LOCUS AY424269 5569 bp mRNA linear HUM 23-DEC-2004
DEFINITION Homo sapiens myosin light chain polypeptide kinase isoform 2 (MYLK)
mRNA, complete cds, alternatively spliced.
ACCESSION AY424269
VERSION AY424269.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5569)
AUTHORS Clayburgh,D.R., Rosen,S., Witkowski,E.D., Wang,F., Blair,S.,
Dudek,S., Garcia,J.G., Alverdy,J.C. and Turner,J.R.
TITLE A differentiation-dependent splice variant of myosin light chain
kinase, MLCK1, regulates epithelial tight junction permeability
JOURNAL J. Biol. Chem. 279 (53), 55506-55513 (2004)
PUBMED 15507455
REFERENCE 2 (bases 1 to 5569)
AUTHORS Clayburgh,D.R. and Turner,J.R.
TITLE Direct Submission
JOURNAL Submitted (29-SEP-2003) Pathology, The University of Chicago, 5841
S. Maryland Ave., MC 1089, Chicago, IL 60637, USA
FEATURES Location/Qualifiers
source 1..5569
/db_xref="H-InvDB:HIT000254529"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3q21"
/cell_line="Caco-2 BBe 5E6L"
/note="intestinal epithelial cell line"
gene 1..5569
/gene="MYLK"
CDS 3..5540
/gene="MYLK"
/note="alternatively spliced"
/codon_start=1
/product="myosin light chain polypeptide kinase isoform 2"
/protein_id="AAR29061.1"
/translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECP
PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEGLAVMEVAPSFSSV
LKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVAELHIQDALPED
HGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPIFLQGLSDLKVM
DGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLCIQEVFPEDTGTY
TCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPT
VHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQVSL
MLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPARGQGWLEEEDGE
DVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDDLKEIPAEQMDF
RANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPKPATPDFRS
VLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNA
KPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTAPA
FKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSI
EKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLG
TESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK
FRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKP
DPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTWKELATCRSTSF
NVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEV
DYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKEN
IRQEISIMNCLHPPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECI
KYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVL
FGTPEFVAPEVIIYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSPTWD
FDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMK
KYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQA
FLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESR
HFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEE
EEE"
BASE COUNT 1451 a 1480 c 1617 g 1021 t
ORIGIN
1 ccatggggga tgtgaagctg gttgcctcgt cacacatttc caaaacctcc ctcagtgtgg
61 atccctcaag agttgactcc atgcccctga cagaggcccc tgctttcatt ttgccccctc
121 ggaacctctg catcaaagaa ggagccaccg ccaagttcga agggcgggtc cggggttacc
181 cagagcccca ggtgacatgg cacagaaacg ggcaacccat caccagcggg ggccgcttcc
