LOCUS AK313184 2063 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93683, Homo sapiens kinetochore associated 2
(KNTC2), mRNA.
ACCESSION AK313184
VERSION AK313184.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2063)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
The reason of remaining 5'-end "N": This sequence was generated
from 5'-end region of cDNA insert including about 20 base of the
sequence before "N". This cDNA was obtained from oligo-capped cDNA
library by which method 5'-end of mRNA was efficiently cloned.
Therefore remaining 5'-end "N" is important for a detection of
transcription start site of this mRNA.
FEATURES Location/Qualifiers
source 1..2063
/cell_line="IMR32"
/cell_type="neuroblastoma"
/clone="IMR322017956"
/clone_lib="IMR322"
/db_xref="H-InvDB:HIT000432050"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 135..2063
/note="Homo sapiens kinetochore associated 2 (KNTC2),
mRNA"
/protein_id="BAG36001.1"
/translation="MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLS
INKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEF
LTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGY
PFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDG
IMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALN
EQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEE
IARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQ
KLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCL
VKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQ
KLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQL
VVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIK
EIRDKYEKKATLIKSSEE"
BASE COUNT 728 a 358 c 469 g 507 t
ORIGIN
1 nttcgaacgg ctttggcggg ccgaggaagg acctggtgtt ttgatgaccg ctgtcctgtc
61 tagcagatac ttgcacggtt tacagaaatt cggtccctgg gtcgtgtcag gaaactggaa
121 aaaaggtcat aatcatgaag cgcagttcag tttccagcgg tggtgctggc cgcctctcca
181 tgcaggagtt aagatcccag gatgtaaata aacaaggcct ctatacccct caaaccaaag
241 agaaaccaac ctttggaaag ttgagtataa acaaaccgac atctgaaaga aaagtctcgc
301 tatttggcaa aagaactagt ggacatggat cccggaatag tcaacttggt atattttcca
361 gttctgagaa aatcaaggac ccgagaccac ttaatgacaa agcattcatt cagcagtgta
421 ttcgacaact ctgtgagttt cttacagaaa atggttatgc acataatgtg tccatgaaat
481 ctctacaagc tccctctgtt aaagacttcc tgaagatctt cacgtttctt tatggcttcc
541 tgtgcccctc atacgaactt cctgacacaa agtttgaaga agaggttcca agaatcttta
601 aagaccttgg gtatcctttt gcactatcca aaagctccat gtacacagtg ggggctcctc
661 atacatggcc tcacattgtg gcagccttag tttggctaat agactgcatc aagatacata
721 ctgccatgaa agaaagctca cctttatttg atgatgggca gccttgggga gaagaaactg
781 aagatggaat tatgcataat aagttgtttt tggactacac cataaaatgc tatgagagtt
841 ttatgagtgg tgccgacagc tttgatgaga tgaatgcaga gctgcagtca aaactgaagg
901 atttatttaa tgtggatgct tttaagctgg aatcattaga agcaaaaaac agagcattga
961 atgaacagat tgcaagattg gaacaagaaa gagaaaaaga accgaatcgt ctagagtcgt
1021 tgagaaaact gaaggcttcc ttacaaggag atgttcaaaa gtatcaggca tacatgagca
1081 atttggagtc tcattcagcc attcttgacc agaaattaaa tggtctcaat gaggaaattg
1141 ctagagtaga actagaatgt gaaacaataa aacaggagaa cactcgacta cagaatatca
1201 ttgacaacca gaagtactca gttgcagaca ttgagcgaat aaatcatgaa agaaatgaat
1261 tgcagcagac tattaataaa ttaaccaagg acctggaagc tgaacaacag aagttgtgga
1321 atgaggagtt aaaatatgcc agaggcaaag aagcgattga aacacaatta gcagagtatc
1381 acaaattggc tagaaaatta aaacttattc ctaaaggtgc tgagaattcc aaaggttatg
1441 actttgaaat taagtttaat cccgaggctg gtgccaactg ccttgtcaaa tacagggctc
1501 aagtttatgt acctcttaag gaactcctga atgaaactga agaagaaatt aataaagccc
1561 taaataaaaa aatgggtttg gaggatactt tagaacaatt gaatgcaatg ataacagaaa
1621 gcaagagaag tgtgagaact ctgaaagaag aagttcaaaa gctggatgat ctttaccaac
1681 aaaaaattaa ggaagcagag gaagaggatg aaaaatgtgc cagtgagctt gagtccttgg
1741 agaaacacaa gcacctgcta gaaagtactg ttaaccaggg gctcagtgaa gctatgaatg
1801 aattagatgc tgttcagcgg gaataccaac tagttgtgca aaccacgact gaagaaagac
1861 gaaaagtggg aaataacttg caacgtctgt tagagatggt tgctacacat gttgggtctg
1921 tagagaaaca tcttgaggag cagattgcta aagttgatag agaatatgaa gaatgcatgt
1981 cagaagatct ctcggaaaat attaaagaga ttagagataa gtatgagaag aaagctactc
2041 taattaagtc ttctgaagaa tga
//