LOCUS AK313719 1230 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ94315, Homo sapiens ribonucleotide reductase
M2 polypeptide (RRM2), mRNA.
ACCESSION AK313719
VERSION AK313719.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1230)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1230
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM2002910"
/clone_lib="NT2RM2"
/db_xref="H-InvDB:HIT000432585"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 61..1230
/note="Homo sapiens ribonucleotide reductase M2
polypeptide (RRM2), mRNA"
/protein_id="BAG36462.1"
/translation="MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLA
SKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFW
TAEEVDLSKDIQHWESLKPEERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARC
FYGFQIAMENIHSEMYSLLIDTYIKDPKEREFLFNAIETMPCVKKKADWALRWIGDKE
ATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLM
FKHLVHKPSEERVREIIINAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLE
LGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSSPTENSFTLDADF"
BASE COUNT 314 a 292 c 318 g 306 t
ORIGIN
1 gtgcaccctg tcccagccgt cctgtcctgg ctgctcgctc tgcttcgctg cgccgccact
61 atgctctccc tccgtgtccc gctcgcgccc atcacggacc cgcagcagct gcagctctcg
121 ccgctgaagg ggctcagctt ggtcgacaag gagaacacgc cgccggccct gagcgggacc
181 cgcgtcctgg ccagcaagac cgcgaggagg atcttccagg agcccacgga gccgaaaact
241 aaagcagctg cccccggcgt ggaggatgag ccgctgctga gagaaaaccc ccgccgcttt
301 gtcatcttcc ccatcgagta ccatgatatc tggcagatgt ataagaaggc agaggcttcc
361 ttttggaccg ccgaggaggt ggacctctcc aaggacattc agcactggga atccctgaaa
421 cccgaggaga gatattttat atcccatgtt ctggctttct ttgcagcaag cgatggcata
481 gtaaatgaaa acttggtgga gcgatttagc caagaagttc agattacaga agcccgctgt
541 ttctatggct tccaaattgc catggaaaac atacattctg aaatgtatag tcttcttatt
601 gacacttaca taaaagatcc caaagaaagg gaatttctct tcaatgccat tgaaacgatg
661 ccttgtgtca agaagaaggc agactgggcc ttgcgctgga ttggggacaa agaggctacc
721 tatggtgaac gtgttgtagc ctttgctgca gtggaaggca ttttcttttc cggttctttt
781 gcgtcgatat tctggctcaa gaaacgagga ctgatgcctg gcctcacatt ttctaatgaa
841 cttattagca gagatgaggg tttacactgt gattttgctt gcctgatgtt caaacacctg
901 gtacacaaac catcggagga gagagtaaga gaaataatta tcaatgctgt tcggatagaa
961 caggagttcc tcactgaggc cttgcctgtg aagctcattg ggatgaattg cactctaatg
1021 aagcaataca ttgagtttgt ggcagacaga cttatgctgg aactgggttt tagcaaggtt
1081 ttcagagtag agaacccatt tgactttatg gagaatattt cactggaagg aaagactaac
1141 ttctttgaga agagagtagg cgagtatcag aggatgggag tgatgtcaag tccaacagaa
1201 aattctttta ccttggatgc tgacttctaa
//