LOCUS AK315146 2171 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ96117, highly similar to Homo sapiens
calpastatin (CAST), transcript variant 4, mRNA.
ACCESSION AK315146
VERSION AK315146.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2171)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2171
/clone="TRACH3012712"
/clone_lib="TRACH3"
/db_xref="H-InvDB:HIT000434012"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="trachea"
CDS 399..2171
/note="highly similar to Homo sapiens calpastatin (CAST),
transcript variant 4, mRNA"
/protein_id="BAG37594.1"
/translation="MISAGGESVAGITAISGKPGDKKKGKKSLTPAVPVESKPDKPSG
KSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRE
LLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKK
RKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIP
SEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEE
SKAAAPAPVSEAVSRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGK
PVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDF
LLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLD
DALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNG
QDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSK
APKNGGKAKDSAKTTEETSKPKDD"
BASE COUNT 767 a 491 c 520 g 393 t
ORIGIN
1 agagagctgg gctggagctc caagcggaaa ccgcgggagc cgagcccagc taggaatgca
61 gacctcctga aaaccaagcc gaggactgcg gggtccggtg tccacgcaga gtgtcagctt
121 cctctggtgc aaccagcaag tcttccagta tgaatcccac agaaaccaag gctgtaaaaa
181 cagaacctga gaagaagtca cagtcaacca agctgtctgt ggttcatgag aaaaaatccc
241 aagaaggaaa gccataagaa cacacagagc caaaaagcct acccaagcag gcatcagata
301 caggaagtaa cgatgctcac aataaaaaag cagtttccag atcagctgaa cagcagccat
361 cagagaaatc aacagaacca aagactaaac cacaagacat gatttctgct ggtggagaga
421 gtgttgctgg tatcactgca atatctggca agccgggtga caagaaaaaa ggaaagaaat
481 cattaacccc agctgtgcca gttgaatcta aaccggataa accatcggga aagtcaggca
541 tggatgctgc tttggatgac ttaatagata ctttaggagg acctgaagaa actgaagaag
601 aaaatacaac gtatactgga ccagaagttt cagatccaat gagttccacc tacatagagg
661 aattgggtaa aagagaagtc acaattcctc caaaatatag ggaactattg gctaaaccca
721 tagggccaga tgatgctata gacgccttgt catctgactt cacctgtggg tcgcctacag
781 ctgctggaaa gaaaactgaa aaagaggaat ctacagaagt tttaaaagct cagtcagcag
841 ggacagtcag aagtgctgct ccaccccaag agaagaaaag aaaggtggag aaggatacaa
901 tgagtgatca agcactcgag gctctgtcgg cttcactggg cacccggcaa gcagaacctg
961 agctcgacct ccgctcaatt aaggaagtcg atgaggcaaa agctaaagaa gaaaaactag
1021 agaagtgtgg tgaggatgat gaaacaatcc catctgagta cagattaaaa ccagccacgg
1081 ataaagatgg aaaaccacta ttgccagagc ctgaagaaaa acccaagcct cggagtgaat
1141 cagaactcat tgatgaactt tcagaagatt ttgaccggtc tgaatgtaaa gagaaaccat
1201 ctaagccaac tgaaaagaca gaagaatcta aggccgctgc tccagctcct gtgtcggagg
1261 ctgtgtctcg gacctccatg tgtagtatac agtcagcacc ccctgagccg gctaccttga
1321 agggcacagt gccagatgat gctgtagaag ccttggctga tagcctgggg aaaaaggaag
1381 cagatccaga agatggaaaa cctgtgatgg ataaagtcaa ggagaaggcc aaagaagaag
1441 accgtgaaaa gcttggtgaa aaagaagaaa caattcctcc tgattataga ttagaagagg
1501 tcaaggataa agatggaaag ccactcctgc caaaagagtc taaggaacag cttccaccca
1561 tgagtgaaga cttccttctg gatgctttgt ctgaggactt ctctggtcca caaaatgctt
1621 catctcttaa atttgaagat gctaaacttg ctgctgccat ctctgaagtg gtttcccaaa
1681 ccccagcttc aacgacccaa gctggagccc caccccgtga tacctcgcag agtgacaaag
1741 acctcgatga tgccttggat aaactctctg acagtctagg acaaaggcag cctgacccag
1801 atgagaacaa accaatggaa gataaagtaa aggaaaaagc taaagctgaa catagagaca
1861 agcttggaga aagagatgac actatcccac ctgaatacag acatctcctg gatgataatg
1921 gacaggacaa accagtgaag ccacctacaa agaaatcaga ggattcaaag aaacctgcag
1981 atgaccaaga ccccattgat gctctctcag gagatctgga cagctgtccc tccactacag
2041 aaacctcaca gaacacagca aaggataagt gcaagaaggc tgcttccagc tccaaagcac
2101 ctaagaatgg aggtaaagcg aaggattcag caaagacaac agaggaaact tccaagccaa
2161 aagatgacta a
//