LOCUS BC000147 2505 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens malic enzyme 2, NAD(+)-dependent, mitochondrial, mRNA
(cDNA clone MGC:5187 IMAGE:2900677), complete cds.
ACCESSION BC000147
VERSION BC000147.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2505)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2505)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: m Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13376994.
FEATURES Location/Qualifiers
source 1..2505
/db_xref="H-InvDB:HIT000029383"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5187 IMAGE:2900677"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2505
/gene="ME2"
/db_xref="GeneID:4200"
/db_xref="MIM:154270"
CDS 202..1641
/gene="ME2"
/codon_start=1
/product="ME2 protein"
/protein_id="AAH00147.1"
/db_xref="GeneID:4200"
/db_xref="MIM:154270"
/translation="MLSRLRVVSTTCTLACRHLHIKEKGKPLMLNPRTNKGMAFTLQE
RQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQD
DIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVV
TDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFY
MGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTF
NDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSE
QEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGV
AGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFG
PVKLTDGRVFTPGQGNNVYIFPGYRIPIC"
misc_feature 466..1035
/gene="ME2"
/note="malic; Region: Malic enzyme, N-terminal domain"
/db_xref="CDD:pfam00390"
BASE COUNT 770 a 481 c 548 g 706 t
ORIGIN
1 ctcggccgct cttcgggccg cctctgcgtg tggggccgcc cgcgccagtg tgagcctgag
61 ctgacggcgg ctccgggagg ctcgcagaag gggagggccg ggcggcgcgg gagctgagca
121 tcgccagggc gggcggcagg gcgcggcctc tccgccgggt gtaccacctg tcgcggcgcg
181 agacctctgg tgaaagaaaa gatgttgtcc cggttaagag tagtttccac cacttgtact
241 ttggcatgtc gacatttgca cataaaagaa aaaggcaagc cacttatgct gaacccaaga
301 acaaacaagg gaatggcatt tactttacaa gaacgacaaa tgcttggtct tcaaggactt
361 ctacctccca aaatagagac acaagatatt caagccttac gatttcatag aaacttgaag
421 aaaatgacta gccctttgga aaaatatatc tacataatgg gaatacaaga aagaaatgag
481 aaattgtttt atagaatact gcaagatgac attgagagtt taatgccaat tgtatataca
541 ccgacggttg gtcttgcctg ctcccagtat ggacacatct ttagaagacc taagggatta
601 tttatttcga tctcagacag aggtcatgtt agatcaattg tggataactg gccagaaaat
661 catgttaagg ctgttgtagt gactgatgga gagagaattc tgggtcttgg agatctgggt
721 gtctatggaa tgggaattcc agtaggaaaa ctttgtttgt atacagcttg tgcaggaata
781 cggcctgata gatgcctgcc agtgtgtatt gatgtgggaa ctgataatat cgcactctta
841 aaagacccat tttacatggg cttgtaccag aaacgagatc gcacacaaca gtatgatgac
901 ctgattgatg agtttatgaa agctattact gacagatatg gccggaacac actcattcag
961 ttcgaagact ttggaaatca taatgcattc aggttcttga gaaagtaccg agaaaaatat
1021 tgtactttca atgatgatat tcaagggaca gctgcagtag ctctagcagg tcttcttgca
1081 gcacaaaaag ttattagtaa accaatctcc gaacacaaaa tcttattcct tggagcagga
1141 gaggctgctc ttggaattgc aaatcttata gttatgtcta tggtagaaaa tggcctgtca
1201 gaacaagagg cacaaaagaa aatctggatg tttgacaagt atggtttatt agttaaggga
1261 cggaaagcaa aaatagatag ttatcaggaa ccatttactc actcagcccc agagagcata
1321 cctgatactt ttgaagatgc agtgaatata ctgaagcctt caactataat tggagttgca
1381 ggtgctggcc gtcttttcac tcctgatgta atcagagcca tggcctctat caatgaaagg
1441 cctgtaatat ttgcattaag taatcctaca gcacaggcag agtgcacggc tgaagaagca
1501 tatacactta cagagggcag gtgtttgttt gccagtggca gtccatttgg gccagtgaaa
1561 cttacagatg ggcgagtctt tacaccaggt caaggaaaca atgtttatat ttttccaggt
1621 tacagaatac ctatatgcta ataaaatggc tttccgatac ccagaacctg aagacaaggc
1681 caaatatgtt aaagaaagaa catggcggag tgaatatgat tccctgctgc cagatgtgta
1741 tgaatggcca gaatctgcat caagccctcc tgtgataaca gaatagaagc actcccctga
1801 taaatacttt ctgtgctcca gggaacccct tttttcagac aagaagagat aatgtcttca
1861 gttttatggt gttttctgtg ttttgttctc cctgaccact ttggttgatg tattttttcc
1921 atgcgtctcc acatctgttg gggtagacgt gttgattgat tgcattgccc accagcaccc
1981 tacaatcaga tagttgtgat gctttaattc taacatacag cccgtaccac atccaggaga
2041 tgtaaaaagt gtgtttgtga atgtcttcac ttgtactcta attcagactt gccaaagtat
2101 ttgctattta ctattatggg taatactctt ctctggccta gttcttacag agctactaaa
2161 atagaaattt acttttatgg atagaagtac agaattttga gaagaaacta aattttcacc
2221 aaattttaag gaaaaattgt cattatctaa aaatgttctt atatatctgc ttcatcttac
2281 cttcatactc tgaaattccc tatagcagac agagctaggg aaatattaaa aatttaccct
2341 atttattttc tggaactaaa tcaagcctta actataacat tatgagagta atgggaacta
2401 ctgctggctt taagtaaata aaagtcattg ttttcaacag tgtataaaaa tcatagtgta
2461 acctttttat ttaataaata tcttacattt aaaaaaaaaa aaaaa
//