LOCUS AL023657 2530 bp mRNA linear HUM 15-OCT-2008
DEFINITION Homo sapiens SH2D1A cDNA, formerly known as DSHP.
ACCESSION AL023657
VERSION AL023657.1
KEYWORDS SH2D1A.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2530)
AUTHORS Coffey A.J., Brooksbank R.A.
JOURNAL Submitted (21-MAY-1998) to the INSDC. E-mail contact:
humquery@sanger.ac.uk Clone requests:clonerequest@sanger.ac.uk
COMMENT This sequence was generated from a cDNA clone isolated using
sequence from the bacterial clone dJ1052M9
To amplify the coding sequence with flanking intronic or
untranslated
sequence from genomic DNA the following primer pairs are used. Exon
1
is amplified using two rounds of PCR in round 1 the forward primer
is
GTTGAGCTAAGTTATTCCTG and the reverse primer is
TGAGGCGAAAGTGTGTTCCA,
the product of this reaction is amplified with ACAGAAGCATTACTAAGC
and
CCAGCTGCTGTTGCCCAC. Exon 2, forward primer CAATGACACCATATACGTGT,
reverse
primer GCTTTCTTAATGATCCATGA. Exon 3, forward primer
CAAGTTACACAAATGTTA,
reverse primer CTTGGACTCATAACTCTG. Exon 4 forward primer
TCATTGTGAGTTTTATGCAG, reverse primer GCTCACCGAACTGTATTA.
FEATURES Location/Qualifiers
source 1..2530
/db_xref="H-InvDB:HIT000250035"
/organism="Homo sapiens"
/chromosome="X"
/map="q25"
/mol_type="mRNA"
/note="locus SH2D1A"
/db_xref="taxon:9606"
exon 1..436
/gene="SH2D1A"
/number=1
mRNA join(1..436,437..500,501..645,646..2530)
/gene="SH2D1A"
/product="SH2D1A"
CDS 300..686
/gene="SH2D1A"
/product="SH2D1A"
/db_xref="GOA:O60880"
/db_xref="H-InvDB:HIT000250035.14"
/db_xref="HGNC:HGNC:10820"
/db_xref="InterPro:IPR000980"
/db_xref="InterPro:IPR017289"
/db_xref="InterPro:IPR035876"
/db_xref="InterPro:IPR036860"
/db_xref="PDB:1D1Z"
/db_xref="PDB:1D4T"
/db_xref="PDB:1D4W"
/db_xref="PDB:1KA6"
/db_xref="PDB:1KA7"
/db_xref="PDB:1M27"
/db_xref="UniProtKB/Swiss-Prot:O60880"
/protein_id="CAA19222.1"
/translation="MDAVAVYHGKISRETGEKLLLATGLDGSYLLRDSESVPGVYCLC
VLYHGYIYTYRVSQTETGSWSAETAPGVHKRYFRKIKNLISAFQKPDQGIVIPLQYPV
EKKSSARSTQGTTGIREDPDVCLKAP"
exon 437..500
/gene="SH2D1A"
/number=2
exon 501..645
/gene="SH2D1A"
/number=3
exon 646..2530
/gene="SH2D1A"
/number=4
BASE COUNT 837 a 419 c 517 g 757 t
ORIGIN
1 aaatccttct tccaatgttc ctcccctctc tgtatgaacc ctgtgttggg gggcagaaga
61 tggaagccct tggcaagctc gatcgaacca agctactaaa ttgctgagct cgttttaact
121 gaagtgtgag aaggaggttt aaggcaagta gacaacatcc tgttgttggg gtgcttctct
181 cttttttgca catctggctg aactgggagt caggtggttg acttgtgcct ggctgcagta
241 gcagcggcat ctcccttgca cagttctcct cctcggcctg cccaagagtc caccaggcca
301 tggacgcagt ggctgtgtat catggcaaaa tcagcaggga aaccggcgag aagctcctgc
