LOCUS       AW104394                 438 bp    mRNA    linear   EST 04-JAN-2011
DEFINITION  xd77g10.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone
            IMAGE:2603682 3', mRNA sequence.
ACCESSION   AW104394
VERSION     AW104394.1
DBLINK      BioSample: SAMN00155629
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 438)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -40UP from Gibco
            High quality sequence stop: 434.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2603682"
                     /clone_lib="SAMN00155629 Soares_NFL_T_GBC_S1"
                     /lab_host="DH10B"
                     /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Equal amounts of plasmid DNA from three
                     normalized libraries (fetal lung NbHL19W, testis NHT, and
                     B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made
                     in vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from pools of 5,000 clones made
                     from the same 3 libraries. The pools consisted of
                     I.M.A.G.E. clones 297480-302087, 682632-687239,
                     726408-728711, and 729096-731399. Subtraction by Bento
                     Soares and M. Fatima Bonaldo."
BASE COUNT          102 a          103 c           89 g          144 t
ORIGIN      
        1 ttttacttcc agacatattt attcagtagc ttcttaaatt catcagggaa atgctttttc
       61 cactttagct ctactctgcc atcctcacca tgttaagttg aatctctcct catgtcacca
      121 ggtggcctct gcagtctggc atcactccag gtaccacagc atcctgtagt tactctgtct
      181 tctaagagct ggtgtagggg tggtagacag tgatgtagaa ggtggtcaga atcacagctt
      241 tcatgccagt gggagaataa atctctatac tgctttcttt tgcctgggac caatctgaag
      301 tgcctgttgt gttctgcagt atcataagtc gccagggtct ctctgtctgg catagccatt
      361 ctcattccca aagtgcttca gggatttgga tcaaatccaa agactattct gcttgttcat
      421 tctgaacaga ttaattca
//