LOCUS BC000289 1871 bp mRNA linear HUM 12-NOV-2003
DEFINITION Homo sapiens eyes absent homolog 2 (Drosophila), mRNA (cDNA clone
IMAGE:2819257), partial cds.
ACCESSION BC000289
VERSION BC000289.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1871)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1871)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000289.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: a Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20149562.
FEATURES Location/Qualifiers
source 1..1871
/db_xref="H-InvDB:HIT000085822"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2819257"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1871
/gene="EYA2"
/gene_synonym="EAB1"
/gene_synonym="MGC10614"
/db_xref="GeneID:2139"
/db_xref="MIM:601654"
CDS <1..1143
/gene="EYA2"
/gene_synonym="EAB1"
/gene_synonym="MGC10614"
/codon_start=1
/product="EYA2 protein"
/protein_id="AAH00289.2"
/db_xref="GeneID:2139"
/db_xref="MIM:601654"
/translation="VHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTY
VLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPS
PAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADT
HLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDW
MRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAELEALTDLWLTHSLKALNL
INSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFG
RKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL"
misc_feature 733..1095
/gene="EYA2"
/gene_synonym="EAB1"
/gene_synonym="MGC10614"
/note="Hydrolase; Region: haloacid dehalogenase-like
hydrolase. This family are structurally different from the
alpha/ beta hydrolase family (pfam00561). This family
includes L-2-haloacid dehalogenase, epoxide hydrolases and
phosphatases. The structure of the family consists of two
domains. One is an inserted four helix bundle, which is
the least well conserved region of the alignment. The rest
of the fold is composed of the core alpha/beta domain"
/db_xref="CDD:pfam00702"
BASE COUNT 466 a 482 c 487 g 436 t
ORIGIN
1 gtgcaccagg actatccttc ctaccccggc ttcccccaga gccagtaccc ccagtattac
61 ggctcatcct acaaccctcc ctacgtcccg gccagcagca tctgcccttc gcccctctcc
121 acgtccacct acgtcctcca ggaggcatct cacaacgtcc ccaaccagag ttccgagtca
181 cttgctggtg aatacaacac acacaatgga ccttccacac cagcgaaaga gggagacaca
241 gacaggccgc accgggcctc cgacgggaag ctccgaggcc ggtctaagag gagcagtgac
301 ccgtccccgg caggggacaa tgagattgag cgtgtgttcg tgtgggactt ggatgagaca
361 ataattattt ttcactcctt actcacgggg acatttgcat ccagatacgg gaaggacacc
421 acgacgtccg tgcgcattgg ccttatgatg gaagagatga tcttcaacct tgcagataca
481 catctgttct tcaatgacct ggaggattgt gaccagatcc acgttgatga cgtctcatca
541 gatgacaatg gccaagattt aagcacatac aacttctccg ctgacggctt ccacagttcg
601 gccccaggag ccaacctgtg cctgggctct ggcgtgcacg gcggcgtgga ctggatgagg
661 aagctggcct tccgctaccg gcgggtgaag gagatgtaca atacctacaa gaacaacgtt
721 ggtgggttga taggcactcc caaaagggag acctggctac agctccgagc tgagctggaa
781 gctctcacag acctctggct gacccactcc ctgaaggcac taaacctcat caactcccgg
841 cccaactgtg tcaatgtgct ggtcaccacc actcaactaa ttcctgccct ggccaaagtc
901 ctgctatatg gcctggggtc tgtgtttcct attgagaaca tctacagtgc aaccaagaca
961 gggaaggaga gctgcttcga gaggataatg cagagattcg gcagaaaagc tgtctacgtg
1021 gtgatcggtg atggtgtgga agaggagcaa ggagcgaaaa agcacaacat gcctttctgg
1081 cggatatcct gccacgcaga cctggaggca ctgaggcacg ccctggagct ggagtattta
1141 tagcaggatc agcagcatct ccacctgcca tctcaccctc agaccccctc gccttcccca
1201 cctccccacc gagaactcca gagacccaga tgttggacac caggaagggg ccccacagcc
1261 gagacgacgt gtccagtgac catctcagaa gccgtccatc agtccaaatg ggggttctga
1321 gaaggaaagt acccaacatt ggcttcggag tatttgactt tggggaaaag ggctggctcg
1381 gagtctagac tcttctgtaa gactcacaga acaaaagcaa ggaattgctg atttgggggg
1441 tgcctggtga tgaggagggg atgggtttgt cttgtcttct ttttaattta tggactagtc
1501 tcattactcc ggaattatgc tcttgtacct gtgtggctgg gtttcttagt cgttggtttg
1561 gtttggtttt ttgaactggt atgtggggtg gttcacagtt ctaatgtaag cactctattc
1621 tccaagttgt gctttgtggg gacaatcatt ctttgaacat tagagaggaa ggcagttcaa
1681 gctgttgaaa agactattgc ttatttttgt ttttaaagac ctacttgacg tcatgtggac
1741 agtgcacgtg ccttacgcta catcttgttt tctaggaaga gggggatgct gggaaggaat
1801 gggtgctttg tgatggataa aaggcattaa ataaaaccac gtttacattt tgaaaaaaaa
1861 aaaaaaaaaa a
//