361 ttgccactgg gctggatggc agctatttgc tgagggacag cgagagcgtg ccaggcgtgt
421 actgcctatg tgtgctgtat cacggttaca tttatacata ccgagtgtcc cagacagaaa
481 caggttcttg gagtgctgag acagcacctg gggtacataa aagatatttc cggaaaataa
541 aaaatctcat ttcagcattt cagaagccag atcaaggcat tgtaatacct ctgcagtatc
601 cagttgagaa gaagtcctca gctagaagta cacaaggtac tacagggata agagaagatc
661 ctgatgtctg cctgaaagcc ccatgaagaa aaataaaaca ccttgtactt tattttctat
721 aatttaaata tatgctaagt cttatatatt gtagataata cagttcggtg agctacaaat
781 gcatttctaa agccattgta gtcctgtaat ggaagcatct agcatgtcgt caaagctgaa
841 atggactttt gtacatagtg aggagctttg aaacgaggat tgggaaaaag taattccgta
901 ggttattttc agttattata tttacaaatg ggaaacaaaa ggataatgaa tactttataa
961 aggattaatg tcaattcttg ccaaatataa ataaaaataa tcctcagttt ttgtgaaaag
1021 ctccattttt agtgaaatat tattttatag ctactaattt taaaatgtct tgcttgattg
1081 tatggtggga agttggctgg tgtcccttgt ctttgccaag ttctccacta gctatggtgt
1141 cataggctct tttgggattt ttgaagctgt atactgtgtg ctaaaacaag cactaaacaa
1201 agagtgaagg atttatgttt aattctgaaa gcaaccttct tgcctagtgt tctgatattg
1261 gacagtaaaa tccacagacc aacctggagt tgaaaatctt ataatttaaa atatgctcta
1321 aacatgttta tcgtatttga tgctacagga tttgaaattg tattacaaat ccaatgaaat
1381 gagtttttct tttcatttac ctctgcccca gttgtttcta ctacatggaa gacctcattt
1441 tgaagggaaa tttcagcagc tgcagctcat gagtaactga tttgtaacaa gcctcctttt
1501 aaagtaaccc tacaaaacca ctggaaagtt tatggttgta ttatttttta aaaaaattcc
1561 aagtgattga aacctacacg agatacagaa ttttatgcgg cattttcttc tcacatttat
1621 atttttgtga ttttgtgatt gattatatgt cactttgcta cagggctcac agaattcatt
1681 cactcaacaa acataatagg gcgctgaggg catagaagta aaaacacctg gtccctgctc
1741 tcagttcact gtcttgttgg acgagaaaag aaacaataac gataaaagac agtgaaagaa
1801 aataacgata aaagacagtg aaagaaaata acaataaaag acaaggaaaa aataacaatg
1861 aaagttgata agtacatgat aagcgaggtt ccccgtgtgt aggtagatct ggtctttaga
1921 ggcagataga taggtcagtg caaatactct ggtccatggg ccatatgaaa aggctaagct
1981 tcactgtaaa ataataactg ggaattctgg attgtgtatg ggtgttggtg aacttggttt
2041 taattagtga actgctgaga gacagagcta ttctccatgt actggcaaga cctgatttct
2101 gagcatttaa tatggatgcc gtgggagtac aaaagtggag tgtggcctga gtaatgcatt
2161 atgggtggtt taccatttct tgaggtaaaa gcatcacatg aacttgtaaa ggaatttaaa
2221 aatcctactt tcataataag ttgcataggt ttaataattt ttaattatat ggcttgagtt
2281 taaattgtaa taggcgtaac taattttaac tctataatgt gttcattctg gaataatcct
2341 aaacatatga attatgtttg catgttcact tccaagagcc tttttttgaa aaaaagcttt
2401 ttttgaatca tcaagtcttt cacatttaaa taaagtgttt gaaagcttta tttaaaaaaa
2461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2521 aagaaaaaaa
//