LOCUS BC000654 1779 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens keratin 8, mRNA (cDNA clone MGC:1711 IMAGE:3349233),
complete cds.
ACCESSION BC000654
VERSION BC000654.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1779)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1779)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000654.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: b Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 68226427.
FEATURES Location/Qualifiers
source 1..1779
/db_xref="H-InvDB:HIT000029809"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1711 IMAGE:3349233"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1779
/gene="KRT8"
/gene_synonym="CARD2"
/gene_synonym="CK8"
/gene_synonym="CYK8"
/gene_synonym="K2C8"
/gene_synonym="K8"
/gene_synonym="KO"
/db_xref="GeneID:3856"
/db_xref="HGNC:HGNC:6446"
/db_xref="MIM:148060"
CDS 65..1516
/gene="KRT8"
/gene_synonym="CARD2"
/gene_synonym="CK8"
/gene_synonym="CYK8"
/gene_synonym="K2C8"
/gene_synonym="K8"
/gene_synonym="KO"
/codon_start=1
/product="keratin 8"
/protein_id="AAH00654.3"
/db_xref="GeneID:3856"
/db_xref="HGNC:HGNC:6446"
/db_xref="MIM:148060"
/translation="MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSS
NFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKF
ASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLK
LEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLT
DEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRA
EAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAA
IADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYR
KLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS
SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK"
BASE COUNT 429 a 512 c 530 g 308 t
ORIGIN
1 cttctccgct ccttctagga tctccgcctg gttcggcccg cctgcctcca ctccagcctc
61 taccatgtcc atcagggtga cccagaagtc ctacaaggtg tccacctctg gcccccgggc
121 cttcagcagc cgctcctaca cgagtgggcc cggttcccgc atcagctcct cgagcttctc
181 ccgagtgggc agcagcaact ttcgcggtgg cctgggcggc ggctatggtg gggccagcgg
241 catgggaggc atcaccgcag ttacggtcaa ccagagcctg ctgagccccc ttgtcctgga
301 ggtggacccc aacatccagg ccgtgcgcac ccaggagaag gagcagatca agaccctcaa
361 caacaagttt gcctccttca tagacaaggt acggttcctg gagcagcaga acaagatgct
421 ggagaccaag tggagcctcc tgcagcagca gaagacggct cgaagcaaca tggacaacat
481 gttcgagagc tacatcaaca accttaggcg gcagctggag actctgggcc aggagaagct
541 gaagctggag gcggagcttg gcaacatgca ggggctggtg gaggacttca agaacaagta
601 tgaggatgag atcaataagc gtacagagat ggagaacgaa tttgtcctca tcaagaagga
661 tgtggatgaa gcttacatga acaaggtaga gctggagtct cgcctggaag ggctgaccga
721 cgagatcaac ttcctcaggc agctatatga agaggagatc cgggagctgc agtcccagat
781 ctcggacaca tctgtggtgc tgtccatgga caacagccgc tccctggaca tggacagcat
841 cattgctgag gtcaaggcac agtacgagga tattgccaac cgcagccggg ctgaggctga
901 gagcatgtac cagatcaagt atgaggagct gcagagcctg gctgggaagc acggggatga
961 cctgcggcgc acaaagactg agatctctga gatgaaccgg aacatcagcc ggctccaggc
1021 tgagattgag ggcctcaaag gccagagggc ttccctggag gccgccattg cagatgccga
1081 gcagcgtgga gagctggcca ttaaggatgc caacgccaag ttgtccgagc tggaggccgc
1141 cctgcagcgg gccaagcagg acatggcgcg gcagctgcgt gagtaccagg agctgatgaa
1201 cgtcaagctg gccctggaca tcgagatcgc cacctacagg aagctgctgg agggcgagga
1261 gagccggctg gagtctggga tgcagaacat gagtattcat acgaagacca ccagcggcta
1321 tgcaggtggt ctgagctcgg cctatggggg cctcacaagc cccggcctca gctacagcct
1381 gggctccagc tttggctctg gcgcgggctc cagctccttc agccgcacca gctcctccag
1441 ggccgtggtt gtgaagaaga tcgagacacg tgatgggaag ctggtgtctg agtcctctga
1501 cgtcctgccc aagtgaacag ctgcggcagc ccctcccagc ctacccctcc tgcgctgccc
1561 cagagcctgg gaaggaggcc gctatgcagg gtagcactgg caacaggaga cccacctgag
1621 gctcagccct agccctcagc ccacctgggg agtttactac ctggggaccc cccttgccca
1681 tgcctccagc tacaaaacaa ttcaattgct tttttttttt ggtccaaaat aaaacctcag
1741 ctagctctgc caaaaaaaaa aaaaaaaaaa aaaaaaaaa
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