LOCUS AK316492 1897 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA, FLJ79391 complete cds, highly similar to DNA
topoisomerase 2-beta (EC 5.99.1.3).
ACCESSION AK316492
VERSION AK316492.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1897)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1897
/clone="TKIDN2017670"
/clone_lib="TKIDN2"
/db_xref="H-InvDB:HIT000500105"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="kidney, tumor tissue"
CDS 255..1691
/codon_start=1
/note="highly similar to DNA topoisomerase 2-beta (EC
5.99.1.3)"
/protein_id="BAH14863.1"
/transl_table=1
/translation="MSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAF
VEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMK
ADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKK
EPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAE
RPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFS
PGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLK
QTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDPDSEFGIPKKTTTPKGK
GRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLP
RTGRARKEVKYFAESDEEEDDVDFAMFN"
BASE COUNT 659 a 326 c 406 g 506 t
ORIGIN
1 tctgacaaga tgcagtgata aaacattaat tctactctct taactttaaa atgtggtatc
61 ctctcatttt gttactgagt acctgaggaa atcagttaat ttttcatcag attcctggat
121 aaatagggat agtgtatacc attccttctg aagatcttat tgcagcagaa gaggatgaaa
181 cacaaaacca gcatgatgat agttcctccg attcaggaac tccttcaggc ccagatttta
241 attatatttt aaatatgtct ctgtggtctc ttactaaaga aaaagttgaa gaactgatta
301 aacagagaga tgcaaaaggg cgagaggtca atgatcttaa aagaaaatct ccttcagatc
361 tttggaaaga ggatttagcg gcatttgttg aagaactgga taaagtggaa tctcaagaac
421 gagaagatgt tctggctgga atgtctggaa aagcaattaa aggtaaagtt ggcaaaccta
481 aggtgaagaa actccagttg gaagagacaa tgccctcacc ttatggcaga agaataattc
541 ctgaaattac agctatgaag gcagatgcca gcaaaaagtt gctgaagaag aagaagggtg
601 atcttgatac tgcagcagta aaagtggaat ttgatgaaga attcagtgga gcaccagtag
661 aaggtgcagg agaagaggca ttgactccat cagttcctat aaataaaggt cccaaaccta
721 agagggagaa gaaggagcct ggtaccagag tgagaaaaac acctacatca tctggtaaac
781 ctagtgcaaa gaaagtgaag aaacggaatc cttggtcaga tgatgaatcc aagtcagaaa
841 gtgatttgga agaaacagaa cctgtggtta ttccaagaga ttctttgctt aggagagcag
901 cagccgaaag acctaaatac acatttgatt tctcagaaga agaggatgat gatgctgatg
961 atgatgatga tgacaataat gatttagagg aattgaaagt taaagcatct cccataacaa
1021 atgatgggga agatgaattt gttccttcag atgggttaga taaagatgaa tatacatttt
1081 caccaggcaa atcaaaagcc actccagaaa aatctttgca tgacaaaaaa agtcaggatt
1141 ttggaaatct cttctcattt ccttcatatt ctcagaagtc agaagatgat tcagctaaat
1201 ttgacagtaa tgaagaagat tctgcttctg ttttttcacc atcatttggt ctgaaacaga
1261 cagataaagt tccaagtaaa acggtagctg ctaaaaaggg aaaaccgtct tcagatacag
1321 tccctaagcc caagagagcc ccaaaacaga agaaagtagt agaggctgta aactctgacc
1381 cggattcaga atttggcatt ccaaagaaga ctacaacacc aaaaggtaaa ggccgagggg
1441 caaagaaaag gaaagcatct ggctctgaaa atgaaggcga ttataaccct ggcaggaaaa
1501 catccaaaac aacaagcaag aaaccgaaga agacatcttt tgatcaggat tcagatgtgg
1561 acatcttccc ctcagacttc cctactgagc caccttctct gccacgaacc ggtcgggcta
1621 ggaaagaagt aaaatatttt gcagagtctg atgaagaaga agatgatgtt gattttgcaa
1681 tgtttaatta agtgcccaaa gagcacaaac atttttcaac aaatatcttg tgttgtcctt
1741 ttgtcttctc tgtctcagac ttttgtacat ctggcttatt ttaatgtgat gatgtaattg
1801 acggtttttt attattgtgg taggcctttt aacattttgt tcttacacat acagttttat
1861 gctctttttt actcattgaa atgtcacgta ctgtctg
//