LOCUS AK313191 1464 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93690, Homo sapiens dihydrolipoamide
branched chain transacylase (E2component of branched chain keto
acid dehydrogenase complex; maplesyrup urine disease) (DBT), mRNA.
ACCESSION AK313191
VERSION AK313191.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1464)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1464
/clone="KIDNE2013618"
/clone_lib="KIDNE2"
/db_xref="H-InvDB:HIT000432057"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS 16..1464
/note="Homo sapiens dihydrolipoamide branched chain
transacylase (E2component of branched chain keto acid
dehydrogenase complex; maplesyrup urine disease) (DBT),
mRNA"
/protein_id="BAG36008.1"
/translation="MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPS
FKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQ
SDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDE
HTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILP
PSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHF
GYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQ
NITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLT
GGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSA
DHRVIDGATMSRFSNLWKSYLENPAFMLLDLK"
BASE COUNT 453 a 290 c 311 g 410 t
ORIGIN
1 attttccggg gtaagatggc tgcagtccgt atgctgagaa cctggagcag gaatgcgggg
61 aagctgattt gtgttcgcta ttttcaaaca tgtggtaatg ttcatgtttt gaagccaaat
121 tatgtgtgtt tctttggtta tccttcattc aagtatagtc atccacatca cttcctgaaa
181 acaactgctg ctctccgtgg acaggttgtt cagttcaagc tctcagacat tggagaaggg
241 attagagaag taactgttaa agaatggtat gtaaaagaag gagatacagt gtctcagttt
301 gatagcatct gtgaagttca aagtgataaa gcttctgtta ccatcactag tcgttatgat
361 ggagtcatta aaaaactcta ttataatcta gacgatattg cctatgtggg gaagccatta
421 gtagacatag aaacggaagc tttaaaagat tcagaagaag atgttgttga aactcctgca
481 gtgtctcatg atgaacatac acaccaagag ataaagggcc gaaaaacact ggcaactcct
541 gcagttcgcc gtctggcaat ggaaaacaat attaagctga gtgaagttgt tggctcagga
601 aaagatggca gaatacttaa agaagatatc ctcaactatt tggaaaagca gacaggagct
661 atattgcctc cttcacccaa agttgaaatt atgccacctc caccaaagcc aaaagacatg
721 actgttccta tactagtatc aaaacctccg gtattcacag gcaaagacaa aacagaaccc
781 ataaaaggct ttcaaaaagc aatggtcaag actatgtctg cagccctgaa gatacctcat
841 tttggttatt gtgatgagat tgaccttact gaactggtta agctccgaga agaattaaaa
901 cccattgcat ttgctcgtgg aattaaactc tcctttatgc ctttcttctt aaaggctgct
961 tccttgggat tactacagtt tcctatcctt aacgcttctg tggatgaaaa ctgccagaat
1021 ataacatata aggcttctca taacattggg atagcaatgg atactgagca gggtttgatt
1081 gtccctaatg tgaaaaatgt tcagatctgc tctatatttg acatcgccac tgaactgaac
1141 cgcctccaga aattgggctc tgtgggtcag ctcagcacca ctgatcttac aggaggaaca
1201 tttactcttt ccaacattgg atcaattggt ggtacctttg ccaaaccagt gataatgcca
1261 cctgaagtag ccattggggc ccttggatca attaaggcca ttccccgatt taaccagaaa
1321 ggagaagtat ataaggcaca gataatgaat gtgagctggt cagctgatca cagagttatt
1381 gatggtgcta caatgtcacg cttctccaat ttgtggaaat cctatttaga aaacccagct
1441 tttatgctac tagatctgaa atga
//