LOCUS AK314089 1703 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ94765, highly similar to Homo sapiens
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1),
mRNA.
ACCESSION AK314089
VERSION AK314089.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1703)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
The reason of remaining 5'-end "N": This sequence was generated
from 5'-end region of cDNA insert including about 20 base of the
sequence before "N". This cDNA was obtained from oligo-capped cDNA
library by which method 5'-end of mRNA was efficiently cloned.
Therefore remaining 5'-end "N" is important for a detection of
transcription start site of this mRNA.
FEATURES Location/Qualifiers
source 1..1703
/clone="TLIVE2007887"
/clone_lib="TLIVE2"
/db_xref="H-InvDB:HIT000432955"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="liver, tumor tissue"
CDS 288..1703
/note="highly similar to Homo sapiens
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
(PFKFB1), mRNA"
/protein_id="BAG36785.1"
/translation="MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTM
VIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEAL
QIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESI
CNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYI
KIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGL
SVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVC
EEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVIC
HQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREK
PENVDITREPEEALDTVPAHY"
BASE COUNT 467 a 448 c 434 g 353 t
ORIGIN
1 ncacagccct tggagttcag aggcctctcc tgacctctgc ccaacctctc gccctctgca
61 cccaataccc cactagtaca tacaggagca gaggcaggct aggaccagag gaaagaagag
121 aggggactga agacagactt tgagagggct cagaaaccca gtacaccccc tgtcccagcc
181 ctgtccacct gctgctgtgg ccacagcgag agaaaagaca gctagtaaga taggaagtga
241 ggccaggtac cttgtgggca gtgatgtcat tcggtgcgac tcctaagatg tctccagaga
301 tgggagagct cacccaaacc aggttgcaga agatctggat tccacacagc agcggcagca
361 gcaggctgca acggagaagg ggctcatcca taccccagtt taccaattcc cccacaatgg
421 tgatcatggt gggtttacca gctcgaggca agacctatat ctccacaaag ctcacacgat
481 atctcaactg gataggaaca ccaactaaag tgtttaattt aggccagtat cgacgagagg
541 cagtgagcta caagaactat gaattctttc ttccagacaa catggaagcc ctgcaaatca
601 ggaagcagtg cgccctggca gccctgaagg atgttcacaa ctatctcagc catgaggaag
661 gtcatgttgc ggtttttgat gccaccaaca ctaccagaga acgacggtca ctgatcctgc
721 agtttgcaaa agaacatggt tacaaggtgt ttttcattga gtccatttgt aatgaccctg
781 gcataattgc agaaaacatc aggcaagtga aacttggcag ccctgattat atagactgtg
841 accgggaaaa ggttctggaa gactttctaa agagaattga gtgctatgag gtcaactacc
901 agcccttgga tgaggaactg gacagccacc tgtcctacat caagatcttc gacgtgggca
961 cacgctacat ggtgaaccga gtgcaggatc acatccagag ccgcacagtc tactacctca
1021 tgaatatcca tgtcacacct cgctccatct acctttgccg acatggcgag agtgaactca
1081 acatcagagg ccgcatcgga ggtgactctg gcctctcagt tcgcggcaag cagtatgcct
1141 atgccctggc caacttcatt cagtcccagg gcatcagctc cctgaaggtg tggaccagtc
1201 acatgaagag gaccatccag acagctgagg ccctgggtgt cccctatgag cagtggaagg
1261 ccctgaatga gattgatgcg ggtgtctgtg aggagatgac ctatgaagaa atccaggaac
1321 attaccctga agaatttgca ctgcgagacc aagataaata tcgctaccgc tatcccaagg
1381 gagagtccta tgaggatctg gttcagcgtc tggagccagt gataatggag ctagaacgac
1441 aggagaatgt actggtgatc tgccaccagg ctgtcatgcg gtgcctcctg gcctatttcc
1501 tggataaaag ttcagatgag cttccatatc tcaagtgccc tctgcacaca gtgctcaaac
1561 tcactcctgt ggcttatggc tgcaaagtgg aatccatcta cctgaatgtg gaggccgtga
1621 acacacaccg ggagaagcct gagaatgtgg acatcacccg ggaacctgag gaagccctgg
1681 atactgtccc agcccactac tga
//