LOCUS AY756064 1491 bp mRNA linear HUM 18-OCT-2004
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
splice isoform 5 mRNA, complete cds, alternatively spliced.
ACCESSION AY756064
VERSION AY756064.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1491)
AUTHORS Minchenko,O., Opentanova,I., Minchenko,D., Ogura,T. and Esumi,H.
TITLE Hypoxia induces transcription of
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4 gene via
hypoxia-inducible factor-1alpha activation
JOURNAL FEBS Lett. 576 (1-2), 14-20 (2004)
PUBMED 15474002
REFERENCE 2 (bases 1 to 1491)
AUTHORS Minchenko,D., Opentanova,I. and Minchenko,O.
TITLE Direct Submission
JOURNAL Submitted (22-SEP-2004) Molecular Biology, Palladin Institute of
Biochemistry NAS, Leontovycha 9, Kyiv 01601, Ukraine
FEATURES Location/Qualifiers
source 1..1491
/db_xref="H-InvDB:HIT000257635"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3p22-p21"
/cell_line="DB-1"
/cell_type="melanoma"
CDS 114..1418
/EC_number="3.1.3.46"
/EC_number="2.7.1.105"
/function="catalyzes synthesis and degradation of
fructose-2,6-biphosphate"
/note="alternatively spliced; bifunctional enzyme with
kinase and phosphatase activities; PFK-2;
PFK-2/F-2,7BPase; PKFKB; contains deletion in
biphosphatase domain"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 4 splice isoform 5"
/protein_id="AAV28719.1"
/translation="MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVM
VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKI
RKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICV
DPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKI
MDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSP
RGREFAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDASYEDL
VQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKLTPVA
YGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ"
BASE COUNT 334 a 412 c 447 g 298 t
ORIGIN
1 gggctgtcgc ggcttgagga ggggcggggc ccaaggggcg ggacctcggg tggcccgagg
61 tggcccggct gctgggttgg aagcggcgca gtccgactca tcccggcccc gggatggcgt
121 ccccacggga attgacacag aaccccctga agaagatctg gatgccatac agcaatgggc
181 ggcccgctct gcacgcttgc cagcgcggtg tgtgcatgac caactgccca actctcattg
241 tcatggtggg cctgcccgcc aggggcaaga cctacatctc caagaagctg actcgatacc
301 tgaactggat tggtgtgccc actcgggagt tcaatgttgg ccagtatcgc cgggacgtgg
361 tcaagaccta caaatctttt gaattttttc tccccgacaa tgaagagggc ctgaaaatca
421 ggaagcagtg tgccctggca gccctccgtg acgtccggcg gttccttagt gaggaggggg
481 gacatgtggc ggtttttgat gccacaaaca ccacccgaga acggagagcg accatcttta
541 attttggaga acagaatggc tacaagacct tttttgtcga gtccatctgt gtggatcctg
601 aggtcatagc tgccaacatc gtgcaagtga aactgggcag ccctgactat gtcaaccgcg
661 acagtgatga ggctacggag gacttcatga ggcgcattga gtgctatgag aactcctacg
721 agtcgctaga tgaggacctg gatagggacc tgtcctatat caagatcatg gatgtgggcc
781 agagctacgt ggtgaaccgt gtggctgacc acatccagag ccgcatcgta tattacctca
841 tgaacatcca cgtgaccccc cgctccatct acctctgccg gcacggggag agcgagctca
901 acctcaaggg ccggattggc ggggacccag gactgtcccc tcggggcagg gagtttgcca
961 agagtctagc ccagttcatc agtgaccaaa atatcaagga tctgaaggtc tggacaagcc
1021 agatgaagag gacaatccag acggctgagg cactgggtgt gccctatgaa cagtggaagg
1081 tcctcaacga gatcgatgcg tcctacgagg acctggtcca gagactggag cctgtcatca
1141 tggagctgga gaggcaagag aatgtgctgg tcatctgcca ccaggctgtg atgcgctgcc
1201 tgctggccta cttcctcgac aaggcagcag aacagctgcc ctacctcaag tgtccgctgc
1261 acacagtcct gaagctgact cctgtggcat atggttgtaa agtggagtcc atattcctga
1321 acgtggctgc tgtgaacacg caccgggaca ggcctcagaa cgtggacatc tcaagacctc
1381 cagaggaagc ccttgtcacg gtgcctgctc accagtgacc atgttcatcc actgtgacca
1441 ctaggcaggc actgctctct gcagaggggg tcattccagg ccctccagtg t
//