LOCUS AK315832 2229 bp mRNA linear HUM 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ79481 complete cds, highly similar to GMP
synthase (glutamine-hydrolyzing) (EC6.3.5.2).
ACCESSION AK315832
VERSION AK315832.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2229)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2229
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7020368"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000434698"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 111..2192
/codon_start=1
/note="highly similar to GMP synthase
(glutamine-hydrolyzing) (EC6.3.5.2)"
/protein_id="BAF98723.1"
/transl_table=1
/translation="MALCNGDSKLENAGGDLKDGHHHYEGAVVILDAGAQYGKVIDRR
VRELFVQSEIFPLETPAFAIKEQGFRAIIISGGPNSVYAEDAPWFDPAIFTIGKPVLG
ICYGMQMMNKVFGGTVHKKSVREDGVFNISVDNTCSLFRGLQKEEVVLLTHGDSVDKV
ADGFKVVARSGNIVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLYDIAGCSGTFT
VQNRELECIREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVIAVHIDNGFMR
KRESQSVEEALKKLGIQVKVINAAHSFYNGTTTLPISDEDRTPRKRISKTLNMTTSPE
EKRKIIGDTFVKIANEVIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKAELIKTHH
NDTELIRKLREEGKVIEPLKDFHKDEVRILGRELGLPEELVSRHPFPGPGLAIRVICA
EEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACTTEEDQEKLMQITSLHSLN
AFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNRVVYIFGP
PVKEPPTDVTPTFLTTGVLSTLRQADFEAHNILRESGYAGKISQMPVILTPLHFDRDP
LQKQPSCQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGISRIMYDL
TSKPPGTTEWE"
BASE COUNT 666 a 448 c 517 g 598 t
ORIGIN
1 tccattcggc gcttttctgg cggctggctc ctctccgctg ccggctgctc ctcgaccagg
61 cctccttctc aacctcagcc cgcggcgccg acccttccgg caccctcccg atggctctgt
121 gcaacggaga ctccaagctg gagaatgctg gaggagacct taaggatggc caccaccact
181 atgaaggagc tgttgtcatt ctggatgctg gtgctcagta cgggaaagtc atagaccgaa
241 gagtgaggga actgttcgtg cagtctgaaa ttttcccctt ggaaacacca gcatttgcta
301 taaaggaaca aggattccgt gctattatca tctctggagg acctaattct gtgtatgctg
361 aagatgctcc ctggtttgat ccagcaatat tcactattgg caagcctgtt cttggaattt
421 gctatggtat gcagatgatg aataaggtat ttggaggtac tgtgcacaaa aaaagtgtca
481 gagaagatgg agttttcaac attagtgtgg ataatacatg ttcattattc aggggccttc
541 agaaggaaga agttgttttg cttacacatg gagatagtgt agacaaagta gctgatggat
601 tcaaggttgt ggcacgttct ggaaacatag tagcaggcat agcaaatgaa tctaaaaagt
661 tatatggagc acagttccac cctgaagttg gccttacaga aaatggaaaa gtaatactga
721 agaatttcct ttatgatata gctggatgca gtggaacctt caccgtgcag aacagagaac
781 ttgagtgtat tcgagagatc aaagagagag taggcacgtc aaaagttttg gttttactca
841 gtggtggagt agactcaaca gtttgtacag ctttgctaaa tcgtgctttg aaccaagaac
901 aagtcattgc tgtgcacatt gataatggct ttatgagaaa acgagaaagc cagtctgttg
961 aagaggccct caaaaagctt ggaattcagg tcaaagtgat aaatgctgct cattctttct
1021 acaatggaac aacaacccta ccaatatcag atgaagatag aaccccacgg aaaagaatta
1081 gcaaaacgtt aaatatgacc acaagtcctg aagagaaaag aaaaatcatt ggggatactt
1141 ttgttaagat tgccaatgaa gtaattggag aaatgaactt gaaaccagag gaggttttcc
1201 ttgcccaagg tactttacgg cctgatctaa ttgaaagtgc atcccttgtt gcaagtggca
1261 aagctgaact catcaaaacc catcacaatg acacagagct catcagaaag ttgagagagg
1321 agggaaaagt aatagaacct ctgaaagatt ttcataaaga tgaagtgaga attttgggca
1381 gagaacttgg acttccagaa gagttagttt ccaggcatcc atttccaggt cctggcctgg
1441 caatcagagt aatatgtgct gaagaacctt atatttgtaa ggactttcct gaaaccaaca
1501 atattttgaa aatagtagct gatttttctg caagtgttaa aaagccacat accctattac
1561 agagagtcaa agcctgcaca acagaagagg atcaggagaa gctgatgcaa attaccagtc
1621 tgcattcact gaatgccttc ttgctgccaa ttaaaactgt aggtgtgcag ggtgactgtc
1681 gttcctacag ttacgtgtgt ggaatctcca gtaaagatga acctgactgg gaatcactta
1741 tttttctggc taggcttata cctcgcatgt gtcacaacgt taacagagtt gtttatatat
1801 ttggcccacc agttaaagaa cctcctacag atgttactcc cactttcttg acaacagggg
1861 tgctcagtac tttacgccaa gctgattttg aggcccataa cattctcagg gagtctgggt
1921 atgctgggaa aatcagccag atgccggtga ttttgacacc attacatttt gatcgggacc
1981 cacttcaaaa gcagccttca tgccagagat ctgtggttat tcgaaccttt attactagtg
2041 acttcatgac tggtatacct gcaacacctg gcaatgagat ccctgtagag gtggtattaa
2101 agatggtcac tgagattaag aagattcctg gtatttctcg aattatgtat gacttaacat
2161 caaagccccc aggaactact gagtgggagt aataaacttc ttgttctatt aaagtaccgt
2221 gtgcagttt
//