LOCUS AK315825 1722 bp mRNA linear HUM 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ79474 complete cds, highly similar to
Prostaglandin E2 receptor, EP3 subtype.
ACCESSION AK315825
VERSION AK315825.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1722)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1722
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2015950"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000434691"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 160..1332
/codon_start=1
/note="highly similar to Prostaglandin E2 receptor, EP3
subtype"
/protein_id="BAF98716.1"
/transl_table=1
/translation="MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSG
EDCGSVSVAFPITMLLTGFVGNALAMLLVSRSYRRRESKRKKSFLLCIGWLALTDLVG
QLLTTPVVIVVYLSKQRWEHIDPSGRLCTFFGLTMTVFGLSSLFIASAMAVERALAIR
APHWYASHMKTRATRAVLLGVWLAVLAFALLPVLGVGQYTVQWPGTWCFISTGRGGNG
TSSSHNWGNLFFASAFAFLGLLALTVTFSCNLATIKALVSRCRAKATASQSSAQWGRI
TTETAIQLMGIMCVLSVCWSPLLIMMLKMIFNQTSVEHCKTHTEKQKECNFFLIAVRL
ASLNQILDPWVYLLLRKILLRKFCQIRYHTNNYASSSTSLPCQCSSTLMWSDHLER"
BASE COUNT 337 a 505 c 456 g 424 t
ORIGIN
1 gtcccagcag cggagtaggg cggcggctgc gccccgcacc atggggggca gcccagcccc
61 agccgcggta aacgccgacc tccgccgccg cccgcgccgc gtctgccccc tcccgctgcg
121 gctctctgga cgccatcccc tcctcacctc gaagccaaca tgaaggagac ccggggctac
181 ggaggggatg cccccttctg cacccgcctc aaccactcct acacaggcat gtgggcgccc
241 gagcgttccg ccgaggcgcg gggcaacctc acgcgccctc cagggtctgg cgaggattgc
301 ggatcggtgt ccgtggcctt cccgatcacc atgctgctca ctggtttcgt gggcaacgca
361 ctggccatgc tgctcgtgtc gcgcagctac cggcgccggg agagcaagcg caagaagtcc
421 ttcctgctgt gcatcggctg gctggcgctc accgacctgg tcgggcagct tctcaccacc
481 ccggtcgtca tcgtcgtgta cctgtccaag cagcgttggg agcacatcga cccgtcgggg
541 cggctctgca cctttttcgg gctgaccatg actgttttcg ggctctcctc gttgttcatc
601 gccagcgcca tggccgtcga gcgggcgctg gccatcaggg cgccgcactg gtatgcgagc
661 cacatgaaga cgcgtgccac ccgcgctgtg ctgctcggcg tgtggctggc cgtgctcgcc
721 ttcgccctgc tgccggtgct gggcgtgggc cagtacaccg tccagtggcc cgggacgtgg
781 tgcttcatca gcaccgggcg agggggcaac gggactagct cttcgcataa ctggggcaac
841 cttttcttcg cctctgcctt tgccttcctg gggctcttgg cgctgacagt caccttttcc
901 tgcaacctgg ccaccattaa ggccctggtg tcccgctgcc gggccaaggc cacggcatct
961 cagtccagtg cccagtgggg ccgcatcacg accgagacgg ccattcagct tatggggatc
1021 atgtgcgtgc tgtcggtctg ctggtctccg ctcctgataa tgatgttgaa aatgatcttc
1081 aatcagacat cagttgagca ctgcaagaca cacacggaga agcagaaaga atgcaacttc
1141 ttcttaatag ctgttcgcct ggcttcactg aaccagatct tggatccttg ggtttacctg
1201 ctgttaagaa agatccttct tcgaaagttt tgccagatca ggtaccacac aaacaactat
1261 gcatccagct ccacctcctt accctgccag tgttcctcaa ccttgatgtg gagcgaccat
1321 ttggaaagat aatgaaagaa cggagttgga cattttattg caattcctgc ttccctgaat
1381 ttgcatattt cttcccacct gagaaggata attatatatt ttaatttgga ttatttcttc
1441 atttttatct ttttatttta atgattgttt tgtcagtaat acccatggag atcaacttta
1501 ttattataat ccatgcctct gaatattaga ttggtttctt ggatgggatt ttgaatatgc
1561 atttaagaag ttgggaagaa tttcacagat gatgattgga ggaaaagtga tgaaaagaaa
1621 gacctgtgtt ccaggagttt tctccaactt caaaccttta cgtgaatctt aaccaaagtg
1681 gacatcttta catttcatga tagcttgctt ttgcaatatg ag
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