LOCUS BC000665 1919 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens t-complex 1, mRNA (cDNA clone MGC:2234 IMAGE:3349237),
complete cds.
ACCESSION BC000665
VERSION BC000665.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1919)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1919)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: d Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 57863256.
FEATURES Location/Qualifiers
source 1..1919
/db_xref="H-InvDB:HIT000029818"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2234 IMAGE:3349237"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1919
/gene="TCP1"
/gene_synonym="CCT-alpha"
/gene_synonym="CCT1"
/gene_synonym="CCTa"
/gene_synonym="D6S230E"
/gene_synonym="TCP-1-alpha"
/db_xref="GeneID:6950"
/db_xref="HGNC:HGNC:11655"
/db_xref="MIM:186980"
CDS 126..1796
/gene="TCP1"
/gene_synonym="CCT-alpha"
/gene_synonym="CCT1"
/gene_synonym="CCTa"
/gene_synonym="D6S230E"
/gene_synonym="TCP-1-alpha"
/codon_start=1
/product="t-complex 1"
/protein_id="AAH00665.1"
/db_xref="GeneID:6950"
/db_xref="HGNC:HGNC:11655"
/db_xref="MIM:186980"
/translation="MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKM
LVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLK
NADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKI
IGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNC
VVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQ
KILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANL
EGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSL
HDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPN
TLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTI
VKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND"
BASE COUNT 596 a 355 c 458 g 510 t
ORIGIN
1 gcacgaggcg ctcgggtacg ctccacgccg tcgcagccac tgctgtggtc gccggtcggc
61 cgaggggccg cgatactggt tgcccgcggt gtaagcagaa ttcgacgtgt atcgctgccg
121 tcaagatgga ggggcctttg tccgtgttcg gtgaccgcag cactggggaa acgatccgct
181 cccaaaacgt tatggctgca gcttcgattg ccaatattgt aaaaagttct cttggtccag
241 ttggcttgga taaaatgttg gtggatgata ttggtgatgt aaccattact aacgatggtg
301 caaccatcct gaagttactg gaggtagaac atcctgcagc taaagttctt tgtgagctgg
361 ctgatctgca agacaaagaa gttggagatg gaactacttc agtggttatt attgcagcag
421 aactcctaaa aaatgcagat gaattagtca aacagaaaat tcatcccaca tcagttatta
481 gtggctatcg acttgcttgc aaggaagcag tgcgttatat caatgaaaac ctaattgtta
541 acacagatga actgggaaga gattgcctga ttaatgctgc taagacatcc atgtcttcca
601 aaatcattgg aataaatggt gatttctttg ctaacatggt agtagatgct gtacttgcta
661 ttaaatacac agacataaga ggccagccac gctatccagt caactctgtt aatattttga
721 aagcccatgg gagaagtcaa atggagagta tgctcatcag tggctatgca ctcaactgtg
781 tggtgggatc ccagggcatg cccaagagaa tcgtaaatgc aaaaattgct tgccttgact
841 tcagcctgca aaaaacaaaa atgaagcttg gtgtacaggt ggtcattaca gaccctgaaa
901 aactggacca aattagacag agagaatcag atatcaccaa ggagagaatt cagaagatcc
961 tggcaactgg tgccaatgtt attctaacca ctggtggaat tgatgatatg tgtctgaagt
1021 attttgtgga ggctggtgct atggcagtta gaagagtttt aaaaagggac cttaaacgca
1081 ttgccaaagc ttctggagca actattctgt caaccctggc caatttggaa ggtgaagaaa
1141 cttttgaagc tgcaatgttg ggacaggcag aagaagtggt acaggagaga atttgtgatg
1201 atgagctgat cttaatcaaa aatactaagg ctcgtacgtc tgcatcgatt atcttacgtg
1261 gggcaaatga tttcatgtgt gatgagatgg agcgctcttt acatgatgca ctttgtgtag
1321 tgaagagagt tttggagtca aaatctgtgg ttcccggtgg gggtgctgta gaagcagccc
1381 tttccatata ccttgaaaac tatgcaacca gcatggggtc tcgggaacag cttgcgattg
1441 cagagtttgc aagatcactt cttgttattc ccaatacact agcagttaat gctgcccagg
1501 actccacaga tctggttgca aaattaagag cttttcataa tgaggcccag gttaacccag
1561 aacgtaaaaa tctaaaatgg attggtcttg atttgagcaa tggtaaacct cgagacaaca
1621 aacaagcagg ggtgtttgaa ccaaccatag ttaaagttaa gagtttgaaa tttgcaacag
1681 aagctgcaat caccattctt cgaattgatg atcttattaa attacatcca gaaagtaaag
1741 atgataaaca tggaagttat gaagatgctg ttcactctgg agcccttaat gattgatctg
1801 atgttccttt tatttataac aatgttaaat gcaattgtct tgtaccttga gttgagtatt
1861 acacattaaa gtaaagtaca agctgtaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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