LOCUS AY424270 5776 bp mRNA linear HUM 23-DEC-2004
DEFINITION Homo sapiens myosin lignt chain polypeptide kinase isoform 1 (MYLK)
mRNA, complete cds, alternatively spliced.
ACCESSION AY424270
VERSION AY424270.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5776)
AUTHORS Clayburgh,D.R., Rosen,S., Witkowski,E.D., Wang,F., Blair,S.,
Dudek,S., Garcia,J.G., Alverdy,J.C. and Turner,J.R.
TITLE A differentiation-dependent splice variant of myosin light chain
kinase, MLCK1, regulates epithelial tight junction permeability
JOURNAL J. Biol. Chem. 279 (53), 55506-55513 (2004)
PUBMED 15507455
REFERENCE 2 (bases 1 to 5776)
AUTHORS Clayburgh,D.R. and Turner,J.R.
TITLE Direct Submission
JOURNAL Submitted (29-SEP-2003) Pathology, The University of Chicago, 5841
S. Maryland Ave., MC 1089, Chicago, IL 60637, USA
FEATURES Location/Qualifiers
source 1..5776
/db_xref="H-InvDB:HIT000254530"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3q21"
/cell_line="Caco-2 BBe 5E6L"
/note="intestinal epithelial cell line"
gene 1..5776
/gene="MYLK"
CDS 3..5747
/gene="MYLK"
/note="alternatively spliced"
/codon_start=1
/product="myosin lignt chain polypeptide kinase isoform 1"
/protein_id="AAR29062.1"
/translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECP
PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEVSGIPKPEVAWFLE
GTPVRRQEGSIEVYENAGSHYLCLLKARTRDSGTYSCTASNAQGQVSCSWTLQVERLA
VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVA
ELHIQDALPEDHGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPI
FLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLCI
QEVFPEDTGTYTCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLI
SCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKN
RVGECSCQVSLMLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPAR
GQGWLEEEDGEDVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDD
LKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVP
PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAK
PAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNE
KRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIIL
SQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSR
RPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVT
GTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQ
AQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTW
KELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVE
VSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKF
FKAYSAKEKENIRQEISIMNCLHPPKLVQCVDAFEEKANIVMVLEIVSGGELFERIID
EDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLAR
RLENAGSLKVLFGTPEFVAPEVIIYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNE
TLANVTSPTWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNM
EAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAE
KLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVW
FKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVE
TMEEGEGEGEEEEE"
BASE COUNT 1500 a 1538 c 1677 g 1061 t
ORIGIN
1 ccatggggga tgtgaagctg gttgcctcgt cacacatttc caaaacctcc ctcagtgtgg
61 atccctcaag agttgactcc atgcccctga cagaggcccc tgctttcatt ttgccccctc
121 ggaacctctg catcaaagaa ggagccaccg ccaagttcga agggcgggtc cggggttacc
181 cagagcccca ggtgacatgg cacagaaacg ggcaacccat caccagcggg ggccgcttcc
241 tgctggattg cggcatccgg gggactttca gccttgtgat tcatgctgtc catgaggagg
