LOCUS BC000358 1668 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic
subunit, mRNA (cDNA clone MGC:8666 IMAGE:2964434), complete cds.
ACCESSION BC000358
VERSION BC000358.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1668)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1668)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000358.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: o Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47132573.
FEATURES Location/Qualifiers
source 1..1668
/db_xref="H-InvDB:HIT000029550"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8666 IMAGE:2964434"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1668
/gene="PRKAG1"
/gene_synonym="AMPKG"
/gene_synonym="MGC8666"
/db_xref="GeneID:5571"
/db_xref="HGNC:HGNC:9385"
/db_xref="MIM:602742"
CDS 31..1026
/gene="PRKAG1"
/gene_synonym="AMPKG"
/gene_synonym="MGC8666"
/codon_start=1
/product="protein kinase, AMP-activated, gamma 1
non-catalytic subunit"
/protein_id="AAH00358.1"
/db_xref="GeneID:5571"
/db_xref="HGNC:HGNC:9385"
/db_xref="MIM:602742"
/translation="METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPT
SSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSA
LVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPES
GNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALG
IFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGV
LKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP"
BASE COUNT 444 a 379 c 400 g 445 t
ORIGIN
1 gcgaggaaac caagccgggc gcctcttgca atggagacgg tcatttcttc agatagctcc
61 ccagctgtgg aaaatgagca tcctcaagag accccagaat ccaacaatag cgtgtatact
121 tccttcatga agtctcatcg ctgctatgac ctgattccca caagctccaa attggttgta
181 tttgatacgt ccctgcaggt gaagaaagct ttttttgctt tggtgactaa cggtgtacga
241 gctgcccctt tatgggatag taagaagcaa agttttgtgg gcatgctgac catcactgat
301 ttcatcaata tcctgcaccg ctactataaa tcagccttgg tacagatcta tgagctagaa
361 gaacacaaga tagaaacttg gagagaggtg tatctccagg actcctttaa accgcttgtc
421 tgcatttctc ctaatgccag cttgtttgat gctgtctctt cattaattcg gaacaagatc
481 cacaggctgc cagttattga cccagaatca ggcaatactt tgtacatcct cacccacaag
541 cgcattctga agttcctcaa attgtttatc actgagttcc ccaagccaga gttcatgtcc
601 aagtctctgg aagagctaca gattggcacc tatgccaata ttgctatggt tcgcactacc
661 acccccgtct atgtggctct ggggattttt gtacagcatc gagtctcagc cctgccagtg
721 gtggatgaga aggggcgtgt ggtggacatc tactccaagt ttgatgttat caatctggca
781 gcagaaaaga cctacaacaa cctagatgta tctgtgacta aagccttgca acatcgatca
841 cattactttg agggtgttct caagtgctac ctgcatgaga ctctggagac catcatcaac
901 aggctagtgg aagcagaggt tcaccgactt gtagtggtgg atgaaaatga tgtggtcaag
961 ggaattgtat cactgtctga catcctgcag gccctggtgc tcacaggtgg agagaagaag
1021 ccctgagctg ggggaagggg tcatgcagca ccaggggata tgcccaactc actgcctgct
1081 ggaagctctg tgggaatcag atgaaacttg agggaattgt gactctgttc cctgttcagg
1141 gtcccctgcc cttctatctg ggagctaggg aaggtatggg ggaggaaaga gaatggattt
1201 atagctaccc ttaccctcac acatacactt gaaaaaactt tcagcctagc cagttctagc
1261 ccctgtcctc ttagatatat ccccctttct gggtgaacta taggctctgt gcctctcaga
1321 caaattctga tctctaagag atccccagac ctcacttgcc tctgcctcca tcttggccct
1381 gattcaaccc taagataata gcacaacaaa attcttcata aagatatttt tattcacctg
1441 ttccgtgcta tatggaggag gccaagtcca tttagtgaca tttcttccca taatgtgagt
1501 ggggaggatt gtggggagga ggggctttgg gttcctgtgt ttgtgcatat gaagggagat
1561 gggggttagg tggaggagga gagcagcgtg gttagctaag gttattgctt tttgtggcaa
1621 atctaattaa atgacaggaa tctcaaaaaa aaaaaaaaaa aaaaaaaa
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