LOCUS AK313480 2308 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ94028, Homo sapiens glutamate-cysteine
ligase, catalytic subunit (GCLC),mRNA.
ACCESSION AK313480
VERSION AK313480.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2308)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2308
/clone="BRTHA3011032"
/clone_lib="BRTHA3"
/db_xref="H-InvDB:HIT000432346"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 395..2308
/note="Homo sapiens glutamate-cysteine ligase, catalytic
subunit (GCLC),mRNA"
/protein_id="BAG36264.1"
/translation="MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDV
LKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMI
EGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEV
KPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFI
ETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIV
MALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDS
YLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDA
NESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTR
VILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNST
ELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRA
SGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFR
KVKYSGSKTDSSN"
BASE COUNT 634 a 530 c 635 g 509 t
ORIGIN
1 acaagtagga agaagccagt gcaccagacc ggcaaagaga agcgggagcc gccgcggcag
61 cgcggccgtg gggtccgccg ccgccgcatc ggagcgggag gaggagcagc ggggagggcg
121 aggccgccgg gccgagagcc gtcccgcctg ctctcggtct tctgccttcg cctccgcgcg
181 gtgcgtcgga cccagggtct gtcacctggg cgccaggggc cgccgccggg gagccggagc
241 gggcaggacc ctccctccgc cgactgcggc ccgagagcgc ccccgcgggg tggagcggca
301 gccgccttct gcgggcggct gagtgtccgt ctcgcgcccg gagcgggcga ccgccgtcag
361 cccggaggag gaggaggagg aggagggggc ggccatgggg ctgctgtccc agggctcgcc
421 gctgagctgg gaggaaacca agcgccatgc cgaccacgtg cggcggcacg ggatcctcca
481 gttcctgcac atctaccacg ccgtcaagga ccggcacaag gacgttctca agtggggcga
541 tgaggtggaa tacatgttgg tatcttttga tcatgaaaat aaaaaagtcc ggttggtcct
601 gtctggggag aaagttcttg aaactctgca agagaagggg gaaaggacaa acccaaacca
661 tcctaccctt tggagaccag agtatgggag ttacatgatt gaagggacac caggacagcc
721 ctacggagga acaatgtccg agttcaatac agttgaggcc aacatgcgaa aacgccggaa
781 ggaggctact tctatattag aagaaaatca ggctctttgc acaataactt catttcccag
841 attaggctgt cctgggttca cactgcccga ggtcaaaccc aacccagtgg aaggaggagc
901 ttccaagtcc ctcttctttc cagatgaagc aataaacaag caccctcgct tcagtacctt
961 aacaagaaat atccgacata ggagaggaga aaaggttgtc atcaatgtac caatatttaa
1021 ggacaagaat acaccatctc catttataga aacatttact gaggatgatg aagcttcaag
1081 ggcttctaag ccggatcata tttacatgga tgccatggga tttggaatgg gcaattgctg
1141 tctccaggtg acattccaag cctgcagtat atctgaggcc agataccttt atgatcagtt
1201 ggctactatc tgtccaattg ttatggcttt gagtgctgca tctccctttt accgaggcta
1261 tgtgtcagac attgattgtc gctggggagt gatttctgca tctgtagatg atagaactcg
1321 ggaggagcga ggactggagc cattgaagaa caataactat aggatcagta aatcccgata
1381 tgactcaata gacagctatt tatctaagtg tggtgagaaa tataatgaca tcgacttgac
1441 gatagataaa gagatctacg aacagctgtt gcaggaaggc attgatcatc tcctggccca
1501 gcatgttgct catctcttta ttagagaccc actgacactg tttgaagaga aaatacacct
1561 ggatgatgct aatgagtctg accattttga gaatattcag tccacaaatt ggcagacaat
1621 gagatttaag ccccctcctc caaactcaga cattggatgg agagtagaat ttcgacccat
1681 ggaggtgcaa ttaacagact ttgagaactc tgcctatgtg gtgtttgtgg tactgctcac
1741 cagagtgatc ctttcctaca aattggattt tctcattcca ctgtcaaagg ttgatgagaa
1801 catgaaggta gcacagaaaa gagatgctgt cttgcaggga atgttttatt tcaggaaaga
1861 tatttgcaaa ggtggcaatg cagtggtgga tggttgtggc aaggcccaga acagcacgga
1921 gctcgctgca gaggagtaca ccctcatgag catagacacc atcatcaatg ggaaggaagg
1981 tgtgtttcct ggactgatcc caattctgaa ctcttacctt gaaaacatgg aagtggatgt
2041 ggacaccaga tgtagtattc tgaactacct aaagctaatt aagaagagag catctggaga
2101 actaatgaca gttgccagat ggatgaggga gtttatcgca aaccatcctg actacaagca
2161 agacagtgtc ataactgatg aaatgaatta tagccttatt ttgaagtgta accaaattgc
2221 aaatgaatta tgtgaatgcc cagagttact tggatcagca tttaggaaag taaaatatag
2281 tggaagtaaa actgactcat ccaactag
//