LOCUS AW665944 540 bp mRNA linear EST 08-JAN-2011 DEFINITION hj10b09.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:2981369 3' similar to TR:Q62845 Q62845 NEURAL CELL ADHESION PROTEIN BIG-2 PRECURSOR, mRNA sequence. ACCESSION AW665944 VERSION AW665944.1 DBLINK BioSample: SAMN00155629 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 540) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40UP from Gibco High quality sequence stop: 314. FEATURES Location/Qualifiers source 1..540 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2981369" /clone_lib="SAMN00155629 Soares_NFL_T_GBC_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo." BASE COUNT 145 a 104 c 106 g 185 t ORIGIN 1 ttttttaaag aagcaaaaaa catttttatt ccagtaataa gatgtacttg aacaacaata 61 ttacttagta ctgggtgtct tcaaaacaac tagctaaatg ttgcttatat tataaacagc 121 aagtccttca gataactttt gtcataaact ggacctagct gtgagggaaa tcattattgt 181 actgatggct gacagcgtac atgcattcga agtggaagcc ccagatcctt tcgcgtaggc 241 atttgatatc tttggaattc gaatttgttc actgctgctg ccatctcctc cggcgctgaa 301 tggcttaatt tctattatat aatcttcatc gaagggcaaa gaaagctcca ccgatgtttt 361 atttgtttca atgacaaatg tgctgcttat gctgttccat ctgtacaaaa ctttgtatcc 421 ttttacttcc gactcattat ccagggcctt cacttgatcc caattcaggg atattttgga 481 gttctatgaa ttccatatta tagtttccgg gggttgactt gggggtggct ttcgggtgga //