301 acaggggaaa gtatacctgt gaagccacca atggcagtgg tgctcgccag gtgacagtgg
361 agttgacagt agaaggaagt tttgcgaagc agcttggtca gcctgttgtt tccaaaacct
421 taggggatag attttcagct tcagcagtgg agacccgtcc tagcatctgg ggggagtgcc
481 caccaaagtt tgctaccaag ctgggccgag ttgtggtcaa agaaggacag atgggacgat
541 tctcctgcaa gatcactggc cggccccaac cgcaggtcac ctggctcaag ggaaatgttc
601 cactgcagcc gagtgcccgt gtgtctgtgt ctgagaagaa cggcatgcag gttctggaaa
661 tccatggagt caaccaagat gacgtgggag tgtacacgtg cctggtggtg aacgggtcgg
721 ggaaggcctc gatgtcagct gaactttcca tccaaggttt ggacagtgcc aataggtcat
781 ttgtgagaga aacaaaagcc accaattcag atgtcaggaa agaggtgacc aatgtaatct
841 caaaggagtc gaagctggac agtctggagg ctgcagccaa aagcaagaac tgctccagcc
901 cccagagagg tggctcccca ccctgggctg caaacagcca gcctcagccc ccaagggagt
961 ccaagctgga gtcatgcaag gactcgccca gaacggcccc gcagaccccg gtccttcaga
1021 agacttccag ctccatcacc ctgcaggccg caagagttca gccggaacca agagcaccag
1081 gcctgggggt cctatcacct tctggagaag agaggaagag gccagctcct ccccgtccag
1141 ccaccttccc caccaggcag cctggcctgg ggagccaaga tgttgtgagc aaggctgcta
1201 acaggagaat ccccatggag ggccagaggg attcagcatt ccccaaattt gagagcaagc
1261 cccaaagcca ggaggtcaag gaaaatcaaa ctgtcaagtt cagatgtgaa gtttccggga
1321 ttccaaagcc tgaagtggcc tggttcctgg aaggcacccc cgtgaggaga caggaaggca
1381 gcattgaggt ttatgaaaat gctggctccc attacctctg cctgctgaaa gcccggacca
1441 gggacagtgg gacatacagc tgcactgctt ccaacgccca aggccaggtg tcctgtagct
1501 ggaccctcca agtggaaagg cttgccgtga tggaggtggc cccctccttc tccagtgtcc
1561 tgaaggactg cgctgttatt gagggccagg attttgtgct gcagtgctcc gtacggggga
1621 ccccagtgcc ccggatcact tggctgctga atgggcagcc catccagtac gctcgctcca
1681 cctgcgaggc cggcgtggct gagctccaca tccaggatgc cctgccggag gaccatggca
1741 cctacacctg cctagctgag aatgccttgg ggcaggtgtc ctgcagcgcc tgggtcaccg
1801 tccatgaaaa gaagagtagc aggaagagtg agtaccttct gcctgtggct cccagcaagc
1861 ccactgcacc catcttcctg cagggcctct ctgatctcaa agtcatggat ggaagccagg
1921 tcactatgac tgtccaagtg tcagggaatc caccccctga agtcatctgg ctgcacaatg
1981 ggaatgagat ccaagagtca gaggacttcc actttgaaca gagaggaact cagcacagcc
2041 tttgtatcca ggaagtgttc ccggaggaca cgggcacgta cacctgcgag gcctggaaca
2101 gcgctggaga ggtccgcacc caggccgtgc tcacggtaca agagcctcac gatggcaccc
2161 agccctggtt catcagtaag cctcgctcag tgacagcctc cctgggccag agtgtcctca
2221 tctcctgcgc catagctggt gacccctttc ctaccgtgca ctggctcaga gatggcaaag
2281 ccctctgcaa agacactggc cacttcgagg tgcttcagaa tgaggacgtg ttcaccctgg
2341 ttctaaagaa ggtgcagccc tggcatgccg gccagtatga gatcctgctc aagaaccggg
2401 ttggcgaatg cagttgccag gtgtcactga tgctacagaa cagctctgcc agagcccttc
2461 cacgggggag ggagcctgcc agctgcgagg acctctgtgg tggaggagtt ggtgctgatg
2521 gtggtggtag tgaccgctat gggtccctga ggcctggctg gccagcaaga gggcagggtt
2581 ggctagagga ggaagacggc gaggacgtgc gaggggtgct gaagaggcgc gtggagacga
2641 ggcagcacac tgaggaggcg atccgccagc aggaggtgga gcagctggac ttccgagacc
2701 tcctggggaa gaaggtgagt acaaagaccc tatcggaaga cgacctgaag gagatcccag
2761 ccgagcagat ggatttccgt gccaacctgc aacggcaagt gaagccaaag actgtgtctg
2821 aggaagagag gaaggtgcac agcccccagc aggtcgattt tcgctctgtc ctggccaaga
2881 aggggacttc caagaccccc gtgcctgaga aggtgccacc gccaaaacct gccaccccgg
2941 attttcgctc agtgctgggt ggcaagaaga aattaccagc agagaatggc agcagcagtg
3001 ccgagaccct gaatgccaag gcagtggaga gttccaagcc cctgagcaat gcacagcctt
