LOCUS AW104394 438 bp mRNA linear EST 04-JAN-2011 DEFINITION xd77g10.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:2603682 3', mRNA sequence. ACCESSION AW104394 VERSION AW104394.1 DBLINK BioSample: SAMN00155629 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 438) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40UP from Gibco High quality sequence stop: 434. FEATURES Location/Qualifiers source 1..438 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2603682" /clone_lib="SAMN00155629 Soares_NFL_T_GBC_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo." BASE COUNT 102 a 103 c 89 g 144 t ORIGIN 1 ttttacttcc agacatattt attcagtagc ttcttaaatt catcagggaa atgctttttc 61 cactttagct ctactctgcc atcctcacca tgttaagttg aatctctcct catgtcacca 121 ggtggcctct gcagtctggc atcactccag gtaccacagc atcctgtagt tactctgtct 181 tctaagagct ggtgtagggg tggtagacag tgatgtagaa ggtggtcaga atcacagctt 241 tcatgccagt gggagaataa atctctatac tgctttcttt tgcctgggac caatctgaag 301 tgcctgttgt gttctgcagt atcataagtc gccagggtct ctctgtctgg catagccatt 361 ctcattccca aagtgcttca gggatttgga tcaaatccaa agactattct gcttgttcat 421 tctgaacaga ttaattca //