LOCUS AK315461 998 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ96520, Homo sapiens secreted phosphoprotein
1 (osteopontin, bonesialoprotein I, early T-lymphocyte activation
1) (SPP1), mRNA.
ACCESSION AK315461
VERSION AK315461.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 998)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..998
/clone="KIDNE2013695"
/clone_lib="KIDNE2"
/db_xref="H-InvDB:HIT000434327"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS 96..998
/note="Homo sapiens secreted phosphoprotein 1
(osteopontin, bonesialoprotein I, early T-lymphocyte
activation 1) (SPP1), mRNA"
/protein_id="BAG37848.1"
/translation="MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWL
NPDPSQKQNLLAPQTLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDD
SHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKF
RRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDD
QSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPK
SKEEDKHLKFRISHELDSASSEVN"
BASE COUNT 300 a 244 c 234 g 220 t
ORIGIN
1 aggaggaggc agagcacagc atcgtcggga ccagactcgt ctcaggccag ttgcagcctt
61 ctcagccaaa cgccgaccaa ggaaaactca ctaccatgag aattgcagtg atttgctttt
121 gcctcctagg catcacctgt gccataccag ttaaacaggc tgattctgga agttctgagg
181 aaaagcagct ttacaacaaa tacccagatg ctgtggccac atggctaaac cctgacccat
241 ctcagaagca gaatctccta gccccacaga cccttccaag taagtccaac gaaagccatg
301 accacatgga tgatatggat gatgaagatg atgatgacca tgtggacagc caggactcca
361 ttgactcgaa cgactctgat gatgtagatg acactgatga ttctcaccag tctgatgagt
421 ctcaccattc tgatgaatct gatgaactgg tcactgattt tcccacggac ctgccagcaa
481 ccgaagtttt cactccagtt gtccccacag tagacacata tgatggccga ggtgatagtg
541 tggtttatgg actgaggtca aaatctaaga agtttcgcag acctgacatc cagtaccctg
601 atgctacaga cgaggacatc acctcacaca tggaaagcga ggagttgaat ggtgcataca
661 aggccatccc cgttgcccag gacctgaacg cgccttctga ttgggacagc cgtgggaagg
721 acagttatga aacgagtcag ctggatgacc agagtgctga aacccacagc cacaagcagt
781 ccagattata taagcggaaa gccaatgatg agagcaatga gcattccgat gtgattgata
841 gtcaggaact ttccaaagtc agccgtgaat tccacagcca tgaatttcac agccatgaag
901 atatgctggt tgtagacccc aaaagtaagg aagaagataa acacctgaaa tttcgtattt
961 ctcatgaatt agatagtgca tcttctgagg tcaattaa
//