LOCUS BC000439 1462 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase (lipoamide) beta, mRNA (cDNA
clone MGC:8400 IMAGE:2820672), complete cds.
ACCESSION BC000439
VERSION BC000439.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1462)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1462)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000439.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: d Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505686.
FEATURES Location/Qualifiers
source 1..1462
/db_xref="H-InvDB:HIT000029625"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8400 IMAGE:2820672"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1462
/gene="PDHB"
/gene_synonym="PHE1B"
/db_xref="GeneID:5162"
/db_xref="HGNC:HGNC:8808"
/db_xref="MIM:179060"
CDS 11..1090
/gene="PDHB"
/gene_synonym="PHE1B"
/codon_start=1
/product="pyruvate dehydrogenase (lipoamide) beta"
/protein_id="AAH00439.1"
/db_xref="GeneID:5162"
/db_xref="HGNC:HGNC:8808"
/db_xref="MIM:179060"
/translation="MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMD
EELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMA
GLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQ
CFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQS
KDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDM
ETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPY
AKILEDNSIPQVKDIIFAIKKTLNI"
BASE COUNT 417 a 287 c 363 g 395 t
ORIGIN
1 cacgaccaag atggcggcgg tgtctggctt ggtgcggaga ccccttcggg aggtctccgg
61 gctgctgaag aggcgctttc actggaccgc gccggctgcg ctgcaggtga cagttcgtga
121 tgctataaat cagggtatgg atgaggagct ggaaagagat gagaaggtat ttctgcttgg
181 agaagaagtt gcccagtatg atggggcata caaggttagt cgagggctgt ggaagaaata
241 tggagacaag aggattattg acactcccat atcagagatg ggctttgctg gaattgctgt
301 aggtgcagct atggctgggt tgcggcccat ttgtgaattt atgaccttca atttctccat
361 gcaagccatt gaccaggtta taaactcagc tgccaagacc tactacatgt ctggtggcct
421 tcagcctgtg cctatagtct tcagggggcc caatggtgcc tcagcaggtg tagctgccca
481 gcactcacag tgctttgctg cctggtatgg gcactgccca ggcttaaagg tggtcagtcc
541 ctggaattca gaggatgcta aaggacttat taaatcagcc attcgggata acaatccagt
601 ggtggtgcta gagaatgaat tgatgtatgg ggttcctttt gaatttcctc cggaagctca
661 gtcaaaagat tttctgattc ctattggaaa agccaaaata gaaaggcaag gaacacatat
721 aactgtggtt tcccattcaa gacctgtggg ccactgctta gaagctgcag cagtgctatc
781 taaagaagga gttgaatgtg aggtgataaa tatgcgtacc attagaccaa tggacatgga
841 aaccatagaa gccagtgtca tgaagacaaa tcatcttgta actgtggaag gaggctggcc
901 acagtttgga gtaggagctg aaatctgtgc caggatcatg gaaggtcctg cgttcaattt
961 cctggatgct cctgctgttc gtgtcactgg tgctgatgtc cctatgcctt atgcaaagat
1021 tctagaggac aactctatac ctcaggtcaa agacatcata tttgcaataa agaaaacatt
1081 aaatatttag tttggacttg aatatcaagt cgttgaaatt tatttgaaat acttgctggc
1141 actgcacctg gatttgtact gcaagacctg actattcata acggaaaacg atttctaaag
1201 caacagcagg tatttttgta cagggaagtt taaatgtgtt tgtgtatgga aaactctcca
1261 ctctcctccc ctagatgcca tgcttccttt tgtctgttac ggttgccatg ttctttgaat
1321 aacaaattat ataacatttt atcctctctc accacaagga caaagtatgg atgtggcaga
1381 gtcctgatga aagatgtatc caaacaagat aacttatatg tataaaatta aagcatataa
1441 tatacaaaaa aaaaaaaaaa aa
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