtggttt gtggcccacc
1201 tgacagacct gctggaccac tgcaagctcc tccagtcaca caacctctat ttcggttcca
1261 acatgagaga gttcctcctg ctggagtacg cctcgggact gtttgctcat cccagcctgt
1321 ggcagctggg ggtcgattac tttgattact gccccgagct gggccgagtc tccctggagc
1381 tgcacattga gcggatacct ctgaacaccg agcagaaagc cctgaaggtg ctgcggatct
1441 gtgagcagcg gcagatgact gaacaagttc gcagcatttg taagatctta gccatgaaag
1501 ccgtccgcaa caatcgcctg ggttctgccc tctcttggag catccgtgct aaggatgccg
1561 cctttgccac gctcgtgtca gacaggttcc tcagggatta ctgtgagcga ggctgctttt
1621 ctgatttgga tctcattgac aacctggggc cagccatgat gctcagtgac cgactgacat
1681 tcctgggaaa gtatcgcgag ttccaccgta tgtacgggga gaagcgtttt gccgacgcag
1741 cttctctcct tctgtccttg atgacgtctc ggattgcccc tcggtctttc tggatgactc
1801 tgctgacaga tgccttgccc cttttggaac agaaacaggt gattttctca gcagaacaga
1861 cttatgagtt gatgcggtgt ctggaggact tgacgtcaag aagacctgtg catggagaat
1921 ctgataccga gcagctccag gatgatgaca tagagaccac caaggtggaa atgctgagac
1981 tttctctggc acgaaatctt gctcgggcaa ttataagaga aggctcactg gaaggttcct
2041 gagaactgct tcaatgtggt atctttgtat ggcaatgtat atagattttt taaaagaata
2101 aatgttgttt tgcaaatgta aaaaaaaaaa aaaaaaaa
//