LOCUS AK314454 1728 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ95255, Homo sapiens nucleoporin 50kDa
(NUP50), transcript variant 2, mRNA.
ACCESSION AK314454
VERSION AK314454.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1728)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1728
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8005934"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000433320"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 322..1728
/note="Homo sapiens nucleoporin 50kDa (NUP50), transcript
variant 2, mRNA"
/protein_id="BAG37062.1"
/translation="MAKRNAEKELTDRNWDQEDEAEEVGTFSMASEEVLKNRAIKKAK
RRNVGFESDTGGAFKGFKGLVVPSGGGRFSGFGSGAGGKPLEGLSNGNNITSAPPFAS
AKAAADPKVAFGSLAANGPTTLVDKVSNPKTNGDSQQPSSSGLASSKACVGNAYHKQL
AALNCSVRDWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAE
TQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKK
VDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECK
GGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQ
LLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVK
TSEDADELHKILLEKKDA"
BASE COUNT 498 a 408 c 441 g 381 t
ORIGIN
1 gttttggggg tagattcgag ttacaaaatg gccgcccgga gtgtgttcgg cgcggttccc
61 ccagctgtct ctggctgaac cggcgctctc gcctccctgc cgaacacagc gtgaggagcc
121 cccccaggga catggtgttt gagtctctgg gcttgccgag cactaagtcc tctgagttcc
181 gcagcgcagc accggaagcg gccgagcgcg ctcagcccgg cgacccctgc gggctccaga
241 cccctgcgcc gctgcgcccc gggtttcgcc gcaaccaaga cccagcgagt gcagcggcgg
301 ccgccgagga ggttcgaaaa catggccaaa agaaatgccg agaaggaact gacagatagg
361 aattgggatc aagaagatga agctgaagag gtgggaacat tctccatggc cagtgaggaa
421 gtcttgaaga atagagccat aaagaaagca aagcgcagaa atgttggatt tgaatctgac
481 actggaggag cctttaaagg ttttaaaggt ttggtggtac cttctggagg aggacgcttt
541 tctggatttg gtagtggcgc tggagggaag cctttggaag gactgtcgaa tggaaacaac
601 ataaccagtg cccctccctt cgccagtgca aaggcagcgg cagatcccaa ggtagccttt
661 ggttctcttg ctgcaaatgg ccctaccacc ttggttgata aagtttcaaa tcccaaaact
721 aatggggaca gtcagcagcc ctcctcctct ggccttgctt ccagtaaagc ttgtgtcgga
781 aatgcctatc acaagcagtt ggccgccttg aactgctccg tgcgggattg gatagtgaag
841 cacgtgaata caaaccccct ctgtgatctg acacctatct ttaaagacta tgagaaatat
901 ttagcaaaca ttgaacagca acacgggaac agtggcagga attctgaaag tgaatctaac
961 aaagtggcag ctgaaacaca gtctccttcc ctttttggct caacaaaatt acagcaagag
1021 tcaacgtttt tgtttcatgg caacaaaact gaagatacac ctgacaagaa gatggaggtg
1081 gcatctgaaa agaaaacgga cccatcatca ctaggagcga caagtgcctc atttaatttc
1141 ggcaagaaag ttgatagctc tgttttgggc tcattaagct ctgtccccct gactggattt
1201 tctttctccc ctggaaactc cagtttattt ggcaaagata ctacccagag taaaccagtc
1261 tcttcaccat ttcccactaa accattggag ggccaagcag aaggtgacag tggtgaatgc
1321 aaaggtggag atgaagaaga gaatgatgag ccacccaaag tagtagttac cgaagtaaaa
1381 gaagaagatg ctttttactc caaaaagtgt aaactgtttt acaagaaaga caatgagttt
1441 aaagagaaag gcataggtac tctgcattta aaacctacag caaatcagaa gacacagctt
1501 ttggtgcggg cagacaccaa tttaggcaac atattgctga acgttctgat tccacccaat
1561 atgccatgta cgcgaacagg gaagaataac gttcttatcg tctgtgttcc aaatccacca
1621 attgacgaga agaatgccac catgccagtc accatgttga ttcgggtaaa aaccagcgag
1681 gatgcagacg agttgcacaa aattttactg gagaaaaagg atgcctga
//