LOCUS BC000480 1760 bp mRNA linear HUM 17-DEC-2007
DEFINITION Homo sapiens NEDD8 activating enzyme E1 subunit 1, mRNA (cDNA clone
MGC:8690 IMAGE:2964638), complete cds.
ACCESSION BC000480
VERSION BC000480.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1760)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1760)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000480.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: p Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 66363682.
FEATURES Location/Qualifiers
source 1..1760
/db_xref="H-InvDB:HIT000029664"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8690 IMAGE:2964638"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1760
/gene="NAE1"
/gene_synonym="A-116A10.1"
/gene_synonym="HPP1"
/gene_synonym="ula-1"
/db_xref="GeneID:8883"
/db_xref="HGNC:HGNC:621"
/db_xref="MIM:603385"
CDS 9..1613
/gene="NAE1"
/gene_synonym="A-116A10.1"
/gene_synonym="HPP1"
/gene_synonym="ula-1"
/codon_start=1
/product="NEDD8 activating enzyme E1 subunit 1"
/protein_id="AAH00480.1"
/db_xref="GeneID:8883"
/db_xref="HGNC:HGNC:621"
/db_xref="MIM:603385"
/translation="MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTE
ILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSG
SFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVG
YMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVII
AKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTAL
NTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIA
DSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRV
VRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQV
EEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKI
ITKQFVIFNNTYIYSGMSQTSATFQL"
BASE COUNT 589 a 300 c 375 g 496 t
ORIGIN
1 gcgcggccat ggcgcagctg ggaaagctgc tcaaggagca gaagtacgac cggcagctga
61 ggttgtgggg tgatcatggg caagaggctt tagaatctgc tcatgtttgc ctaataaatg
121 caacagccac aggaactgaa attcttaaaa acttggtact accaggtatt ggttcgttta
181 caattattga tggaaatcag gtcagcggag aagatgctgg aaacaatttc ttccttcaaa
241 gaagcagtat cggcaagaac cgagctgaag ctgccatgga attcttacaa gaattaaata
301 gcgatgtctc tggaagtttt gtggaagaga gtccagaaaa ccttctagac aatgatccct
361 catttttctg taggtttact gttgtagttg caactcagct tcctgaaagc acttcactac
421 gcttagcaga tgtcctctgg aattcccaga ttcctctttt gatctgtagg acatatggac
481 tagttggtta tatgaggatc attataaaag aacatccagt aatagaatct catccagata
541 atgcattaga ggatctacga ctagataagc catttcctga actgagagaa cattttcagt
601 cctatgattt ggatcatatg gaaaaaaagg accacagtca tactccatgg attgtgatca
661 tagctaaata tttagcacag tggtatagtg aaacaaatgg acgaatacct aaaacgtata
721 aagaaaaaga ggacttcaga gatttgatta gacaaggaat tctaaaaaat gaaaatgggg
781 ctccagaaga tgaagagaat tttgaagaag ctattaaaaa tgtgaacaca gcactaaata
841 caactcagat cccaagcagt attgaagata tatttaatga tgatcgctgc ataaatatca
901 ccaaacagac tccatcattt tggattttag ctcgtgcctt aaaggaattt gtggccaaag
961 agggtcaagg aaatttacct gttcgaggca caattcctga tatgattgca gattcaggca
1021 aatatataaa actgcaaaac gtttaccgtg aaaaagcaaa gaaagatgct gccgctgtgg
1081 gtaatcatgt tgccaaattg ctgcagtcca ttggccaggc accagagtcc atttcagaga
1141 aagaattaaa attactctgc agcaattctg catttcttcg agtggtaaga tgtcgatcct
1201 tagctgaaga atatggtttg gatacaatta acaaggatga aattatttct agcatggaca
1261 atccagataa tgaaatagtg ttgtacttaa tgttacgggc tgttgataga tttcataaac
1321 aacagggtag atatccagga gtatctaact atcaagttga agaagatata ggaaagttga
1381 agtcttgtct cactggcttc cttcaggaat atggtttatc tgtaatggtg aaagatgatt
1441 atgtccacga attttgccga tatggagctg ctgagccaca taccattgct gcattcttgg
1501 ggggagctgc tgctcaagag gtcatcaaaa taatcaccaa acaatttgta atttttaata
1561 atacttacat ttacagtggc atgtcacaaa cttcagcaac tttccagttg tagagtaagc
1621 aagcacctta agtagtgtgt taatgattga aactgtaatt gccttcgggt tgtgctttag
1681 tctgtaaaat tctaaaggag agctgctaaa ttgttttctt aataaacatt tttctcattt
1741 gtaaaaaaaa aaaaaaaaaa
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