LOCUS BC000008 1229 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens N-acetylneuraminic acid synthase, mRNA (cDNA clone
MGC:934 IMAGE:3505324), complete cds.
ACCESSION BC000008
VERSION BC000008.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1229)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1229)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000008.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 7 Row: f Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 12056472.
FEATURES Location/Qualifiers
source 1..1229
/db_xref="H-InvDB:HIT000029276"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:934 IMAGE:3505324"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1229
/gene="NANS"
/gene_synonym="SAS"
/db_xref="GeneID:54187"
/db_xref="HGNC:HGNC:19237"
/db_xref="MIM:605202"
CDS 79..1158
/gene="NANS"
/gene_synonym="SAS"
/codon_start=1
/product="N-acetylneuraminic acid synthase"
/protein_id="AAH00008.1"
/db_xref="GeneID:54187"
/db_xref="HGNC:HGNC:19237"
/db_xref="MIM:605202"
/translation="MPLELELCPGRWVGGQHPCFIIAEIGQNHQGDLDVAKRMIRMAK
ECGADCAKFQKSELEFKFNRKALERPYTSKHSWGKTYGEHKRHLEFSHDQYRELQRYA
EEVGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAKKGRPMVISSGMQ
SMDTMKQVYQIVKPLNPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHE
TGIAISVAAVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSP
TKQLLPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKK
VLVTVEEDDTIMEELVDNHGKKIKS"
BASE COUNT 348 a 286 c 350 g 245 t
ORIGIN
1 cacagaacag agtagaggcg gcggcggcgg cggccggacc cagactggta gtgaggcgtt
61 ggaccccgag ccgctgcaat gccgctggag ctggagctgt gtcccgggcg ctgggtgggc
121 gggcaacacc cgtgcttcat cattgccgag atcggccaga accaccaggg cgacctggat
181 gtagccaagc gcatgatccg catggccaag gagtgtgggg ctgattgtgc caagttccag
241 aagagtgagc tagaattcaa gtttaatcgg aaagccttgg agaggccata cacctcgaag
301 cattcctggg ggaagacgta cggggagcac aaacgacatc tggagttcag ccatgaccag
361 tacagggagc tgcagaggta cgccgaggag gttgggatct tcttcactgc ctctggcatg
421 gatgagatgg cagttgaatt cctgcatgaa ctgaatgttc catttttcaa agttggatct
481 ggagacacta ataattttcc ttatctggaa aagacagcca aaaaaggtcg cccaatggtg
541 atctccagtg ggatgcagtc aatggacacc atgaagcaag tttatcagat cgtgaagccc
601 ctcaacccca acttctgctt cttgcagtgt accagcgcat acccgctcca gcctgaggac
661 gtcaacctgc gggtcatctc ggaatatcag aagctctttc ctgacattcc catagggtat
721 tctgggcatg aaacaggcat agcgatatct gtggccgcag tggctctggg ggccaaggtg
781 ttggaacgtc acataacttt ggacaagacc tggaagggga gtgaccactc ggcctcgctg
841 gagcctggag aactggccga gctggtgcgg tcagtgcgtc ttgtggagcg tgccctgggc
901 tccccaacca agcagctgct gccctgtgag atggcctgca atgagaagct gggcaagtct
961 gtggtggcca aagtgaaaat tccggaaggc accattctaa caatggacat gctcaccgtg
1021 aaggtgggtg agcccaaagg ctatcctcct gaagacatct ttaatctagt gggcaagaag
1081 gtcctggtca ctgttgaaga ggatgacacc atcatggaag aattggtaga taatcatggc
1141 aaaaaaatca agtcttaaaa ataaagtgcc attctctgaa aaaaaaaaaa aaaaaaaaaa
1201 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//