LOCUS AK313206 2585 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93707, highly similar to Homo sapiens
phosphorylase, glycogen; liver (Hers disease, glycogenstorage
disease type VI) (PYGL), mRNA.
ACCESSION AK313206
VERSION AK313206.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2585)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2585
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2010695"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000432072"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 42..2585
/note="highly similar to Homo sapiens phosphorylase,
glycogen; liver (Hers disease, glycogenstorage disease
type VI) (PYGL), mRNA"
/protein_id="BAG36022.1"
/translation="MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDR
NVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMIN
LGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI
RYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWID
TQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENI
SRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQV
AIQMNDTHPALAIPELMRIFVDIGKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDL
VEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGS
HAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKI
GEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDV
QVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLI
TSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKF
MLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELK
LVIDQIDNGFFSPKQPDLFKDIISMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAW
NTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN"
BASE COUNT 728 a 619 c 660 g 578 t
ORIGIN
1 agctctctgc gcagcccgcc gcgcagcccg ccgccccagc catggcgaag cccctgacgg
61 accaggagaa gcggcggcag atcagcatcc gcggcatcgt gggcgtggag aacgtggcag
121 agctgaagaa gagtttcaac cggcacctgc acttcacgct ggtcaaggac cgcaacgtgg
181 ccaccacccg cgactactac ttcgcgctgg cgcacacggt gcgcgaccac ctggtggggc
241 gctggatccg cacgcagcag cactactacg acaagtgccc caagagggta tattacctct
301 ctctggaatt ttacatgggc cgaacattac agaacaccat gatcaacctc ggtctgcaaa
361 atgcctgtga tgaggccatt taccagcttg gattggatat agaagagtta gaagaaattg
421 aagaagatgc tggacttggc aatggtggtc ttgggagact tgctgcctgc ttcttggatt
481 ccatggcaac cctgggactt gcagcctatg gatacggcat tcggtatgaa tatgggattt
541 tcaatcagaa gatccgagat ggatggcagg tagaagaagc agatgattgg ctcagatatg
601 gaaacccttg ggagaagtcc cgcccagaat tcatgctgcc tgtgcacttc tatggaaaag
661 tagaacacac caacaccggg accaagtgga ttgacactca agtggtcctg gctctgccat
721 atgacacccc cgtgcccggc tacatgaata acactgtcaa caccatgcgc ctctggtctg
781 ctcgggcacc aaatgacttt aacctcagag actttaatgt tggagactac attcaggctg
841 tgctggaccg aaacctggcc gagaacatct cccgggtcct ctatcccaat gacaattttt
901 ttgaagggaa ggagctaaga ttgaagcagg aatactttgt ggtggctgca accttgcaag
961 atatcatccg ccgtttcaaa gcctccaagt ttggctccac ccgtggtgca ggaactgtgt
1021 ttgatgcctt cccggatcag gtggccatcc agatgaatga cactcaccct gcactcgcga
1081 tccctgagct gatgaggatt tttgtggata ttggaaaact gccctggtcc aaggcatggg
1141 agctcaccca gaagaccttc gcctacacca accacacagt gctcccggaa gccctggagc
1201 gctggcccgt ggacctggtg gagaagctgc tccctcgaca tttggaaatc atttatgaga
1261 taaatcagaa gcatttagat agaattgtgg ccttgtttcc taaagatgtg gaccgtctga
1321 gaaggatgtc tctgatagaa gaggaaggaa gcaaaaggat caacatggcc catctctgca
1381 ttgtcggttc ccatgctgtg aatggcgtgg ctaaaatcca ctcagacatc gtgaagacta
1441 aagtattcaa ggacttcagt gagctagaac ctgacaagtt tcagaataaa accaatggga
1501 tcactccaag gcgctggctc ctactctgca acccaggact tgcagagctc atagcagaga
1561 aaattggaga agactatgtg aaagacctga gccagctgac gaagctccac agcttcctgg
1621 gtgatgatgt cttcctccgg gaactcgcca aggtgaagca ggagaataag ctgaagtttt
1681 ctcagttcct ggagacggag tacaaagtga agatcaaccc atcctccatg tttgatgtcc
1741 aggtgaagag gatacatgag tacaagcgac agctcttgaa ctgtctgcat gtgatcacga
1801 tgtacaaccg cattaagaaa gaccctaaga agttattcgt gccaaggaca gttatcattg
1861 gtggtaaagc tgccccagga tatcacatgg ccaaaatgat cataaagctg atcacttcag
1921 tggcagatgt ggtgaacaat gaccctatgg ttggaagcaa gttgaaagtc atcttcttgg
1981 agaactacag agtatctctt gctgaaaaag tcattccagc cacagatctg tcagagcaga
2041 tttccactgc aggcaccgaa gcctcgggga caggcaatat gaagttcatg ctaaatgggg
2101 ccctaactat cgggaccatg gatggggcca atgtggaaat ggcagaagaa gctggggaag
2161 agaacctgtt catctttggc atgaggatag atgatgtggc tgctttggac aagaaagggt
2221 acgaggcaaa agaatactat gaggcacttc cagagctgaa gctggtcatt gatcaaattg
2281 acaatggctt tttttctccc aagcagcctg acctcttcaa agatatcatc agcatgctat
2341 tttatcatga caggtttaaa gtctttgcag actacgaagc ctatgtcaag tgtcaagata
2401 aagtgagtca gctgtacatg aatccaaagg cctggaacac aatggtactc aaaaacatag
2461 ctgcctcggg gaaattctcc agtgaccgaa caattaaaga atatgcccaa aacatctgga
2521 acgtggaacc ttcagatcta aagatttctc tatccaatga atctaacaaa gtcaatggaa
2581 attga
//