3061 cagggccctt gaaacccgtg ggcaacgcca agcctgctga gaccctgaag ccaatgggca
3121 acgccaagcc tgccgagacc ctgaagccca tgggcaatgc caagcctgat gagaacctga
3181 aatccgctag caaagaagaa ctcaagaaag acgttaagaa tgatgtgaac tgcaagagag
3241 gccatgcagg gaccacagat aatgaaaaga gatcagagag ccaggggaca gccccagcct
3301 tcaagcagaa gctgcaagat gttcatgtgg cagagggcaa gaagctgctg ctccagtgcc
3361 aggtgtcttc tgacccccca gccaccatca tctggacgct gaacggaaag accctcaaga
3421 ccaccaagtt catcatcctc tcccaggaag gctcactctg ctccgtctcc atcgagaagg
3481 cactgcctga ggacagaggc ttatacaagt gtgtagccaa gaatgacgct ggccaggcgg
3541 agtgctcctg ccaagtcact gtggatgatg ctccagccag tgagaacacc aaggccccag
3601 agatgaaatc ccggaggccc aagagctctc ttcctcccgt gctaggaact gagagtgatg
3661 cgactgtgaa aaagaaacct gcccccaaga cacctccgaa ggcagcaatg ccccctcaga
3721 tcatccagtt ccctgaggac cagaaggtac gcgcaggaga gtcagtggag ctgtttggca
3781 aagtgacagg cactcagccc atcacctgta cctggatgaa gttccgaaag cagatccagg
3841 aaagcgagca catgaaggtg gagaacagcg agaatggcag caagctcacc atcctggccg
3901 cgcgccagga gcactgcggc tgctacacac tgctggtgga gaacaagctg ggcagcaggc
3961 aggcccaggt caacctcact gtcgtggata agccagaccc cccagctggc acaccttgtg
4021 cctctgacat tcggagctcc tcactgaccc tgtcctggta tggctcctca tatgatgggg
4081 gcagtgctgt acagtcctac agcatcgaga tctgggactc agccaacaag acgtggaagg
4141 aactagccac atgccgcagc acctctttca acgtccagga cctgctgcct gaccacgaat
4201 ataagttccg tgtacgtgca atcaacgtgt atggaaccag tgagccaagc caggagtctg
4261 aactcacaac ggtaggagag aaacctgaag agccgaagga tgaagtggag gtgtcagatg
4321 atgatgagaa ggagcccgag gttgattacc ggacagtgac aatcaatact gaacaaaaag
4381 tatctgactt ctacgacatt gaggagagat taggatctgg gaaatttgga caggtctttc
4441 gacttgtaga aaagaaaact cgaaaagtct gggcagggaa gttcttcaag gcatattcag
4501 caaaagagaa agagaatatc cggcaggaga ttagcatcat gaactgcctc caccccccta
4561 agctggtcca gtgtgtggat gcctttgaag aaaaggccaa catcgtcatg gtcctggaga
4621 tcgtgtcagg aggggagctg tttgagcgca tcattgacga ggactttgag ctgacggagc
4681 gtgagtgcat caagtacatg cggcagatct cggagggagt ggagtacatc cacaagcagg
4741 gcatcgtgca cctggacctc aagccggaga acatcatgtg tgtcaacaag acgggcacca
4801 ggatcaagct catcgacttt ggtctggcca ggaggctgga gaatgcgggg tctctgaagg
4861 tcctctttgg caccccagaa tttgtggctc ctgaagtgat catctatgag cccatcggct
4921 acgccacaga catgtggagc atcggggtca tctgctacat cctagtcagt ggcctttccc
4981 ccttcatggg agacaacgat aacgaaacct tggccaacgt tacctcaccc acctgggact
5041 tcgacgacga ggcattcgat gagatctccg acgatgccaa ggatttcatc agcaatctgc
5101 tgaagaaaga tatgaaaaac cgcctggact gcacgcagtg ccttcagcat ccatggctaa
5161 tgaaagatac caagaacatg gaggccaaga aactctccaa ggaccggatg aagaagtaca
5221 tggcaagaag gaaatggcag aaaacgggca atgctgtgag agccattgga agactgtcct
5281 ctatggcaat gatctcaggg ctcagtggca ggaaatcctc aacagggtca ccaaccagcc
5341 cgctcaatgc agaaaaacta gaatctgaag aagatgtgtc ccaagctttc cttgaggctg
5401 ttgctgagga aaagcctcat gtaaaaccct atttctctaa gaccattcgc gatttagaag
5461 ttgtggaggg aagtgctgct agatttgact gcaagattga aggataccca gaccccgagg
5521 ttgtctggtt caaagatgac cagtcaatca gggagtcccg ccacttccag atagactacg
5581 atgaggacgg gaactgctct ttaattatta gtgatgtttg cggggatgac gatgccaagt
5641 acacctgcaa ggctgtcaac agtcttggag aagccacctg cacagcagag ctcattgtgg
5701 aaacgatgga ggaaggtgaa ggggaagggg aagaggaaga agagtgaaac aaagccagag
5761 aaaagcagtt tctaag
//