LOCUS AK315417 1447 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ96469, Homo sapiens enolase 1, (alpha)
(ENO1), mRNA.
ACCESSION AK315417
VERSION AK315417.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1447)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1447
/clone="D9OST1000026"
/clone_lib="D9OST1"
/db_xref="H-InvDB:HIT000434283"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 9-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 143..1447
/note="Homo sapiens enolase 1, (alpha) (ENO1), mRNA"
/protein_id="BAG37806.1"
/translation="MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIY
EALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTEN
KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAG
NKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNIL
ENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQL
ADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNE
KSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQ
IKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK"
BASE COUNT 348 a 371 c 415 g 313 t
ORIGIN
1 aggaagtcgg cgcgggcggc gcggacagta tctgtgggta cccggagcac ggagatctcg
61 ccggctttac gttcacctcg gtgtctgcag caccctccgc ttcctctcct aggcgacgag
121 acccagtggc tagaagttca ccatgtctat tctcaagatc catgccaggg agatctttga
181 ctctcgcggg aatcccactg ttgaggttga tctcttcacc tcaaaaggtc tcttcagagc
241 tgctgtgccc agtggtgctt caactggtat ctatgaggcc ctagagctcc gggacaatga
301 taagactcgc tatatgggga agggtgtctc aaaggctgtt gagcacatca ataaaactat
361 tgcgcctgcc ctggttagca agaaactgaa cgtcacagaa caagagaaga ttgacaaact
421 gatgatcgag atggatggaa cagaaaataa atctaagttt ggtgcgaacg ccattctggg
481 ggtgtccctt gccgtctgca aagctggtgc cgttgagaag ggggtccccc tgtaccgcca
541 catcgctgac ttggctggca actctgaagt catcctgcca gtcccggcgt tcaatgtcat
601 caatggcggt tctcatgctg gcaacaagct ggccatgcag gagttcatga tcctcccagt
661 cggtgcagca aacttcaggg aagccatgcg cattggagca gaggtttacc acaacctgaa
721 gaatgtcatc aaggagaaat atgggaaaga tgccaccaat gtgggggatg aaggcgggtt
781 tgctcccaac atcctggaga ataaagaagg cctggagctg ctgaagactg ctattgggaa
841 agctggctac actgataagg tggtcatcgg catggacgta gcggcctccg agttcttcag
901 gtctgggaag tatgacctgg acttcaagtc tcccgatgac cccagcaggt acatctcgcc
961 tgaccagctg gctgacctgt acaagtcctt catcaaggac tacccagtgg tgtctatcga
1021 agatcccttt gaccaggatg actggggagc ttggcagaag ttcacagcca gtgcaggaat
1081 ccaggtagtg ggggatgatc tcacagtgac caacccaaag aggatcgcca aggccgtgaa
1141 cgagaagtcc tgcaactgcc tcctgctcaa agtcaaccag attggctccg tgaccgagtc
1201 tcttcaggcg tgcaagctgg cccaggccaa tggttggggc gtcatggtgt ctcatcgttc
1261 gggggagact gaagatacct tcatcgctga cctggttgtg gggctgtgca ctgggcagat
1321 caagactggt gccccttgcc gatctgagcg cttggccaag tacaaccagc tcctcagaat
1381 tgaagaggag ctgggcagca aggctaagtt tgccggcagg aacttcagaa accccttggc
1441 caagtaa
//