LOCUS AK315177 2139 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ96154, highly similar to Homo sapiens heat
shock 70kDa protein 9B (mortalin-2) (HSPA9B),nuclear gene encoding
mitochondrial protein, mRNA.
ACCESSION AK315177
VERSION AK315177.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2139)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2139
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8006251"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000434043"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 100..2139
/note="highly similar to Homo sapiens heat shock 70kDa
protein 9B (mortalin-2) (HSPA9B),nuclear gene encoding
mitochondrial protein, mRNA"
/protein_id="BAG37618.1"
/translation="MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRR
DYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVG
MPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKL
YSPSQIGAFVLMKMKVTAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLR
VINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGE
DFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTM
DSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRM
PKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETL
GGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGI
PPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAE
KYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLAR
KDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ"
BASE COUNT 620 a 442 c 565 g 512 t
ORIGIN
1 gtggttggag gtttccagaa gcgctgccgc caccgcatcg cgcagctctt tgccgtcgga
61 gcgcttgttt gctgcctcgt actcctccat ttatccgcca tgataagtgc cagccgagct
121 gcagcagccc gtctcgtggg cgccgcagcc tcccggggcc ctacggccgc ccgccaccag
181 gatagctgga atggccttag tcatgaggct tttagacttg tttcaaggcg ggattatgca
241 tcagaagcaa tcaagggagc agttgttggt attgatttgg gtactaccaa ctcctgcgtg
301 gcagttatgg aaggtaaaca agcaaaggtg ctggagaatg ccgaaggtgc cagaaccacc
361 ccttcagttg tggcctttac agcagatggt gagcgacttg ttggaatgcc ggccaagcga
421 caggctgtca ccaacccaaa caatacattt tatgctacca agcgtctcat tggccggcga
481 tatgatgatc ctgaagtaca gaaagacatt aaaaatgttc cctttaaaat tgtccgtgcc
541 tccaatggtg atgcctgggt tgaggctcat gggaaattgt attctccgag tcagattgga
601 gcatttgtgt tgatgaagat gaaagtgact gcagaaaatt acttggggca cacagcaaaa
661 aatgctgtga tcacagtccc agcttatttc aatgactcgc agagacaggc cactaaagat
721 gctggccaga tatctggact gaatgtgctt cgggtgatta atgagcccac agctgctgct
781 cttgcctatg gtctagacaa atcagaagac aaagtcattg ctgtatatga tttaggtggt
841 ggaacttttg atatctctat cctggaaatt cagaaaggag tatttgaggt gaaatccaca
901 aatggggata ccttcttagg tggggaagac tttgaccagg ccttgctacg gcacattgtg
961 aaggagttca agagagagac aggggttgat ttgactaaag acaacatggc acttcagagg
1021 gtacgggaag ctgctgaaaa ggctaaatgt gaactctcct catctgtgca gactgacatc
1081 aatttgccct atcttacaat ggattcttct ggacccaagc atttgaatat gaagttgacc
1141 cgtgctcaat ttgaagggat tgtcactgat ctaatcagaa ggactatcgc tccatgccaa
1201 aaagctatgc aagatgcaga agtcagcaag agtgacatag gagaagtgat tcttgtgggt
1261 ggcatgacta ggatgcccaa ggttcagcag actgtacagg atctttttgg cagagcccca
1321 agtaaagctg tcaatcctga tgaggctgtg gccattggag ctgccattca gggaggtgtg
1381 ttggccggcg atgtcacgga tgtgctgctc cttgatgtca ctcccctgtc tctgggtatt
1441 gaaactctag gaggtgtctt taccaaactt attaatagga ataccactat tccaaccaag
1501 aagagccagg tattctctac tgccgctgat ggtcaaacgc aagtggaaat taaagtgtgt
1561 cagggtgaaa gagagatggc tggagacaac aaactccttg gacagtttac tttgattgga
1621 attccaccag cccctcgtgg agttcctcag attgaagtta catttgacat tgatgccaat
1681 gggatagtac atgtttctgc taaagataaa ggcacaggac gtgagcagca gattgtaatc
1741 cagtcttctg gtggattaag caaagatgat attgaaaata tggttaaaaa tgcagagaaa
1801 tatgctgaag aagaccggcg aaagaaggaa cgagttgaag cagttaatat ggctgaagga
1861 atcattcacg acacagaaac caagatggaa gaattcaagg accaattacc tgctgatgag
1921 tgcaacaagc tgaaagaaga gatttccaaa atgagggagc tcctggctag aaaagacagc
1981 gaaacaggag aaaatattag acaggcagca tcctctcttc agcaggcatc actgaagctg
2041 ttcgaaatgg catacaaaaa gatggcatct gagcgagaag gctctggaag ttctggcact
2101 ggggaacaaa aggaagatca aaaggaggaa aaacagtaa
//