LOCUS AK316151 2548 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA, FLJ79050 complete cds, highly similar to DNA
polymerase eta (EC 2.7.7.7).
ACCESSION AK316151
VERSION AK316151.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2548)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2548
/cell_type="normal dermal fibroblasts (Neonatal Skin)
(NHDF2564)"
/clone="DFNES2003046"
/clone_lib="DFNES2"
/db_xref="H-InvDB:HIT000499764"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal dermal fibroblasts"
/organism="Homo sapiens"
CDS 306..2261
/codon_start=1
/note="highly similar to DNA polymerase eta (EC 2.7.7.7)"
/protein_id="BAH14522.1"
/transl_table=1
/translation="MWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRL
AVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKE
GMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVL
AKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQ
FTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQ
WWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMS
HDAFTVIKNCNTSGIQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKV
PVTSSEAKTQGSGPAVTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQAPMSNS
PSKPSLPFQTSQSTGTEPFFKQKSLLLKQKQLNNSSVSSPQQNPWSNCKALPNSLPTE
YPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHASSASKSVLEVTQKATPNPSLLA
AEDQVPCEKCGSLVPVWDMPEHMDYHFALELQKSFLQPHSSNPQVVSAVSHQGKRNPK
SPLACTNKRPRPEGMQTLESFFKPLTH"
BASE COUNT 686 a 649 c 573 g 640 t
ORIGIN
1 agtctggcgg ctgcattgct gggcgcgccg ctctcgtctg atccctgctg gggacggttg
61 cccgggcagg atcctttacg atcccttctc ggtttctccg tcgtcacagg gaataaatct
121 cgctcgaaac tcactggacc gctcctagaa aggcgaaaag atattcagga gcccttccat
181 tttccttcca gtaggcaccg aacccagcat tttcggcaac cgctgctggc agttttgcca
241 ggtgtttgtt accttgaata attgcagtga gttatgaagc tcgtgcattt ggagtcacta
301 gaagtatgtg ggcagatgat gctaagaagt tatgtccaga tcttctactg gcacaagttc
361 gtgagtcccg tgggaaagct aacctcacca agtaccggga agccagtgtt gaagtgatgg
421 agataatgtc tcgtcttgct gtgattgaac gtgccagcat tgatgaggct tacgtagatc
481 tgaccagtgc tgtacaagag agactacaaa agctacaagg tcagcctatc tcggcagact
541 tgttgccaag cacttacatt gaagggttgc cccaaggccc tacaacggca gaagagactg
601 ttcagaaaga ggggatgcga aaacaaggct tatttcaatg gctcgattct cttcagattg
661 ataacctcac ctctccagac ctgcagctca ccgtgggagc agtgattgtg gaggaaatga
721 gagcagccat agagagggag actggttttc agtgttcagc tggaatttca cacaataagg
781 tcctggcaaa actggcctgt ggactaaaca agcccaaccg ccaaaccctg gtttcacatg
841 ggtcagtccc acagctcttc agccaaatgc ccattcgcaa aatccgtagt cttggaggaa
901 agctaggggc ctctgtcatt gagatcctag ggatagaata catgggtgaa ctgacccagt
961 tcactgaatc ccagctccag agtcattttg gggagaagaa tgggtcttgg ctatatgcca
1021 tgtgccgagg gattgaacat gatccagtta aacccaggca actacccaaa accattggct
1081 gtagtaagaa cttcccagga aaaacagctc ttgctactcg ggaacaggta caatggtggc
1141 tgttgcaatt agcccaggaa ctagaggaga gactgactaa agaccgaaat gataatgaca
1201 gggtagccac ccagctggtt gtgagcattc gtgtacaagg agacaaacgc ctcagcagcc
1261 tgcgccgctg ctgtgccctt acccgctatg atgctcacaa gatgagccat gatgcattta
1321 ctgtcatcaa gaactgtaat acttctggaa tccagacaga atggtctcct cctctcacaa
1381 tgcttttcct ctgtgctaca aaattttctg cctctgcccc ttcatcttct acagacatca
1441 ccagcttctt gagcagtgac ccaagttctc tgccaaaggt gccagttacc agctcagaag
1501 ctaagaccca gggaagtggc ccagcggtga cagccactaa gaaagcaacc acgtctctgg
1561 aatcattctt ccaaaaagct gcagaaaggc agaaagttaa agaagcttcg ctttcatctc
1621 ttactgctcc cactcaggct cccatgagca attcaccatc caagccctca ttaccttttc
1681 aaaccagtca aagtacagga actgagccct tctttaagca gaaaagtctg cttctaaagc
1741 agaaacagct taataattct tcagtttctt ccccccaaca aaacccatgg tccaactgta
1801 aagcattacc aaactcttta ccaacagagt atccagggtg tgtccctgtt tgtgaagggg
1861 tgtcgaagct agaagaatcc tctaaagcaa ctcctgcaga gatggatttg gcccacaaca
1921 gccaaagcat gcacgcctct tcagcttcca aatctgtgct ggaggtgact cagaaagcaa
1981 ccccaaatcc aagtcttcta gctgctgagg accaagtgcc ctgtgagaag tgtggctccc
2041 tggtaccggt atgggatatg ccagaacaca tggactatca ttttgcattg gagttgcaga
2101 aatccttttt gcagccccac tcttcaaacc cccaggttgt ttctgccgta tctcatcaag
2161 gcaaaagaaa tcccaagagc cctttggcct gcactaataa acgccccagg cctgagggca
2221 tgcaaacatt ggaatcattt tttaagccat taacacatta gtgctgccct caggcttgcc
2281 tgtaggattt aatatttttt atctttacag atctttatct ttaatatttt atctttacag
2341 atttccctga gaaagggaat tatgaaattt ttaatacaaa aaataatcca tttaggtgct
2401 gagttacggt cccatctctt cacaggctgg tcttgaactc ttgatctcaa gtgatccacc
2461 cgccctggcc tcccaaagtg ctgggattac aggcctgagc caccaggcca gccccaactt
2521 ctacttttta ttttatttat aaattggg
//