LOCUS AK316231 1829 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA, FLJ79130 complete cds, highly similar to
Hephaestin precursor (EC 1.-.-.-).
ACCESSION AK316231
VERSION AK316231.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1829)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1829
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2009772"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000499844"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 140..1816
/codon_start=1
/note="highly similar to Hephaestin precursor (EC
1.-.-.-)"
/protein_id="BAH14602.1"
/transl_table=1
/translation="MLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAW
HLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAG
SHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQS
EKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEV
GDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSA
CVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSW
YLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLA
MGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTD
HVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEML
ASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ"
BASE COUNT 449 a 427 c 503 g 450 t
ORIGIN
1 gggcccgctg ctggtgtgca gggctggtgc cttgggtgca gatggcaagc agaaaggggt
61 ggataaagaa ttctttcttc tcttcactgt gttggatgag aacaagagct ggtacagcaa
121 tgccaatcaa gcagctgcta tgttggattt ccgactgctt tcagaggata ttgagggctt
181 ccaagactcc aatcggatgc atgccattaa tgggtttctg ttctctaacc tgcccaggct
241 ggacatgtgc aagggtgaca cagtggcctg gcacctgctc ggcctgggca cagagactga
301 tgtgcatgga gtcatgttcc agggcaacac tgtgcagctt cagggcatga ggaagggtgc
361 agctatgctc tttcctcata cctttgtcat ggccatcatg cagcctgaca accttgggac
421 atttgagatt tattgccagg caggcagcca tcgagaagca gggatgaggg caatctataa
481 tgtctcccag tgtcctggcc accaagccac ccctcgccaa cgctaccaag ctgcaagaat
541 ctactatatc atggcagaag aagtagagtg ggactattgc cctgaccgga gctgggaacg
601 ggaatggcac aaccagtctg agaaggacag ttatggttac attttcctga gcaacaagga
661 tgggctcctg ggttccagat acaagaaagc tgtattcagg gaatacactg atggtacatt
721 caggatccct cggccaagga ctggaccaga agaacacttg ggaatcttgg gtccacttat
781 caaaggtgaa gttggtgata tcctgactgt ggtattcaag aataatgcca gccgccccta
841 ctctgtgcat gctcatggag tgctagaatc tactactgtc tggccactgg ctgctgagcc
901 tggtgaggtg gtcacttatc agtggaacat cccagagagg tctggccctg ggcccaatga
961 ctctgcttgt gtttcctgga tctattattc tgcagtggat cccatcaagg acatgtatag
1021 tggcctggtg gggcccttgg ctatctgcca aaagggcatc ctggagcccc atggaggacg
1081 gagtgacatg gatcgggaat ttgcattgtt gttcttgatt tttgatgaaa ataagtcttg
1141 gtatttggag gaaaacgtgg caacccatgg gtcccaggat ccaggcagta ttaacctaca
1201 ggatgaaact ttcttggaga gcaataaaat gcatgcaatc aatgggaaac tctatgccaa
1261 ccttaggggt cttaccatgt accaaggaga acgagtggcc tggtacatgc tggccatggg
1321 ccaagatgtg gatctacaca ccatccactt tcatgcagag agcttcctct atcggaatgg
1381 cgagaactac cgggcagatg tggtggatct gttcccaggg acttttgagg ttgtggagat
1441 ggtggccagc aaccctggga catggctgat gcactgccat gtgactgacc atgtccatgc
1501 tggcatggag accctcttca ctgttttttc tcgaacagaa cacttaagcc ctctcaccgt
1561 catcaccaaa gagactgaaa aagcagtgcc ccccagagac attgaagaag gcaatgtgaa
1621 gatgctgggc atgcagatcc ccataaagaa tgttgagatg ctggcctctg ttttggttgc
1681 cattagtgtc acccttctgc tcgttgttct ggctcttggt ggagtggttt ggtaccaaca
1741 tcgacagaga aagctacgac gcaataggag gtccatcctg gatgacagct tcaagcttct
1801 gtctttcaaa cagtaacatc tggagcctg
//