LOCUS BC000545 1423 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens aminoacylase 1, mRNA (cDNA clone MGC:2251
IMAGE:3162222), complete cds.
ACCESSION BC000545
VERSION BC000545.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1423)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1423)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000545.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: c Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4501900.
FEATURES Location/Qualifiers
source 1..1423
/db_xref="H-InvDB:HIT000029724"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2251 IMAGE:3162222"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1423
/gene="ACY1"
/gene_synonym="ACY1D"
/gene_synonym="ACYLASE"
/db_xref="GeneID:95"
/db_xref="HGNC:HGNC:177"
/db_xref="MIM:104620"
CDS 69..1295
/gene="ACY1"
/gene_synonym="ACY1D"
/gene_synonym="ACYLASE"
/codon_start=1
/product="aminoacylase 1"
/protein_id="AAH00545.1"
/db_xref="GeneID:95"
/db_xref="HGNC:HGNC:177"
/db_xref="MIM:104620"
/translation="MTSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQ
LGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDS
EGYIYARGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQ
RPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEK
LHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFR
VAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDM
NLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYT
RLLPALASVPALPSDS"
BASE COUNT 320 a 410 c 413 g 280 t
ORIGIN
1 cagggaaggg cgctgagagg cgagcgtgag cccagcgaca ggagagtgag ctcaccacgc
61 gcagcgccat gaccagcaag ggtcccgagg aggagcaccc atcggtgacg ctcttccgcc
121 agtacctgcg tatccgcact gtccagccca agcctgacta tggagctgct gtggctttct
181 ttgaggagac agcccgccag ctgggcctgg gctgtcagaa agtagaggtg gcacctggct
241 atgtggtgac cgtgttgacc tggccaggca ccaaccctac actctcctcc atcttgctca
301 actcccacac ggatgtggtg cctgtcttca aggaacattg gagtcacgac ccctttgagg
361 ccttcaagga ttctgagggc tacatctatg ccaggggtgc ccaggacatg aagtgcgtca
421 gcatccagta cctggaagct gtgaggaggc tgaaggtgga gggccaccgg ttccccagaa
481 ccatccacat gacctttgtg cctgatgagg aggttggggg tcaccaaggc atggagctgt
541 tcgtgcagcg gcctgagttc cacgccctga gggcaggctt tgccctggat gagggcatag
601 ccaatcccac tgatgccttc actgtctttt atagtgagcg gagtccctgg tgggtgcggg
661 ttaccagcac tgggaggcca ggccatgcct cacgcttcat ggaggacaca gcagcagaga
721 agctgcacaa ggttgtaaac tccatcctgg cattccggga gaaggaatgg cagaggctgc
781 agtcaaaccc ccacctgaaa gaggggtccg tgacctccgt gaacctgact aagctagagg
841 gtggcgtggc ctataacgtg atacctgcca ccatgagcgc cagctttgac ttccgtgtgg
901 caccggatgt ggacttcaag gcttttgagg agcagctgca gagctggtgc caggcagctg
961 gcgagggggt caccctagag tttgctcaga agtggatgca cccccaagtg acacctactg
1021 atgactcaaa cccttggtgg gcagctttta gccgggtctg caaggatatg aacctcactc
1081 tggagcctga gatcatgcct gctgccactg acaaccgcta tatccgcgcg gtgggggtcc
1141 cagctctagg cttctcaccc atgaaccgca cacctgtgct gctgcacgac cacgatgaac
1201 ggctgcatga ggctgtgttc ctccgtgggg tggacatata tacacgcctg ctgcctgccc
1261 ttgccagtgt gcctgccctg cccagtgaca gctgagccct ggaactccta aacctttgcc
1321 cctggggctt ccatcccaac cagtgccaag gacctcctct tcccccttcc aaataataaa
1381 gtctatggac aggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//