241 tgctggattg cggcatccgg gggactttca gccttgtgat tcatgctgtc catgaggagg
301 acaggggaaa gtatacctgt gaagccacca atggcagtgg tgctcgccag gtgacagtgg
361 agttgacagt agaaggaagt tttgcgaagc agcttggtca gcctgttgtt tccaaaacct
421 taggggatag attttcagct tcagcagtgg agacccgtcc tagcatctgg ggggagtgcc
481 caccaaagtt tgctaccaag ctgggccgag ttgtggtcaa agaaggacag atgggacgat
541 tctcctgcaa gatcactggc cggccccaac cgcaggtcac ctggctcaag ggaaatgttc
601 cactgcagcc gagtgcccgt gtgtctgtgt ctgagaagaa cggcatgcag gttctggaaa
661 tccatggagt caaccaagat gacgtgggag tgtacacgtg cctggtggtg aacgggtcgg
721 ggaaggcctc gatgtcagct gaactttcca tccaaggttt ggacagtgcc aataggtcat
781 ttgtgagaga aacaaaagcc accaattcag atgtcaggaa agaggtgacc aatgtaatct
841 caaaggagtc gaagctggac agtctggagg ctgcagccaa aagcaagaac tgctccagcc
901 cccagagagg tggctcccca ccctgggctg caaacagcca gcctcagccc ccaagggagt
961 ccaagctgga gtcatgcaag gactcgccca gaacggcccc gcagaccccg gtccttcaga
1021 agacttccag ctccatcacc ctgcaggccg caagagttca gccggaacca agagcaccag
1081 gcctgggggt cctatcacct tctggagaag agaggaagag gccagctcct ccccgtccag
1141 ccaccttccc caccaggcag cctggcctgg ggagccaaga tgttgtgagc aaggctgcta
1201 acaggagaat ccccatggag ggccagaggg attcagcatt ccccaaattt gagagcaagc
1261 cccaaagcca ggaggtcaag gaaaatcaaa ctgtcaagtt cagatgtgaa gggcttgccg
1321 tgatggaggt ggccccctcc ttctccagtg tcctgaagga ctgcgctgtt attgagggcc
1381 aggattttgt gctgcagtgc tccgtacggg ggaccccagt gccccggatc acttggctgc
1441 tgaatgggca gcccatccag tacgctcgct ccacctgcga ggccggcgtg gctgagctcc
1501 acatccagga tgccctgccg gaggaccatg gcacctacac ctgcctagct gagaatgcct
1561 tggggcaggt gtcctgcagc gcctgggtca ccgtccatga aaagaagagt agcaggaaga
1621 gtgagtacct tctgcctgtg gctcccagca agcccactgc acccatcttc ctgcagggcc
1681 tctctgatct caaagtcatg gatggaagcc aggtcactat gactgtccaa gtgtcaggga
1741 atccaccccc tgaagtcatc tggctgcaca atgggaatga gatccaagag tcagaggact
1801 tccactttga acagagagga actcagcaca gcctttgtat ccaggaagtg ttcccggagg
1861 acacgggcac gtacacctgc gaggcctgga acagcgctgg agaggtccgc acccaggccg
1921 tgctcacggt acaagagcct cacgatggca cccagccctg gttcatcagt aagcctcgct
1981 cagtgacagc ctccctgggc cagagtgtcc tcatctcctg cgccatagct ggtgacccct
2041 ttcctaccgt gcactggctc agagatggca aagccctctg caaagacact ggccacttcg
2101 aggtgcttca gaatgaggac gtgttcaccc tggttctaaa gaaggtgcag ccctggcatg
2161 ccggccagta tgagatcctg ctcaagaacc gggttggcga atgcagttgc caggtgtcac
2221 tgatgctaca gaacagctct gccagagccc ttccacgggg gagggagcct gccagctgcg
2281 aggacctctg tggtggagga gttggtgctg atggtggtgg tagtgaccgc tatgggtccc
2341 tgaggcctgg ctggccagca agagggcagg gttggctaga ggaggaagac ggcgaggacg
2401 tgcgaggggt gctgaagagg cgcgtggaga cgaggcagca cactgaggag gcgatccgcc
2461 agcaggaggt ggagcagctg gacttccgag acctcctggg gaagaaggtg agtacaaaga
2521 ccctatcgga agacgacctg aaggagatcc cagccgagca gatggatttc cgtgccaacc
2581 tgcaacggca agtgaagcca aagactgtgt ctgaggaaga gaggaaggtg cacagccccc
2641 agcaggtcga ttttcgctct gtcctggcca agaaggggac ttccaagacc cccgtgcctg
2701 agaaggtgcc accgccaaaa cctgccaccc cggattttcg ctcagtgctg ggtggcaaga
2761 agaaattacc agcagagaat ggcagcagca gtgccgagac cctgaatgcc aaggcagtgg
2821 agagttccaa gcccctgagc aatgcacagc cttcagggcc cttgaaaccc gtgggcaacg
