LOCUS AK315360 1750 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ96405, Homo sapiens phosphoglycerate
dehydrogenase (PHGDH), mRNA.
ACCESSION AK315360
VERSION AK315360.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1750)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1750
/cell_line="GI1"
/cell_type="glioma separated from gliosarcoma"
/clone="BGGI12000728"
/clone_lib="BGGI12"
/db_xref="H-InvDB:HIT000434226"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 149..1750
/note="Homo sapiens phosphoglycerate dehydrogenase
(PHGDH), mRNA"
/protein_id="BAG37755.1"
/translation="MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAE
LQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGN
SLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREV
ATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLN
DNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHE
NVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIG
LAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVN
AKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG
AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHV
MGISSLLPSLEAWKQHVTEAFQFHF"
BASE COUNT 372 a 478 c 550 g 350 t
ORIGIN
1 agactgtagg aggaggagga ggaggagatg actggggagc gggagctcga gaatactgcc
61 cagttactct agcgcgccag gccgaaccgc agcttcttgg cttaggtact tctactcaca
121 gcggccgatt ccgaggccaa ctccagcaat ggcttttgca aatctgcgga aagtgctcat
181 cagtgacagc ctggaccctt gctgccggaa gatcttgcaa gatggagggc tgcaggtggt
241 ggaaaagcag aaccttagca aagaggagct gatagcggag ctgcaggact gtgaaggcct
301 tattgttcgc tctgccacca aggtgaccgc tgatgtcatc aacgcagctg agaaactcca
361 ggtggtgggc agggctggca caggtgtgga caatgtggat ctggaggccg caacaaggaa
421 gggcatcttg gttatgaaca cccccaatgg gaacagcctc agtgccgcag aactcacttg
481 tggaatgatc atgtgcctgg ccaggcagat tccccaggcg acggcttcga tgaaggacgg
541 caaatgggag cggaagaagt tcatgggaac agagctgaat ggaaagaccc tgggaattct
601 tggcctgggc aggattggga gagaggtagc tacccggatg cagtcctttg ggatgaagac
661 tatagggtat gaccccatca tttccccaga ggtctcggcc tcctttggtg ttcagcagct
721 gcccctggag gagatctggc ctctctgtga tttcatcact gtgcacactc ctctcctgcc
781 ctccacgaca ggcttgctga atgacaacac ctttgcccag tgcaagaagg gggtgcgtgt
841 ggtgaactgt gcccgtggag ggatcgtgga cgaaggcgcc ctgctccggg ccctgcagtc
901 tggccagtgt gccggggctg cactggacgt gtttacggaa gagccgccac gggaccgggc
961 cttggtggac catgagaatg tcatcagctg tccccacctg ggtgccagca ccaaggaggc
1021 tcagagccgc tgtggggagg aaattgctgt tcagttcgtg gacatggtga aggggaaatc
1081 tctcacgggg gttgtgaatg cccaggccct taccagtgcc ttctctccac acaccaagcc
1141 ttggattggt ctggcagaag ctctggggac actgatgcga gcctgggctg ggtcccccaa
1201 agggaccatc caggtgataa cacagggaac atccctgaag aatgctggga actgcctaag
1261 ccccgcagtc attgtcggcc tcctgaaaga ggcttccaag caggcggatg tgaacttggt
1321 gaacgctaag ctgctggtga aagaggctgg cctcaatgtc accacctccc acagccctgc
1381 tgcaccaggg gagcaaggct tcggggaatg cctcctggcc gtggccctgg caggcgcccc
1441 ttaccaggct gtgggcttgg tccaaggcac tacgcctgta ctgcaggggc tcaatggagc
1501 tgtcttcagg ccagaagtgc ctctccgcag ggacctgccc ctgctcctat tccggactca
1561 gacctctgac cctgcaatgc tgcctaccat gattggcctc ctggcagagg caggcgtgcg
1621 gctgctgtcc taccagactt cactggtgtc agatggggag acctggcacg tcatgggcat
1681 ctcctccttg ctgcccagcc tggaagcgtg gaagcagcat gtgactgaag ccttccagtt
1741 ccacttctaa
//