LOCUS AY756063 1724 bp mRNA linear HUM 18-OCT-2004
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
splice isoform 4 mRNA, complete cds, alternatively spliced.
ACCESSION AY756063
VERSION AY756063.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1724)
AUTHORS Minchenko,O., Opentanova,I., Minchenko,D., Ogura,T. and Esumi,H.
TITLE Hypoxia induces transcription of
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4 gene via
hypoxia-inducible factor-1alpha activation
JOURNAL FEBS Lett. 576 (1-2), 14-20 (2004)
PUBMED 15474002
REFERENCE 2 (bases 1 to 1724)
AUTHORS Minchenko,O., Minchenko,D. and Opentanova,I.
TITLE Direct Submission
JOURNAL Submitted (20-SEP-2004) Molecular Biology, Palladin Institute of
Biochemistry NAS, Leontovycha 9, Kyiv 01601, Ukraine
FEATURES Location/Qualifiers
source 1..1724
/db_xref="H-InvDB:HIT000257634"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3p22-p21"
/cell_line="DB-1"
/cell_type="melanoma"
misc_difference 424..553
/replace=""
CDS 815..1651
/EC_number="3.1.3.46"
/EC_number="2.7.1.105"
/function="catalyzes synthesis and degradation of
fructose-2,6-biphosphate"
/note="alternatively spliced; bifunctional enzyme with
kinase and phosphatase activities; PFK-2;
PFK-2/F-2,7BPase; PKFKB; isoform can not produce
functionally active kinase isozyme"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 4 splice isoform 4"
/protein_id="AAV28718.1"
/translation="MRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQ
SRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQN
IKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALR
DQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQ
LPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPA
HQ"
BASE COUNT 406 a 471 c 504 g 343 t
ORIGIN
1 gggctgtcgc ggcttgagga ggggcggggc ccaaggggcg ggacctcggg tggcccgagg
61 tggcccggct gctgggttgg aagcggcgca gtccgactca tcccggcccc gggatggcgt
121 ccccacggga attgacacag aaccccctga agaagatctg gatgccatac agcaatgggc
181 ggcccgctct gcacgcttgc cagcgcggtg tgtgcatgac caactgccca actctcattg
241 tcatggtggg cctgcccgcc aggggcaaga cctacatctc caagaagctg actcgatacc
301 tgaactggat tggtgtgccc actcgggagt tcaatgttgg ccagtatcgc cgggacgtgg
361 tcaagaccta caaatctttt gaattttttc tccccgacaa tgaagagggc ctgaaaatca
421 ggaaatgagg aaactgaggc tcagacaggc caggagtctc gcacaaaata gcaagtttac
481 atcagcgccc agacttgaat ctaagcccat ccaccgctaa aaagtgtcca tcattccaat
541 attcctcaat aggcagtgtg ccctggcagc cctccgtgac gtccggcggt tccttagtga
601 ggagggggga catgtggcgg tttttgatgc cacaaacacc acccgagaac ggagagcgac
661 catctttaat tttggagaac agaatggcta caagaccttt tttgtcgagt ccatctgtgt
721 ggatcctgag gtcatagctg ccaacatcgt gcaagtgaaa ctgggcagcc ctgactatgt
781 caaccgcgac agtgatgagg ctacggagga cttcatgagg cgcattgagt gctatgagaa
841 ctcctacgag tcgctagatg aggacctgga tagggacctg tcctatatca agatcatgga
901 tgtgggccag agctacgtgg tgaaccgtgt ggctgaccac atccagagcc gcatcgtata
961 ttacctcatg aacatccacg tgaccccccg ctccatctac ctctgccggc acggggagag
1021 cgagctcaac ctcaagggcc ggattggcgg ggacccagga ctgtcccctc ggggcaggga
1081 gtttgccaag agtctagccc agttcatcag tgaccaaaat atcaaggatc tgaaggtctg
1141 gacaagccag atgaagagga caatccagac ggctgaggca ctgggtgtgc cctatgaaca
1201 gtggaaggtc ctcaacgaga tcgatgcggg cgtctgtgag gaaatgacct acgaggaaat
1261 tcaggataat tatccactgg agttcgccct gcgggaccag gacaagtacc ggtaccggta
1321 ccctaaaggg gagtcctacg aggacctggt ccagagactg gagcctgtca tcatggagct
1381 ggagaggcaa gagaatgtgc tggtcatctg ccaccaggct gtgatgcgct gcctgctggc
1441 ctacttcctc gacaaggcag cagaacagct gccctacctc aagtgtccgc tgcacacagt
1501 cctgaagctg actcctgtgg catatggttg taaagtggag tccatattcc tgaacgtggc
1561 tgctgtgaac acgcaccggg acaggcctca gaacgtggac atctcaagac ctccagagga
1621 agcccttgtc acggtgcctg ctcaccagtg accatgttca tccactgtga ccactaggca
1681 ggcactgctc tctgcagagg gggtcattcc aggccctcca gtgt
//