2881 ccaagcctgc tgagaccctg aagccaatgg gcaacgccaa gcctgccgag accctgaagc
2941 ccatgggcaa tgccaagcct gatgagaacc tgaaatccgc tagcaaagaa gaactcaaga
3001 aagacgttaa gaatgatgtg aactgcaaga gaggccatgc agggaccaca gataatgaaa
3061 agagatcaga gagccagggg acagccccag ccttcaagca gaagctgcaa gatgttcatg
3121 tggcagaggg caagaagctg ctgctccagt gccaggtgtc ttctgacccc ccagccacca
3181 tcatctggac gctgaacgga aagaccctca agaccaccaa gttcatcatc ctctcccagg
3241 aaggctcact ctgctccgtc tccatcgaga aggcactgcc tgaggacaga ggcttataca
3301 agtgtgtagc caagaatgac gctggccagg cggagtgctc ctgccaagtc actgtggatg
3361 atgctccagc cagtgagaac accaaggccc cagagatgaa atcccggagg cccaagagct
3421 ctcttcctcc cgtgctagga actgagagtg atgcgactgt gaaaaagaaa cctgccccca
3481 agacacctcc gaaggcagca atgccccctc agatcatcca gttccctgag gaccagaagg
3541 tacgcgcagg agagtcagtg gagctgtttg gcaaagtgac aggcactcag cccatcacct
3601 gtacctggat gaagttccga aagcagatcc aggaaagcga gcacatgaag gtggagaaca
3661 gcgagaatgg cagcaagctc accatcctgg ccgcgcgcca ggagcactgc ggctgctaca
3721 cactgctggt ggagaacaag ctgggcagca ggcaggccca ggtcaacctc actgtcgtgg
3781 ataagccaga ccccccagct ggcacacctt gtgcctctga cattcggagc tcctcactga
3841 ccctgtcctg gtatggctcc tcatatgatg ggggcagtgc tgtacagtcc tacagcatcg
3901 agatctggga ctcagccaac aagacgtgga aggaactagc cacatgccgc agcacctctt
3961 tcaacgtcca ggacctgctg cctgaccacg aatataagtt ccgtgtacgt gcaatcaacg
4021 tgtatggaac cagtgagcca agccaggagt ctgaactcac aacggtagga gagaaacctg
4081 aagagccgaa ggatgaagtg gaggtgtcag atgatgatga gaaggagccc gaggttgatt
4141 accggacagt gacaatcaat actgaacaaa aagtatctga cttctacgac attgaggaga
4201 gattaggatc tgggaaattt ggacaggtct ttcgacttgt agaaaagaaa actcgaaaag
4261 tctgggcagg gaagttcttc aaggcatatt cagcaaaaga gaaagagaat atccggcagg
4321 agattagcat catgaactgc ctccaccccc ctaagctggt ccagtgtgtg gatgcctttg
4381 aagaaaaggc caacatcgtc atggtcctgg agatcgtgtc aggaggggag ctgtttgagc
4441 gcatcattga cgaggacttt gagctgacgg agcgtgagtg catcaagtac atgcggcaga
4501 tctcggaggg agtggagtac atccacaagc agggcatcgt gcacctggac ctcaagccgg
4561 agaacatcat gtgtgtcaac aagacgggca ccaggatcaa gctcatcgac tttggtctgg
4621 ccaggaggct ggagaatgcg gggtctctga aggtcctctt tggcacccca gaatttgtgg
4681 ctcctgaagt gatcatctat gagcccatcg gctacgccac agacatgtgg agcatcgggg
4741 tcatctgcta catcctagtc agtggccttt cccccttcat gggagacaac gataacgaaa
4801 ccttggccaa cgttacctca cccacctggg acttcgacga cgaggcattc gatgagatct
4861 ccgacgatgc caaggatttc atcagcaatc tgctgaagaa agatatgaaa aaccgcctgg
4921 actgcacgca gtgccttcag catccatggc taatgaaaga taccaagaac atggaggcca
4981 agaaactctc caaggaccgg atgaagaagt acatggcaag aaggaaatgg cagaaaacgg
5041 gcaatgctgt gagagccatt ggaagactgt cctctatggc aatgatctca gggctcagtg
5101 gcaggaaatc ctcaacaggg tcaccaacca gcccgctcaa tgcagaaaaa ctagaatctg
5161 aagaagatgt gtcccaagct ttccttgagg ctgttgctga ggaaaagcct catgtaaaac
5221 cctatttctc taagaccatt cgcgatttag aagttgtgga gggaagtgct gctagatttg
5281 actgcaagat tgaaggatac ccagaccccg aggttgtctg gttcaaagat gaccagtcaa
5341 tcagggagtc ccgccacttc cagatagact acgatgagga cgggaactgc tctttaatta
5401 ttagtgatgt ttgcggggat gacgatgcca agtacacctg caaggctgtc aacagtcttg
5461 gagaagccac ctgcacagca gagctcattg tggaaacgat ggaggaaggt gaaggggaag
5521 gggaagagga agaagagtga aacaaagcca gagaaaagca gtttctaag
//