LOCUS BC000481 2337 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:8698
IMAGE:2964687), complete cds.
ACCESSION BC000481
VERSION BC000481.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2337)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2337)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000481.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: p Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33286421.
FEATURES Location/Qualifiers
source 1..2337
/db_xref="H-InvDB:HIT000029665"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8698 IMAGE:2964687"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2337
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
CDS 105..1700
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="pyruvate kinase, muscle"
/protein_id="AAH00481.3"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDL
PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDV
VIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 533 a 654 c 659 g 491 t
ORIGIN
1 gaggcagtgg ctccttgcac agcagctgca cgcgccgtgg ctccggatct cttcgtcttt
61 gcagcgtagc ccgagtcggt cagcgccgga ggacctcagc agccatgtcg aagccccata
121 gtgaagccgg gactgccttc attcagaccc agcagctgca cgcagccatg gctgacacat
181 tcctggagca catgtgccgc ctggacattg attcaccacc catcacagcc cggaacactg
241 gcatcatctg taccattggc ccagcttccc gatcagtgga gacgttgaag gagatgatta
301 agtctggaat gaatgtggct cgtctgaact tctctcatgg aactcatgag taccatgcgg
361 agaccatcaa gaatgtgcgc acagccacgg aaagctttgc ttctgacccc atcctctacc
421 ggcccgttgc tgtggctcta gacactaaag gacctgagat ccgaactggg ctcatcaagg
481 gcagcggcac tgcagaggtg gagctgaaga agggagccac tctcaaaatc acgctggata
541 acgcctacat ggaaaagtgt gacgagaaca tcctgtggct ggactacaag aacatctgca
601 aggtggtgga agtgggcagc aagatctacg tggatgatgg gcttatttct ctccaggtga
661 agcagaaagg tgccgacttc ctggtgacgg aggtggaaaa tggtggctcc ttgggcagca
721 agaagggtgt gaaccttcct ggggctgctg tggacttgcc tgctgtgtcg gagaaggaca
781 tccaggatct gaagtttggg gtcgagcagg atgttgatat ggtgtttgcg tcattcatcc
841 gcaaggcatc tgatgtccat gaagttagga aggtcctggg agagaaggga aagaacatca
901 agattatcag caaaatcgag aatcatgagg gggttcggag gtttgatgaa atcctggagg
961 ccagtgatgg gatcatggtg gctcgtggtg atctaggcat tgagattcct gcagagaagg
1021 tcttccttgc tcagaagatg atgattggac ggtgcaaccg agctgggaag cctgtcatct
1081 gtgctactca gatgctggag agcatgatca agaagccccg ccccactcgg gctgaaggca
1141 gtgatgtggc caatgcagtc ctggatggag ccgactgcat catgctgtct ggagaaacag
1201 ccaaagggga ctatcctctg gaggctgtgc gcatgcagca cctgattgcc cgtgaggcag
1261 aggctgccat ctaccacttg caattatttg aggaactccg ccgcctggcg cccattacca
1321 gcgaccccac agaagccacc gccgtgggtg ccgtggaggc ctccttcaag tgctgcagtg
1381 gggccataat cgtcctcacc aagtctggca ggtctgctca ccaggtggcc agataccgcc
1441 cacgtgcccc catcattgct gtgacccgga atccccagac agctcgtcag gcccacctgt
1501 accgtggcat cttccctgtg ctgtgcaagg acccagtcca ggaggcctgg gctgaggacg
1561 tggacctccg ggtgaacttt gccatgaatg ttggcaaggc ccgaggcttc ttcaagaagg
1621 gagatgtggt cattgtgctg accggatggc gccctggctc cggcttcacc aacaccatgc
1681 gtgttgttcc tgtgccgtga tggaccccag agcccctcct ccagcccctg tcccaccccc
1741 ttcccccagc ccatccatta ggccagcaac gcttgtagaa ctcactctgg gctgtaacgt
1801 ggcactggta ggttgggaca ccagggaaga agatcaacgc ctcactgaaa catggctgtg
1861 tttgcagcct gctctagtgg gacagcccag agcctggctg cccatcatgt ggccccaccc
1921 aatcaaggga agaaggagga atgctggact ggaggcccct ggagccagat ggcaagaggg
1981 tgacagcttc ctttcctgtg tgtactctgt ccagttcctt tagaaaaaat ggatgcccag
2041 aggactccca accctggctt ggggtcaaga aacagccagc aagagttagg ggccttaggg
2101 cactgggctg ttgttccatt gaagccgact ctggccctgg cccttacttg cttctctagc
2161 tctctaggcc tctccagttt gcacctgtcc ccaccctcca ctcagctgtc ctgcagcaaa
2221 cactccaccc tccaccttcc attttccccc actactgcag cacctccagg cctgttgcta
2281 tagagcctac ctgtatgtca ataaacaaca gctgaagcaa aaaaaaaaaa aaaaaaa
//