LOCUS BC000517 2657 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens threonyl-tRNA synthetase, mRNA (cDNA clone MGC:8553
IMAGE:2822851), complete cds.
ACCESSION BC000517
VERSION BC000517.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2657)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2657)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000517.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: j Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38202254.
FEATURES Location/Qualifiers
source 1..2657
/db_xref="H-InvDB:HIT000029699"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8553 IMAGE:2822851"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2657
/gene="TARS"
/gene_synonym="MGC9344"
/gene_synonym="ThrRS"
/db_xref="GeneID:6897"
/db_xref="HGNC:HGNC:11572"
/db_xref="MIM:187790"
CDS 81..2252
/gene="TARS"
/gene_synonym="MGC9344"
/gene_synonym="ThrRS"
/codon_start=1
/product="threonyl-tRNA synthetase"
/protein_id="AAH00517.2"
/db_xref="GeneID:6897"
/db_xref="HGNC:HGNC:11572"
/db_xref="MIM:187790"
/translation="MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGR
AELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTP
YQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHI
MGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFER
LEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALK
IHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYF
FHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHY
SENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALT
GLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGD
IEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQ
LPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVV
PVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKI
SGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF"
BASE COUNT 861 a 492 c 624 g 680 t
ORIGIN
1 agcccacctc ccacccgcct cttggctcct ctcctctagg ccgtcgcttt cgggttctct
61 catcgcttcg tcgttcgcca atgtttgagg agaaggccag cagtccttca gggaagatgg
121 gaggcgagga gaagccgatt ggtgctggtg aagagaagca aaaggaagga ggcaaaaaga
181 agaacaaaga aggatctgga gatggaggtc gagctgagtt gaatccttgg cctgaatata
241 tttacacacg tcttgagatg tataatatac taaaagcaga acatgattcc attctggcag
301 aaaaggcaga aaaagatagc aagccaatta aagtcacttt gcctgatggt aaacaggttg
361 atgcggaatc ttggaaaact acaccatatc aaattgcctg tggaattagt caaggcctgg
421 ccgacaacac cgttattgct aaagtaaata atgttgtgtg ggacctggac cgccctctgg
481 aagaagattg taccttggag cttctcaagt ttgaggatga ggaagctcag gcagtgtatt
541 ggcactctag tgctcacata atgggtgaag ccatggaaag agtctatggt ggatgtttat
601 gctacggtcc gccaatagaa aatggattct attatgacat gtacctcgaa gaagggggtg
661 tgtctagcaa tgatttctct tctctggagg ctttgtgtaa gaaaatcatt aaagaaaaac
721 aagcttttga aagactggaa gttaagaaag aaactttact ggcaatgttt aagtacaaca
781 agttcaaatg ccggatattg aatgaaaagg tgaatactcc aactaccaca gtctatagat
841 gtggcccttt gatagatctc tgccggggtc ctcatgttag acacacgggc aaaattaagg
901 ctttaaaaat acacaaaaat tcctccacgt actgggaagg caaagcagat atggagactc
961 tccagagaat ttatggcatt tcattcccag atcctaaaat gttgaaagag tgggagaagt
1021 tccaagagga agctaaaaac cgagatcata ggaaaattgg cagggaccaa gaactatatt
1081 tctttcatga actcagccct ggaagttgct tttttctgcc aaaaggagcc tacatttata
1141 atgcacttat tgaattcatt aggagcgaat ataggaaaag aggattccag gaggtagtca
1201 ccccaaacat cttcaacagc cgactctgga tgacctcggg ccactggcag cactacagcg
1261 agaacatgtt ctcctttgag gtggagaagg agctgtttgc cctgaaaccc atgaactgcc
1321 caggacactg ccttatgttt gatcatcggc caaggtcctg gcgagaactg cctctgcggc
1381 tagctgattt tggggtactt cataggaacg agctgtctgg agcactcaca ggactcaccc
1441 gggtacgaag attccaacag gatgatgctc acatattctg tgccatggag cagattgaag
1501 atgaaataaa aggttgtttg gattttctac gtacggtata tagcgtattt ggattttctt
1561 ttaaactaaa cctttctact cgcccggaaa aattccttgg agatatcgaa gtatgggatc
1621 aagctgagaa acaacttgaa aacagtctga atgaatttgg tgaaaagtgg gagttaaact
1681 ctggagatgg agctttctat ggcccaaaga ttgacataca gattaaagat gcgattgggc
1741 ggtaccacca gtgtgcaacc atccagctgg atttccagtt gcccatcaga tttaatctta
1801 cttatgtaag ccatgatggt gatgataaga aaaggccagt gattgttcat cgagccatct
1861 tgggatcagt ggaaagaatg attgctatcc tcacagaaaa ctatgggggc aaatggccct
1921 tttggctgtc ccctcgccag gtaatggtag ttccagtggg accaacctgt gatgaatatg
1981 cccaaaaggt acgacaacaa ttccacgatg ccaaattcat ggcagacatt gatctggatc
2041 caggctgtac attgaataaa aagattcgaa atgcacagtt agcacagtat aacttcattt
2101 tagttgttgg tgaaaaagag aaaatcagtg gcactgttaa tatccgcaca agagacaata
2161 aggtccacgg ggaacgcacc atttctgaaa ctatcgagcg gctacagcag ctcaaagagt
2221 tccgcagcaa acaggcagaa gaagaatttt aatgaaaaaa ttacccagat tggctccatg
2281 gaaaaggagg aacagcgttt ccgtaaaatt gactttgtac tctgaaaatg tcaatttata
2341 ttgaacttgg aggagtttgg caaagtctga ataggtcaac ctgcaggcgt aactattttt
2401 gacctagtca gtttttaaac aatgtgcatt tgaaggagtt aattaaaaga gagccaataa
2461 aatgatttta ctcattcagt atctgagtac tggaagtgaa acatgaggaa tgctttagtg
2521 taatgtggga gaactttttt gtaaatttaa tgcaattgaa aaagttttca aattcaatta
2581 agataactag aattggatta tggtgtaaaa ataaaaaaaa aatttattca cataaaaaaa
2641 aaaaaaaaaa aaaaaaa
//