LOCUS BC000323 2022 bp mRNA linear HUM 24-JUL-2006
DEFINITION Homo sapiens flap structure-specific endonuclease 1, mRNA (cDNA
clone MGC:8478 IMAGE:2821792), complete cds.
ACCESSION BC000323
VERSION BC000323.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2022)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2022)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000323.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: g Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19718776.
FEATURES Location/Qualifiers
source 1..2022
/db_xref="H-InvDB:HIT000029517"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8478 IMAGE:2821792"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2022
/gene="FEN1"
/gene_synonym="FEN-1"
/gene_synonym="MF1"
/db_xref="GeneID:2237"
/db_xref="HGNC:HGNC:3650"
/db_xref="MIM:600393"
CDS 130..1272
/gene="FEN1"
/gene_synonym="FEN-1"
/gene_synonym="MF1"
/codon_start=1
/product="flap structure-specific endonuclease 1"
/protein_id="AAH00323.1"
/db_xref="GeneID:2237"
/db_xref="HGNC:HGNC:3650"
/db_xref="MIM:600393"
/translation="MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLI
AVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSE
RRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAE
ASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGL
NQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLH
KEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQG
STQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK"
BASE COUNT 531 a 463 c 584 g 444 t
ORIGIN
1 caggccaccc gccgctaagc tgagaaggga gagcgagctt aggaccgcct gcccggggca
61 accccgaacc aagctttagc cgccgaggcc gcgtgtccca aaggccagtc atccctcctc
121 tgtgttgcca tgggaattca aggcctggcc aaactaattg ctgatgtggc ccccagtgcc
181 atccgggaga atgacatcaa gagctacttt ggccgtaagg tggccattga tgcctctatg
241 agcatttatc agttcctgat tgctgttcgc cagggtgggg atgtgctgca gaatgaggag
301 ggtgagacca ccagccacct gatgggcatg ttctaccgca ccattcgcat gatggagaac
361 ggcatcaagc ccgtgtatgt ctttgatggc aagccgccac agctcaagtc aggcgagctg
421 gccaaacgca gtgagcggcg ggctgaggca gagaagcagc tgcagcaggc tcaggctgct
481 ggggccgagc aggaggtgga aaaattcact aagcggctgg tgaaggtcac taagcagcac
541 aatgatgagt gcaaacatct gctgagcctc atgggcatcc cttatcttga tgcacccagt
601 gaggcagagg ccagctgtgc tgccctggtg aaggctggca aagtctatgc tgcggctacc
661 gaggacatgg actgcctcac cttcggcagc cctgtgctaa tgcgacacct gactgccagt
721 gaagccaaaa agctgccaat ccaggaattc cacctgagcc ggattctgca ggagctgggc
781 ctgaaccagg aacagtttgt ggatctgtgc atcctgctag gcagtgacta ctgtgagagt
841 atccggggta ttgggcccaa gcgggctgtg gacctcatcc agaagcacaa gagcatcgag
901 gagatcgtgc ggcgacttga ccccaacaag taccctgtgc cagaaaattg gctccacaag
961 gaggctcacc agctcttctt ggaacctgag gtgctggacc cagagtctgt ggagctgaag
1021 tggagcgagc caaatgaaga agagctgatc aagttcatgt gtggtgaaaa gcagttctct
1081 gaggagcgaa tccgcagtgg ggtcaagagg ctgagtaaga gccgccaagg cagcacccag
1141 ggccgcctgg atgatttctt caaggtgacc ggctcactct cttcagctaa gcgcaaggag
1201 ccagaaccca agggatccac taagaagaag gcaaagactg gggcagcagg gaagtttaaa
1261 aggggaaaat aaatgtgttt ccccattata cctccttcac cccagaatat ttgccgtctt
1321 gtacccttaa gagctacagc tagagaaacc ttcacggggt ggagagagga ttctaaggct
1381 tttctagcgt gacccttttc agtagtgcta gtcccttttt tacttgatct taatggcaag
1441 aaggccacag aggtactttt ccttttttag ctcaggaaaa tatgtcaggc tcaaaccact
1501 tctcaggcag tttaatggac actaagtcca ttgttacatg aaagtgatag atagcaacaa
1561 gttttggaga agagagaggg agataaaagg gggagacaaa agatgtacag aaatgatttc
1621 ctggctggcc aactggtggc cagtgggagg tgatggtgga cctagactgt gcttttctgt
1681 cttgttcagc cttgacccac cttgagagag agccaccagg aaggcgcatc ttagcagatg
1741 ggaggaactg ctgagagaag atgggcagaa agctggagcc cctggagttg gctgtgtctg
1801 tgtttgtgac tgattactgg ctgtgtcttg ggtgggcaga aactcgaact tgctatgtaa
1861 tttgtgtcta gttattcaga ggagtaagat ggtgatgttc acctggcaat cagctgagtt
1921 gagactttgg aataagacac tggttttcat gcgctgtttt tgttttaaag ttatgaagaa
1981 aaaagtcaat aaaattctaa aagtaaaaaa aaaaaaaaaa aa
//