LOCUS AK316239 2202 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA, FLJ79138 complete cds, highly similar to
Epidermal growth factor receptor kinase substrate 8.
ACCESSION AK316239
VERSION AK316239.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2202)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2202
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3008152"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000499852"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 400..2088
/codon_start=1
/note="highly similar to Epidermal growth factor receptor
kinase substrate 8"
/protein_id="BAH14610.1"
/transl_table=1
/translation="MMAARIDRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKNK
KGKRKGPGEGVLTLRAKPPPPDEFLDCFQKFKHGFNLLAKLKSHIQNPSAADLVHFLF
TPLNMVVQATGGPELASSVLSPLLNKDTIDFLNYTVNGDERQLWMSLGGTWMKARAEW
PKEQFIPPYVPRFRNGWEPPMLNFMGATMEQDLYQLAESVANVAEHQRKQEIKRLSTE
HSSVSEYHPADGYAFSSNIYTRGSHLDQGEAAVAFKPTSNRHIDRNYEPLKTQPKKYA
KSKYDFVARNNSELSVLKDDILEILDDRKQWWKVRNASGDSGFVPNNILDIVRPPESG
LGRADPPYTHTIQKQRMEYGPRPADTPPAPSPPPTPAPVPVPLPPSTPAPVPVSKVPA
NITRQNSSSSDSGGSIVRDSQRHKQLPVDRRKSQMEEVQDELIHRLTIGRSAAQKKFH
VPRQNVPVINITYDSTPEDVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRTVCP
EGARVYSQITVQKAALEDSSGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH"
BASE COUNT 690 a 442 c 489 g 581 t
ORIGIN
1 aataccctga atggtcttaa gacatttatt ttttaatgtg aaaatttctg tctgctttta
61 aagcatgtag cttaaaatta atgagcccaa cattgtgggt tttaagctta attacactag
121 aattttaaga agcactctgc tgtcagaact ccgaaagctt tattgtagtc acagttggaa
181 tgtataaaaa ttatagctct gttgcattag aacttagttg accagctttt gagaagtaaa
241 agcttgactg ctggtttata tttctggctt gtgtaggaat taaagggtgc taagttaagg
301 ttggttctac aggtagctga ctcagggaag agagagaatg gggtgatatt acaagttgag
361 aaaccaaggc agtatcatga gcaggaagaa acacctgaga tgatggcagc ccgcattgac
421 agagatgtgc aaatcttaaa ccacattttg gatgacattg aattttttat cacaaaactc
481 caaaaagcag cagaagcatt ttctgagctt tctaaaagga agaaaaacaa gaaaggtaaa
541 aggaaaggac caggagaggg tgttttaacg ctgcgggcaa aacctccacc tcctgatgaa
601 tttcttgact gtttccaaaa gtttaaacac ggatttaacc ttctggccaa actgaagtct
661 catattcaga atcctagtgc tgcagatttg gttcactttt tgtttactcc attaaatatg
721 gtggtgcagg caacaggagg tcctgaacta gccagttcag tacttagtcc cctattgaat
781 aaggacacaa ttgatttctt aaattatact gtcaatggtg atgaacggca gctgtggatg
841 tcattgggag gaacttggat gaaagccaga gcagagtggc caaaagaaca gtttattcca
901 ccatatgttc cacgattccg caatggctgg gagcccccaa tgctgaactt tatgggagcc
961 acaatggaac aagatcttta tcaactggca gaatctgtgg caaatgtagc agaacatcag
1021 cgcaaacagg aaataaaaag attatccaca gagcattcca gtgtatcaga gtatcatcca
1081 gccgatggct atgcgttcag tagcaacatt tacacaagag gatcccacct ggaccaaggg
1141 gaagctgctg ttgcttttaa gccaacttct aatcgccata tagatagaaa ttatgaacca
1201 ctcaaaacac aacccaagaa atatgccaaa tccaagtatg actttgtagc aaggaacaac
1261 agtgagctct cagttctaaa ggatgatatt ttagagatac ttgatgatcg gaagcaatgg
1321 tggaaagttc gaaatgcaag tggagactct ggatttgtgc caaataacat tttggatatt
1381 gtgagacctc cagaatctgg attggggcgt gctgatccac cttatactca tactatacag
1441 aaacaaagga tggagtatgg cccaagacca gctgatactc cccctgctcc atcacctcct
1501 ccaacaccag ctcctgttcc tgttcccctt cccccttcca ctccagcacc tgttcctgtg
1561 tcaaaggtcc cagcaaatat aacacgtcaa aacagcagct ccagtgacag tggtggcagt
1621 atcgtgcgag acagccagag acacaaacaa cttccggtgg accgaaggaa atctcagatg
1681 gaggaagtgc aagatgaact catccacaga ctgaccattg gtcggagtgc cgctcagaag
1741 aaattccatg tgccacggca gaacgtgcca gttatcaata tcacttacga ctccacacca
1801 gaggatgtga agacgtggtt acagtcaaag ggattcaacc ctgtgactgt caatagtctt
1861 ggagtattaa atggtgcaca acttttctct ctcaataagg atgaactgag gacagtctgc
1921 cctgaagggg cgagagtcta tagccaaatc actgtacaaa aagctgcatt ggaggatagc
1981 agtggcagct ccgagttaca agaaattatg agaagacgac aggaaaaaat cagtgctgcc
2041 gctagtgatt caggagtgga atcttttgat gaaggaagca gtcactaatt tgtttgtttg
2101 tatttaaact ccattgtttt tggcattatt ccaacatgct ttgttttaag aagccttgaa
2161 gggaatgtca gattcatttt tcttgatgta atttatcacc at
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