LOCUS AK313391 1910 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93925, highly similar to Homo sapiens malic
enzyme 2, NAD(+)-dependent, mitochondrial (ME2),nuclear gene
encoding mitochondrial protein, mRNA.
ACCESSION AK313391
VERSION AK313391.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1910)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1910
/clone="TRACH2020274"
/clone_lib="TRACH2"
/db_xref="H-InvDB:HIT000432257"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="trachea"
CDS 156..1910
/note="highly similar to Homo sapiens malic enzyme 2,
NAD(+)-dependent, mitochondrial (ME2),nuclear gene
encoding mitochondrial protein, mRNA"
/protein_id="BAG36189.1"
/translation="MLSRLRVVSTTCTLACRHLHIKEKGKPLMLNPRTNKGMAFTLQE
RQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQD
DIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVV
TDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFY
MGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAYRFLRKYREKYCTF
NDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSE
QEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGV
AGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFG
PVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELA
QGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLL
PDVYEWPESASSPPVITE"
BASE COUNT 589 a 370 c 458 g 493 t
ORIGIN
1 agtgtgagcc tgagctgacg gcggctccgg gaggctcgca gaaggggagg gccgggcggc
61 gcgggagctg agcatcgccg gggcgggcgg cagggcgcgg cctctccgcc gggtgtacca
121 cctgtcgcgg cgcgagacct ctggtgaaag aaaagatgtt gtcccggtta agagtagttt
181 ccaccacttg tactttggca tgtcgacatt tgcacataaa agaaaaaggc aagccactta
241 tgctgaaccc aagaacaaac aagggaatgg catttacttt acaagaacga caaatgcttg
301 gtcttcaagg acttctacct cccaaaatag agacacaaga tattcaagcc ttacgatttc
361 atagaaactt gaagaaaatg actagccctt tggaaaaata tatctacata atgggaatac
421 aagaaagaaa tgagaaattg ttttatagaa tactgcaaga tgacattgag agtttaatgc
481 caattgtata tacaccgacg gttggtcttg cctgctccca gtatggacac atctttagaa
541 gacctaaggg attatttatt tcgatctcag acagaggtca tgttagatca attgtggata
601 actggccaga aaatcatgtt aaggctgttg tagtgactga tggagagaga attctgggtc
661 ttggagatct gggtgtctat ggaatgggaa ttccagtagg aaaactttgt ttgtatacag
721 cttgtgcagg aatacggcct gatagatgcc tgccagtgtg tattgatgtg ggaactgata
781 atatcgcact cttaaaagac ccattttaca tgggcttgta ccagaaacga gatcgcacac
841 aacagtatga tgacctgatt gatgagttta tgaaagctat tactgacaga tatggccgga
901 acacactcat tcagttcgaa gactttggaa atcataatgc atacaggttc ttgagaaagt
961 accgagaaaa atattgtact ttcaatgatg atattcaagg gacagctgca gtagctctag
1021 caggtcttct tgcagcacaa aaagttatta gtaaaccaat ctccgaacac aaaatcttat
1081 tccttggagc aggagaggct gctcttggaa ttgcaaatct tatagttatg tctatggtag
1141 aaaatggcct gtcagaacaa gaggcacaaa agaaaatctg gatgtttgac aagtatggtt
1201 tattagttaa gggacggaaa gcaaaaatag atagttatca ggaaccattt actcactcag
1261 ccccagagag catacctgat acttttgaag atgcagtgaa tatactgaag ccttcaacta
1321 taattggagt tgcaggtgct ggccgtcttt tcactcctga tgtaatcaga gccatggcct
1381 ctatcaatga aaggcctgta atatttgcat taagtaatcc tacagcacag gcagagtgca
1441 cggctgaaga agcatataca cttacagagg gcaggtgttt gtttgccagt ggcagtccat
1501 ttgggccagt gaaacttaca gatgggcgag tctttacacc aggtcaagga aacaatgttt
1561 atatttttcc aggtgtggct ttagctgtta ttctctgtaa cacccggcat attagtgaca
1621 gtgttttcct agaagctgca aaggccctga caagccaatt gacagatgaa gagctagccc
1681 aagggagact ttacccaccg cttgctaata ttcaggaagt ttctattaac attgctatta
1741 aagttacaga atacctatat gctaataaaa tggctttccg atacccagaa cctgaagaca
1801 aggccaaata tgttaaagaa agaacatggc ggagtgaata tgattccctg ctgccagatg
1861 tgtatgaatg gccagaatct gcatcaagcc ctcctgtgat aacagaatag
//